Purification, characterization and structural determination of chitinases produced by Moniliophthora perniciosa

Purification, characterization and structural determination of chitinases produced by Moniliophthora perniciosa

The enzyme chitinase from Moniliophthora perniciosa the causative agent of the witches' broom disease in Theobroma cacao, was partially purified with ammonium sulfate and filtration by Sephacryl S-200 using sodium phosphate as an extraction buffer. Response surface methodology (RSM) was used to...

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Bibliographic Details
Main Authors: Rafaela S. Galante, Alex G. Taranto, Maria G.B. Koblitz, Aristóteles Góes-Neto, Carlos P. Pirovani, Júlio C.M. Cascardo, Sandra H. Cruz, Gonçalo A.G. Pereira, Sandra A. de Assis
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2012-06-01
Series:Anais da Academia Brasileira de Ciências
Subjects:
quitinase
Moniliophthora perniciosa
caracterização cinética
purificação
isoenzimas
estabilidade térmica
estrutura 3D
modelagem comparativa
Chitinase
kinetic characterization
purification
isoenzymes
heat stability
3D structure
comparative modeling
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200016

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200016

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