Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir Grains
In this bioremediation study, the fungus <i>Penicillium</i> sp. isolated from kefir grains was evaluated for its resistance to copper in the culture medium. <i>Penicillium</i> sp. was cultivated in liquid medium prepared using 2% malt-agar at pH 7.0. Biomass of the fungus was...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1439 |
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| author | Antonio Ferreira de Oliveira Raquellyne Baia Machado Adriana Maciel Ferreira Iracirema da Silva Sena Maria Eduarda Silveira Ana Maria Santos de Almeida Francinaldo S. Braga Alex Bruno Lobato Rodrigues Roberto Messias Bezerra Irlon Maciel Ferreira Alexandro Cezar Florentino |
| author_facet | Antonio Ferreira de Oliveira Raquellyne Baia Machado Adriana Maciel Ferreira Iracirema da Silva Sena Maria Eduarda Silveira Ana Maria Santos de Almeida Francinaldo S. Braga Alex Bruno Lobato Rodrigues Roberto Messias Bezerra Irlon Maciel Ferreira Alexandro Cezar Florentino |
| author_sort | Antonio Ferreira de Oliveira |
| collection | DOAJ |
| description | In this bioremediation study, the fungus <i>Penicillium</i> sp. isolated from kefir grains was evaluated for its resistance to copper in the culture medium. <i>Penicillium</i> sp. was cultivated in liquid medium prepared using 2% malt-agar at pH 7.0. Biomass of the fungus was significantly reduced, but only when 800 mg·L<sup>−1</sup> of Cu(NO<sub>3</sub>)<sub>2</sub> copper nitrate was used. The effect on radial growth of the fungus in experiments combining different pH values and the inorganic contaminant showed an inhibition of 73% at pH 4.0, 75% at pH 7.0 and 77% at pH 9.0 in liquid medium. Thus, even though the growth of <i>Penicillium</i> sp. could be inhibited with relatively high doses of copper nitrate, images obtained with scanning electron microscopy showed the preservation of fungal cell integrity. Therefore, it can be concluded that <i>Penicillium</i> sp. isolated from kefir grains can survive while performing bioremediation to minimize the negative effects of copper on the environment through biosorption. |
| first_indexed | 2024-03-11T02:08:34Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:34Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b6d26575b8ce49e99bdaceaeace075b62023-11-18T11:41:56ZengMDPI AGMicroorganisms2076-26072023-05-01116143910.3390/microorganisms11061439Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir GrainsAntonio Ferreira de Oliveira0Raquellyne Baia Machado1Adriana Maciel Ferreira2Iracirema da Silva Sena3Maria Eduarda Silveira4Ana Maria Santos de Almeida5Francinaldo S. Braga6Alex Bruno Lobato Rodrigues7Roberto Messias Bezerra8Irlon Maciel Ferreira9Alexandro Cezar Florentino10Ichthyo and Genotoxicity Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilIchthyo and Genotoxicity Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilResearch Laboratory of Drugs, Department of Biological and Health Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilLaboratory of Biocatalysis and Applied Organic Synthesis, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilIchthyo and Genotoxicity Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilIchthyo and Genotoxicity Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilIchthyo and Genotoxicity Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilAnalytical Chemistry Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilIchthyo and Genotoxicity Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilLaboratory of Biocatalysis and Applied Organic Synthesis, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilIchthyo and Genotoxicity Laboratory, Department of Exact and Technological Sciences, Federal University of Amapá, Rod. JK, km 02, Macapá 68903-419, BrazilIn this bioremediation study, the fungus <i>Penicillium</i> sp. isolated from kefir grains was evaluated for its resistance to copper in the culture medium. <i>Penicillium</i> sp. was cultivated in liquid medium prepared using 2% malt-agar at pH 7.0. Biomass of the fungus was significantly reduced, but only when 800 mg·L<sup>−1</sup> of Cu(NO<sub>3</sub>)<sub>2</sub> copper nitrate was used. The effect on radial growth of the fungus in experiments combining different pH values and the inorganic contaminant showed an inhibition of 73% at pH 4.0, 75% at pH 7.0 and 77% at pH 9.0 in liquid medium. Thus, even though the growth of <i>Penicillium</i> sp. could be inhibited with relatively high doses of copper nitrate, images obtained with scanning electron microscopy showed the preservation of fungal cell integrity. Therefore, it can be concluded that <i>Penicillium</i> sp. isolated from kefir grains can survive while performing bioremediation to minimize the negative effects of copper on the environment through biosorption.https://www.mdpi.com/2076-2607/11/6/1439bioremediationcopper nitrate<i>Penicillium</i> sp.kefirinorganic contaminant |
| spellingShingle | Antonio Ferreira de Oliveira Raquellyne Baia Machado Adriana Maciel Ferreira Iracirema da Silva Sena Maria Eduarda Silveira Ana Maria Santos de Almeida Francinaldo S. Braga Alex Bruno Lobato Rodrigues Roberto Messias Bezerra Irlon Maciel Ferreira Alexandro Cezar Florentino Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir Grains Microorganisms bioremediation copper nitrate <i>Penicillium</i> sp. kefir inorganic contaminant |
| title | Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir Grains |
| title_full | Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir Grains |
| title_fullStr | Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir Grains |
| title_full_unstemmed | Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir Grains |
| title_short | Copper-Contaminated Substrate Biosorption by <i>Penicillium</i> sp. Isolated from Kefir Grains |
| title_sort | copper contaminated substrate biosorption by i penicillium i sp isolated from kefir grains |
| topic | bioremediation copper nitrate <i>Penicillium</i> sp. kefir inorganic contaminant |
| url | https://www.mdpi.com/2076-2607/11/6/1439 |
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