Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5
Transgenic plums, Prunus domestica L. clone C5, were inoculated by bud grafting with Plum pox virus (PPV-Rec, recombinant strain originated from plum), PPV-Rec + Apple chlorotic leafspot virus (ACLSV), PPV-Rec + Prune dwarf virus (PDV), and PPV-Rec + ACLSV + PDV. Non-inoculated transgenic plums serv...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2008-03-01
|
| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200801-0001_interactions-of-plum-pox-virus-strain-rec-with-apple-chlorotic-leafspot-virus-and-prune-dwarf-viruses-in-field.php |
| _version_ | 1797898583462117376 |
|---|---|
| author | Jaroslav POLÁK Michel RAVELONANDRO Jiban KUMAR-KUNDU Jitka PÍVALOVÁ Ralph SCORZA |
| author_facet | Jaroslav POLÁK Michel RAVELONANDRO Jiban KUMAR-KUNDU Jitka PÍVALOVÁ Ralph SCORZA |
| author_sort | Jaroslav POLÁK |
| collection | DOAJ |
| description | Transgenic plums, Prunus domestica L. clone C5, were inoculated by bud grafting with Plum pox virus (PPV-Rec, recombinant strain originated from plum), PPV-Rec + Apple chlorotic leafspot virus (ACLSV), PPV-Rec + Prune dwarf virus (PDV), and PPV-Rec + ACLSV + PDV. Non-inoculated transgenic plums served as controls. Plants were grown in an open field for 5 years. They were evaluated by visible symptoms, by DAS-ELISA and RT-PCR. Mild PPV symptoms, diffuse spots or rings appeared two years after inoculation in some leaves of plants artificially inoculated with PPV-Rec, PPV-Rec + ACLSV, PPV-Rec + PDV, and PPV-Rec + ACLSV + PDV. Severe PPV symptoms appeared in leaves of shoots growing from infected buds used for inoculation. During the following three years, further weakening of PPV symptoms was observed in transgenic plants. In 2007, very mild PPV symptoms were found in only a few leaves, and over 60%, resp. 70% of the C5 trees showed no PPV symptoms. The presence of PPV was confirmed by ELISA, ISEM and RT-PCR. No difference in PPV symptoms was observed between PPV-Rec and combinations PPV-Rec + ACLSV, PPV-Rec + PDV, PPV-Rec + ACLSV + PDV. No symptoms of ACLSV appeared in combinations of ACLSV with PPV-Rec and PPV-Rec + PDV during 2004-2007, but the presence of ACLSV in leaves of transgenic plants clone C5 was proved by ELISA and RT-PCR. Neither synergistic nor antagonistic effects of ACLSV on PPV-Rec were observed. No symptoms of PDV appeared in combinations of viruses with PDV during 2004-2007. PDV was not detected by ELISA, and the presence of PDV was uncertain by RT-PCR in most of inoculated trees in 2006 and 2007. The results of RT-PCR will be further confirmed by sequence analysis and discussed. These results suggest a possible antagonistic interaction between PPV-Rec and PDV in plum clone C5. |
| first_indexed | 2024-04-10T08:16:34Z |
| format | Article |
| id | doaj.art-b6ca82cbf5604f5cb2b70840832e24fe |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:16:34Z |
| publishDate | 2008-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-b6ca82cbf5604f5cb2b70840832e24fe2023-02-23T03:43:52ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412008-03-014411510.17221/535-PPSpps-200801-0001Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5Jaroslav POLÁK0Michel RAVELONANDRO1Jiban KUMAR-KUNDU2Jitka PÍVALOVÁ3Ralph SCORZA4Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech RepublicStation of Plant Pathology, INRA Bordeaux, FranceDepartment of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech RepublicDepartment of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech RepublicUSDA-ARS, Appalachian Fruit Research Station, Kearneysville, West Virginia, USATransgenic plums, Prunus domestica L. clone C5, were inoculated by bud grafting with Plum pox virus (PPV-Rec, recombinant strain