Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix
Objective: The goal of the study is to determine the structural characteristics, mechanical properties, cytotoxicity, and biocompatibility of the pulmonary visceral pleura (PVP).Background: Collagen and elastin are the major components of the extracellular matrix. The PVP has an abundance of elastin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.796076/full |
| _version_ | 1819159605310128128 |
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| author | Xiao Lu Ling Han Ghassan S. Kassab |
| author_facet | Xiao Lu Ling Han Ghassan S. Kassab |
| author_sort | Xiao Lu |
| collection | DOAJ |
| description | Objective: The goal of the study is to determine the structural characteristics, mechanical properties, cytotoxicity, and biocompatibility of the pulmonary visceral pleura (PVP).Background: Collagen and elastin are the major components of the extracellular matrix. The PVP has an abundance of elastin and collagen that can serve as a potential biomaterial for clinical repair and reconstructions.Methods: The PVP was processed from swine and bovine lungs. Chemical analyses were used to determine collagen and elastin contents in the PVPs. Immunofluorescence microscopy was used to analyze the structure of the PVP. The stress–strain relationships and stress relaxation were determined by using the planar uniaxial test. The cytotoxicity of the PVP was tested in cultured cells. In in vivo evaluations, the PVP was implanted in the sciatic nerve and skin of rats.Results: Collagen and elastin contents are abundant in the PVP with larger proportions of elastin than in the bovine pericardium and porcine small intestinal submucosa. A microstructural analysis revealed that the elastin fibers were distributed throughout the PVP and the collagen was distributed mainly in the mesothelial basal lamina. The incremental moduli in stress–strain curves and relaxation moduli in the Maxwell–Wiechert model of PVP were approximately one-tenth of the bovine pericardium and small intestinal submucosa. The minimal cytotoxicity of the PVP was demonstrated. The axons proliferated in the PVP conduit guidance from proximal to distal sciatic nerves of rats. The neo-skin regenerated under the PVP skin substitute within 4 weeks.Conclusions: The PVP is composed of abundant collagen and elastin. The structural characteristics and mechanical compliance of the PVP render a suitable biological material for repair/reconstruction. |
| first_indexed | 2024-12-22T16:43:13Z |
| format | Article |
| id | doaj.art-b6c64e89bd1044f89242bfc3c42ba69d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-22T16:43:13Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b6c64e89bd1044f89242bfc3c42ba69d2022-12-21T18:19:49ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.796076796076Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular MatrixXiao LuLing HanGhassan S. KassabObjective: The goal of the study is to determine the structural characteristics, mechanical properties, cytotoxicity, and biocompatibility of the pulmonary visceral pleura (PVP).Background: Collagen and elastin are the major components of the extracellular matrix. The PVP has an abundance of elastin and collagen that can serve as a potential biomaterial for clinical repair and reconstructions.Methods: The PVP was processed from swine and bovine lungs. Chemical analyses were used to determine collagen and elastin contents in the PVPs. Immunofluorescence microscopy was used to analyze the structure of the PVP. The stress–strain relationships and stress relaxation were determined by using the planar uniaxial test. The cytotoxicity of the PVP was tested in cultured cells. In in vivo evaluations, the PVP was implanted in the sciatic nerve and skin of rats.Results: Collagen and elastin contents are abundant in the PVP with larger proportions of elastin than in the bovine pericardium and porcine small intestinal submucosa. A microstructural analysis revealed that the elastin fibers were distributed throughout the PVP and the collagen was distributed mainly in the mesothelial basal lamina. The incremental moduli in stress–strain curves and relaxation moduli in the Maxwell–Wiechert model of PVP were approximately one-tenth of the bovine pericardium and small intestinal submucosa. The minimal cytotoxicity of the PVP was demonstrated. The axons proliferated in the PVP conduit guidance from proximal to distal sciatic nerves of rats. The neo-skin regenerated under the PVP skin substitute within 4 weeks.Conclusions: The PVP is composed of abundant collagen and elastin. The structural characteristics and mechanical compliance of the PVP render a suitable biological material for repair/reconstruction.https://www.frontiersin.org/articles/10.3389/fbioe.2022.796076/fulllungstructurecompliancebiomechanicsbiocompatibilitynerve |
| spellingShingle | Xiao Lu Ling Han Ghassan S. Kassab Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix Frontiers in Bioengineering and Biotechnology lung structure compliance biomechanics biocompatibility nerve |
| title | Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix |
| title_full | Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix |
| title_fullStr | Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix |
| title_full_unstemmed | Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix |
| title_short | Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix |
| title_sort | pulmonary visceral pleura biomaterial elastin and collagen based extracellular matrix |
| topic | lung structure compliance biomechanics biocompatibility nerve |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.796076/full |
| work_keys_str_mv | AT xiaolu pulmonaryvisceralpleurabiomaterialelastinandcollagenbasedextracellularmatrix AT linghan pulmonaryvisceralpleurabiomaterialelastinandcollagenbasedextracellularmatrix AT ghassanskassab pulmonaryvisceralpleurabiomaterialelastinandcollagenbasedextracellularmatrix |