Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality
The composition of seminal plasma of individual sires varies and so does the fertilizing ability. Micro and macro elements along with seminal enzymes, hormones, proteins, and lipids contained in seminal plasma are essential for the proper physiological function of spermatozoa. However, elevated leve...
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2021-11-01
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| author | Filip Tirpák Marko Halo Katarína Tokárová Lukasz J. Binkowski Jaromír Vašíček Andrea Svoradová Martyna Błaszczyk-Altman Anton Kováčik Eva Tvrdá Peter Chrenek Norbert Lukáč Peter Massányi |
| author_facet | Filip Tirpák Marko Halo Katarína Tokárová Lukasz J. Binkowski Jaromír Vašíček Andrea Svoradová Martyna Błaszczyk-Altman Anton Kováčik Eva Tvrdá Peter Chrenek Norbert Lukáč Peter Massányi |
| author_sort | Filip Tirpák |
| collection | DOAJ |
| description | The composition of seminal plasma of individual sires varies and so does the fertilizing ability. Micro and macro elements along with seminal enzymes, hormones, proteins, and lipids contained in seminal plasma are essential for the proper physiological function of spermatozoa. However, elevated levels against the normal physiological values, especially in the case of trace metals, result in the production of reactive oxygen species. The deficiency of antioxidants in the seminal plasma that could scavenge free radicals causes an impairment of spermatozoa quality. Ejaculates were obtained from 19 stallions. The fresh semen was analyzed to evaluate qualitative parameters of spermatozoa in terms of the motility, viability, and integrity of DNA. Separated seminal plasma underwent the assessment of the chemical and biochemical composition and RedOx markers. Based on the obtained concentrations of individual chemical elements, the correlation analysis suggested a negative impact of Cu in seminal plasma on the SOD, GPx, and LPO. Contrary, positive correlation was detected between FRAP and motility features. While Cu negatively correlated with sperm motion parameters, the adverse effect on viability was suggested for Cd. Our data suggest that seminal plasma has a potential due to its availability to become the potential biomarker of the reproductive health of farm animals. |
| first_indexed | 2024-03-10T05:21:00Z |
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| id | doaj.art-b6d3fa88ae5449a38b78dc2b1879ba37 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T05:21:00Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-b6d3fa88ae5449a38b78dc2b1879ba372023-11-23T00:04:55ZengMDPI AGLife2075-17292021-11-011111123810.3390/life11111238Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa QualityFilip Tirpák0Marko Halo1Katarína Tokárová2Lukasz J. Binkowski3Jaromír Vašíček4Andrea Svoradová5Martyna Błaszczyk-Altman6Anton Kováčik7Eva Tvrdá8Peter Chrenek9Norbert Lukáč10Peter Massányi11AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaAgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Biology, Pedagogical University of Krakow, Podchorazych 2, 30-084 Krakow, PolandInstitute of Biotechnology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaNPPC—Research Institute for Animal Production Nitra, Institute of Farm Animal Genetics and Reproduction, Hlohovecka 2, 951 41 Luzianky, SlovakiaInstitute of Biology, Pedagogical University of Krakow, Podchorazych 2, 30-084 Krakow, PolandInstitute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Biotechnology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaThe composition of seminal plasma of individual sires varies and so does the fertilizing ability. Micro and macro elements along with seminal enzymes, hormones, proteins, and lipids contained in seminal plasma are essential for the proper physiological function of spermatozoa. However, elevated levels against the normal physiological values, especially in the case of trace metals, result in the production of reactive oxygen species. The deficiency of antioxidants in the seminal plasma that could scavenge free radicals causes an impairment of spermatozoa quality. Ejaculates were obtained from 19 stallions. The fresh semen was analyzed to evaluate qualitative parameters of spermatozoa in terms of the motility, viability, and integrity of DNA. Separated seminal plasma underwent the assessment of the chemical and biochemical composition and RedOx markers. Based on the obtained concentrations of individual chemical elements, the correlation analysis suggested a negative impact of Cu in seminal plasma on the SOD, GPx, and LPO. Contrary, positive correlation was detected between FRAP and motility features. While Cu negatively correlated with sperm motion parameters, the adverse effect on viability was suggested for Cd. Our data suggest that seminal plasma has a potential due to its availability to become the potential biomarker of the reproductive health of farm animals.https://www.mdpi.com/2075-1729/11/11/1238horseprooxidant activityantioxidant activitymicro and macroelementsspermatozoaDNA |
| spellingShingle | Filip Tirpák Marko Halo Katarína Tokárová Lukasz J. Binkowski Jaromír Vašíček Andrea Svoradová Martyna Błaszczyk-Altman Anton Kováčik Eva Tvrdá Peter Chrenek Norbert Lukáč Peter Massányi Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality Life horse prooxidant activity antioxidant activity micro and macroelements spermatozoa DNA |
| title | Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality |
| title_full | Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality |
| title_fullStr | Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality |
| title_full_unstemmed | Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality |
| title_short | Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality |
| title_sort | composition of stallion seminal plasma and its impact on oxidative stress markers and spermatozoa quality |
| topic | horse prooxidant activity antioxidant activity micro and macroelements spermatozoa DNA |
| url | https://www.mdpi.com/2075-1729/11/11/1238 |
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