originated from plum), PPV-Rec + Apple chlorotic leafspot virus (ACLSV), PPV-Rec + Prune dwarf virus (PDV), and PPV-Rec + ACLSV + PDV. Non-inoculated transgenic plums served as controls. Plants were grown in an open field for 5 years. They were evaluated by visible symptoms, by DAS-ELISA and RT-PCR. Mild PPV symptoms, diffuse spots or rings appeared two years after inoculation in some leaves of plants artificially inoculated with PPV-Rec, PPV-Rec + ACLSV, PPV-Rec + PDV, and PPV-Rec + ACLSV + PDV. Severe PPV symptoms appeared in leaves of shoots growing from infected buds used for inoculation. During the following three years, further weakening of PPV symptoms was observed in transgenic plants. In 2007, very mild PPV symptoms were found in only a few leaves, and over 60%, resp. 70% of the C5 trees showed no PPV symptoms. The presence of PPV was confirmed by ELISA, ISEM and RT-PCR. No difference in PPV symptoms was observed between PPV-Rec and combinations PPV-Rec + ACLSV, PPV-Rec + PDV, PPV-Rec + ACLSV + PDV. No symptoms of ACLSV appeared in combinations of ACLSV with PPV-Rec and PPV-Rec + PDV during 2004-2007, but the presence of ACLSV in leaves of transgenic plants clone C5 was proved by ELISA and RT-PCR. Neither synergistic nor antagonistic effects of ACLSV on PPV-Rec were observed. No symptoms of PDV appeared in combinations of viruses with PDV during 2004-2007. PDV was not detected by ELISA, and the presence of PDV was uncertain by RT-PCR in most of inoculated trees in 2006 and 2007. The results of RT-PCR will be further confirmed by sequence analysis and discussed. These results suggest a possible antagonistic interaction between PPV-Rec and PDV in plum clone C5.https://pps.agriculturejournals.cz/artkey/pps-200801-0001_interactions-of-plum-pox-virus-strain-rec-with-apple-chlorotic-leafspot-virus-and-prune-dwarf-viruses-in-field.phptransgenic plumresistancesharkainteractionsppvpdvaclsv |
| spellingShingle | Jaroslav POLÁK Michel RAVELONANDRO Jiban KUMAR-KUNDU Jitka PÍVALOVÁ Ralph SCORZA Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5 Plant Protection Science transgenic plum resistance sharka interactions ppv pdv aclsv |
| title | Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5 |
| title_full | Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5 |
| title_fullStr | Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5 |
| title_full_unstemmed | Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5 |
| title_short | Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5 |
| title_sort | interactions of plum pox virus strain rec with apple chlorotic leafspot virus and prune dwarf viruses in field grown transgenic plum prunus domestica l clone c5 |
| topic | transgenic plum resistance sharka interactions ppv pdv aclsv |
| url | https://pps.agriculturejournals.cz/artkey/pps-200801-0001_interactions-of-plum-pox-virus-strain-rec-with-apple-chlorotic-leafspot-virus-and-prune-dwarf-viruses-in-field.php |
| work_keys_str_mv | AT jaroslavpolak interactionsofplumpoxvirusstrainrecwithapplechloroticleafspotvirusandprunedwarfvirusesinfieldgrowntransgenicplumprunusdomesticalclonec5 AT michelravelonandro interactionsofplumpoxvirusstrainrecwithapplechloroticleafspotvirusandprunedwarfvirusesinfieldgrowntransgenicplumprunusdomesticalclonec5 AT jibankumarkundu interactionsofplumpoxvirusstrainrecwithapplechloroticleafspotvirusandprunedwarfvirusesinfieldgrowntransgenicplumprunusdomesticalclonec5 AT jitkapivalova interactionsofplumpoxvirusstrainrecwithapplechloroticleafspotvirusandprunedwarfvirusesinfieldgrowntransgenicplumprunusdomesticalclonec5 AT ralphscorza interactionsofplumpoxvirusstrainrecwithapplechloroticleafspotvirusandprunedwarfvirusesinfieldgrowntransgenicplumprunusdomesticalclonec5 |