Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain model
IntroductionAllodynia, which can be induced by paclitaxel administration, is the presence of pain as a result of a stimulus that does not usually provoke pain. Many studies have investigated the analgesic efficacy of acupuncture, including laser acupuncture (LA) and electroacupuncture (EA). Although...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1153903/full |
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| author | Chan-Suk Yoon Ga-Won Lee Myeong-Hun Kim Sang-Mi Kang Cha-Kyung Youn Ji-Hye Yang Eun-Ju Kim Hong-Seok Son Sok Cheon Pak Seon-Jong Kim Chang-Su Na |
| author_facet | Chan-Suk Yoon Ga-Won Lee Myeong-Hun Kim Sang-Mi Kang Cha-Kyung Youn Ji-Hye Yang Eun-Ju Kim Hong-Seok Son Sok Cheon Pak Seon-Jong Kim Chang-Su Na |
| author_sort | Chan-Suk Yoon |
| collection | DOAJ |
| description | IntroductionAllodynia, which can be induced by paclitaxel administration, is the presence of pain as a result of a stimulus that does not usually provoke pain. Many studies have investigated the analgesic efficacy of acupuncture, including laser acupuncture (LA) and electroacupuncture (EA). Although pain-related diseases are relatively common, few studies have analyzed the analgesic effects and mechanisms of LA combined with EA. The purpose of this study was to investigate the therapeutic effect and mechanism of manual acupuncture (MA), EA, LA, and combined therapy (LA + EA) in a paclitaxel-induced allodynia rat model.MethodsA total of 56 rats were classified into eight groups: a normal (Nor, n = 7), a control (Con, n = 7), an MA (n = 7), an EA (n = 7), a 650-nm LA (650LA, n = 7), an 830-nm LA (830LA, n = 7), a 650-nm LA combined with EA (650LA + EA, n = 7), and an 830-nm LA combined with EA group (830LA + EA, n = 7). Allodynia was induced by intraperitoneal injection of 2 mg/kg of paclitaxel every other day for a total of four times except the Nor group. Acupuncture treatments were conducted at the points of Jungwan (CV12) and Joksamni (ST36) once every other day for 6 min, for a total of nine times. Withdrawal response reaction times and force intensity of the foot were measured before the start of the experiment, after the 4th paclitaxel administration (day 8), and after the 9th and last treatment (day 15). On the 16th day, mRNA and protein expression in the spinal nerves was assessed, and a metabolome analysis of the animals’ feces was performed.Results and discussionOur analyses show that 650LA + EA treatment resulted in an upregulation of protein expression related to pain relief and nerve regeneration, whereas 830LA + EA treatment led to significant changes in metabolomes. This study demonstrates that a combination treatment of EA and LA can suppress allodynia and promote upregulation of protein expression related to nerve regeneration and is effective in changing the intestinal microbiome. Further large-scale research is required to assess the exact mechanism underlying the therapeutic effect of this combination treatment in pain-related diseases. |
| first_indexed | 2024-04-09T17:31:49Z |
| format | Article |
| id | doaj.art-b6c83ac3db6e4e6f8ae28369f8d3188a |
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| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-09T17:31:49Z |
| publishDate | 2023-04-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b6c83ac3db6e4e6f8ae28369f8d3188a2023-04-18T04:54:52ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-04-011010.3389/fvets.2023.11539031153903Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain modelChan-Suk Yoon0Ga-Won Lee1Myeong-Hun Kim2Sang-Mi Kang3Cha-Kyung Youn4Ji-Hye Yang5Eun-Ju Kim6Hong-Seok Son7Sok Cheon Pak8Seon-Jong Kim9Chang-Su Na10School of Korean Medicine, Dongshin University, Naju, Jeonnam, Republic of KoreaDepartment of Companion Animal Industry, College of Health and Welfare, Dongshin University, Naju, Jeonnam, Republic of KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam, Republic of KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam, Republic of KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam, Republic of KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam, Republic of KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of KoreaSchool of Biomedical Sciences, Charles Sturt University, Bathurst, NSW, AustraliaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam, Republic of KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam, Republic of KoreaIntroductionAllodynia, which can be induced by paclitaxel administration, is the presence of pain as a result of a stimulus that does not usually provoke pain. Many studies have investigated the analgesic efficacy of acupuncture, including laser acupuncture (LA) and electroacupuncture (EA). Although pain-related diseases are relatively common, few studies have analyzed the analgesic effects and mechanisms of LA combined with EA. The purpose of this study was to investigate the therapeutic effect and mechanism of manual acupuncture (MA), EA, LA, and combined therapy (LA + EA) in a paclitaxel-induced allodynia rat model.MethodsA total of 56 rats were classified into eight groups: a normal (Nor, n = 7), a control (Con, n = 7), an MA (n = 7), an EA (n = 7), a 650-nm LA (650LA, n = 7), an 830-nm LA (830LA, n = 7), a 650-nm LA combined with EA (650LA + EA, n = 7), and an 830-nm LA combined with EA group (830LA + EA, n = 7). Allodynia was induced by intraperitoneal injection of 2 mg/kg of paclitaxel every other day for a total of four times except the Nor group. Acupuncture treatments were conducted at the points of Jungwan (CV12) and Joksamni (ST36) once every other day for 6 min, for a total of nine times. Withdrawal response reaction times and force intensity of the foot were measured before the start of the experiment, after the 4th paclitaxel administration (day 8), and after the 9th and last treatment (day 15). On the 16th day, mRNA and protein expression in the spinal nerves was assessed, and a metabolome analysis of the animals’ feces was performed.Results and discussionOur analyses show that 650LA + EA treatment resulted in an upregulation of protein expression related to pain relief and nerve regeneration, whereas 830LA + EA treatment led to significant changes in metabolomes. This study demonstrates that a combination treatment of EA and LA can suppress allodynia and promote upregulation of protein expression related to nerve regeneration and is effective in changing the intestinal microbiome. Further large-scale research is required to assess the exact mechanism underlying the therapeutic effect of this combination treatment in pain-related diseases.https://www.frontiersin.org/articles/10.3389/fvets.2023.1153903/fulllaser-and electro-acupuncture combined therapypaclitaxelallodyniaendocannabinoidsmetabolome analyses |
| spellingShingle | Chan-Suk Yoon Ga-Won Lee Myeong-Hun Kim Sang-Mi Kang Cha-Kyung Youn Ji-Hye Yang Eun-Ju Kim Hong-Seok Son Sok Cheon Pak Seon-Jong Kim Chang-Su Na Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain model Frontiers in Veterinary Science laser-and electro-acupuncture combined therapy paclitaxel allodynia endocannabinoids metabolome analyses |
| title | Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain model |
| title_full | Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain model |
| title_fullStr | Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain model |
| title_full_unstemmed | Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain model |
| title_short | Analgesic effects and metabolome analyses of laser- and electro-acupuncture combined therapies in paclitaxel-induced neuropathic pain model |
| title_sort | analgesic effects and metabolome analyses of laser and electro acupuncture combined therapies in paclitaxel induced neuropathic pain model |
| topic | laser-and electro-acupuncture combined therapy paclitaxel allodynia endocannabinoids metabolome analyses |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1153903/full |
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