Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces
In orthopedic, dental, and maxillofacial fields, joint prostheses, plates, and screws are widely used in the treatment of problems related to bone tissue. However, the use of these prosthetic systems is not free from complications: the fibrotic encapsulation of endosseous implants often prevents opt...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8727 |
| _version_ | 1827637626795982848 |
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| author | Annj Zamuner Antonella Pasquato Ignazio Castagliuolo Monica Dettin Paola Brun |
| author_facet | Annj Zamuner Antonella Pasquato Ignazio Castagliuolo Monica Dettin Paola Brun |
| author_sort | Annj Zamuner |
| collection | DOAJ |
| description | In orthopedic, dental, and maxillofacial fields, joint prostheses, plates, and screws are widely used in the treatment of problems related to bone tissue. However, the use of these prosthetic systems is not free from complications: the fibrotic encapsulation of endosseous implants often prevents optimal integration of the prostheses with the surrounding bone. To overcome these issues, biomimetic titanium implants have been developed where synthetic peptides have been selectively grafted on titanium surfaces via Schiff base formation. We used the retro-inverted sequence (DHVPX) from [351–359] human Vitronectin and its dimer (D2HVP). Both protease-resistant peptides showed increased human osteoblast adhesion and proliferation, an augmented number of focal adhesions, and cellular spreading with respect to the control. D2HVP-grafted samples significantly enhance Secreted Phosphoprotein 1, Integrin Binding Sialoprotein, and Vitronectin gene expression vs. control. An estimation of peptide surface density was determined by Two-photon microscopy analysis on a silanized glass model surface labeled with a fluorescent analog. |
| first_indexed | 2024-03-09T16:03:33Z |
| format | Article |
| id | doaj.art-b6c6fdbb5928478eadcdfcb405af3fbd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:03:33Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b6c6fdbb5928478eadcdfcb405af3fbd2023-11-24T16:56:29ZengMDPI AGMolecules1420-30492022-12-012724872710.3390/molecules27248727Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium SurfacesAnnj Zamuner0Antonella Pasquato1Ignazio Castagliuolo2Monica Dettin3Paola Brun4Department of Civil, Environmental and Architectural Engineering, University of Padua, 35131 Padua, ItalyDepartment of Industrial Engineering, University of Padua, 35131 Padua, ItalyDepartment of Molecular Medicine, University of Padua, 35121 Padua, ItalyDepartment of Industrial Engineering, University of Padua, 35131 Padua, ItalyDepartment of Molecular Medicine, University of Padua, 35121 Padua, ItalyIn orthopedic, dental, and maxillofacial fields, joint prostheses, plates, and screws are widely used in the treatment of problems related to bone tissue. However, the use of these prosthetic systems is not free from complications: the fibrotic encapsulation of endosseous implants often prevents optimal integration of the prostheses with the surrounding bone. To overcome these issues, biomimetic titanium implants have been developed where synthetic peptides have been selectively grafted on titanium surfaces via Schiff base formation. We used the retro-inverted sequence (DHVPX) from [351–359] human Vitronectin and its dimer (D2HVP). Both protease-resistant peptides showed increased human osteoblast adhesion and proliferation, an augmented number of focal adhesions, and cellular spreading with respect to the control. D2HVP-grafted samples significantly enhance Secreted Phosphoprotein 1, Integrin Binding Sialoprotein, and Vitronectin gene expression vs. control. An estimation of peptide surface density was determined by Two-photon microscopy analysis on a silanized glass model surface labeled with a fluorescent analog.https://www.mdpi.com/1420-3049/27/24/8727titaniumselective graftingosteoblastsprotease-resistant peptidesretro-inverted peptidesadhesive peptides |
| spellingShingle | Annj Zamuner Antonella Pasquato Ignazio Castagliuolo Monica Dettin Paola Brun Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces Molecules titanium selective grafting osteoblasts protease-resistant peptides retro-inverted peptides adhesive peptides |
| title | Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces |
| title_full | Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces |
| title_fullStr | Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces |
| title_full_unstemmed | Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces |
| title_short | Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces |
| title_sort | selective grafting of protease resistant adhesive peptides on titanium surfaces |
| topic | titanium selective grafting osteoblasts protease-resistant peptides retro-inverted peptides adhesive peptides |
| url | https://www.mdpi.com/1420-3049/27/24/8727 |
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