Ndel1 promotes axon regeneration via intermediate filaments.
Failure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2291557?pdf=render |
| _version_ | 1828174956351979520 |
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| author | Cory Toth Su Yeon Shim Jian Wang Yulan Jiang Gernot Neumayer Camille Belzil Wei-Qiao Liu Jose Martinez Douglas Zochodne Minh Dang Nguyen |
| author_facet | Cory Toth Su Yeon Shim Jian Wang Yulan Jiang Gernot Neumayer Camille Belzil Wei-Qiao Liu Jose Martinez Douglas Zochodne Minh Dang Nguyen |
| author_sort | Cory Toth |
| collection | DOAJ |
| description | Failure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian homologue of aspergillus nidulans NudE, Ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L. Consistent with a role for Ndel1 in the conditioning lesion-induced neurite outgrowth of Dorsal Root Ganglion (DRG) neurons, the long lasting in vivo formation of the neuronal Ndel1/Vimentin complex was associated with robust axon regeneration. Furthermore, local silencing of Ndel1 in transected axons by siRNA severely reduced the extent of regeneration in vivo. Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration. |
| first_indexed | 2024-04-12T04:20:23Z |
| format | Article |
| id | doaj.art-b6cedbbd89604537830a3c45a9224256 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:20:23Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b6cedbbd89604537830a3c45a92242562022-12-22T03:48:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0134e201410.1371/journal.pone.0002014Ndel1 promotes axon regeneration via intermediate filaments.Cory TothSu Yeon ShimJian WangYulan JiangGernot NeumayerCamille BelzilWei-Qiao LiuJose MartinezDouglas ZochodneMinh Dang NguyenFailure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian homologue of aspergillus nidulans NudE, Ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L. Consistent with a role for Ndel1 in the conditioning lesion-induced neurite outgrowth of Dorsal Root Ganglion (DRG) neurons, the long lasting in vivo formation of the neuronal Ndel1/Vimentin complex was associated with robust axon regeneration. Furthermore, local silencing of Ndel1 in transected axons by siRNA severely reduced the extent of regeneration in vivo. Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration.http://europepmc.org/articles/PMC2291557?pdf=render |
| spellingShingle | Cory Toth Su Yeon Shim Jian Wang Yulan Jiang Gernot Neumayer Camille Belzil Wei-Qiao Liu Jose Martinez Douglas Zochodne Minh Dang Nguyen Ndel1 promotes axon regeneration via intermediate filaments. PLoS ONE |
| title | Ndel1 promotes axon regeneration via intermediate filaments. |
| title_full | Ndel1 promotes axon regeneration via intermediate filaments. |
| title_fullStr | Ndel1 promotes axon regeneration via intermediate filaments. |
| title_full_unstemmed | Ndel1 promotes axon regeneration via intermediate filaments. |
| title_short | Ndel1 promotes axon regeneration via intermediate filaments. |
| title_sort | ndel1 promotes axon regeneration via intermediate filaments |
| url | http://europepmc.org/articles/PMC2291557?pdf=render |
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