β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, Brazil
There is a high incidence of infections caused by betalactamase-producing Gram-negative microorganisms in Brazil. These organisms are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. The present study was conducted in sentinel rectal swabs f...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-01-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867011701362 |
| _version_ | 1818305169997168640 |
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| author | Marcia Regina G. Vasques Alexandre Ribeiro Bello Cristiane da Cruz Lamas Juarez Correa José Augusto Adler Pereira |
| author_facet | Marcia Regina G. Vasques Alexandre Ribeiro Bello Cristiane da Cruz Lamas Juarez Correa José Augusto Adler Pereira |
| author_sort | Marcia Regina G. Vasques |
| collection | DOAJ |
| description | There is a high incidence of infections caused by betalactamase-producing Gram-negative microorganisms in Brazil. These organisms are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. The present study was conducted in sentinel rectal swabs from patients admitted to a cardiac surgery hospital in Rio de Janeiro, from January through December 2007, in a consecutive manner. The aim of the study was to characterize the genotype and phenotype of these isolates from colonized patients. Biochemical tests, antimicrobial susceptibility tests, a confirmatory test for the expression of extended spectrum betalactamase (ESBL) production and polymerase chain reaction for the blaTEM, blaSHV, CTX-M1, Toho-1 and AmpC genes were performed at the University Hospital of Universidade do Estado do Rio de Janeiro (UERJ). The most frequently isolated bacteria were Escherichia coli 9/41 (21.95%) and Klebsiella pneumoniae 14/41 (34.1%). In 24/41 (58%), the ESBL genotype was confirmed. The most prevalent genes in samples that expressed ESBL were blaTEM 13/24 (54%), AmpC 12/24 (50%), blaSHV 6/24 (25%), CTX-M1 7/24 (29%), and Toho-1 6/24 (25%). Of these, 14/24 (58%) presented more than one genotype for the tested primers. In nine (37%) samples other than E. coli, K. pneumoniae or Proteus spp., the phenotype for ESBL was found and confirmed by PCR. The most sensitive substrate in the approximation test in ESBL positive samples was ceftriaxone (83%). Fifty percent of the samples expressed AmpC were associated with other genes. Intermediate susceptibility to ertapenem was found in 2/41 (5%). Keywords: polymerase chain reaction, infection control, enterobacteriaceae infections |
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| format | Article |
| id | doaj.art-b6dbfc6e2a4d4de8a51676a855eb25a8 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-13T06:22:19Z |
| publishDate | 2011-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-b6dbfc6e2a4d4de8a51676a855eb25a82022-12-21T23:56:49ZengElsevierBrazilian Journal of Infectious Diseases1413-86702011-01-011512833β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, BrazilMarcia Regina G. Vasques0Alexandre Ribeiro Bello1Cristiane da Cruz Lamas2Juarez Correa3José Augusto Adler Pereira4Especialist, Hospitalar Infection Control and Prevention; Master's degree, Medical Microbiology; Nurse, Hospitalar Infection Control, Instituto Nacional de Cardiologia – RJ; Correspondence to: Vasques Rua Thompson Flores, 148, Méier, Rio de Janeiro – RJ.PhD, Parasitology; Adjunct Professor, Department of Microbiology, Immunology and Parasitology (DMIP), Universidade do Estado do Rio de Janeiro – UERJ; Professor, Post-Graduation Programme, UERJMD, Infectologist, Infection Control, Instituto Nacional de Cardiologia; Professor, UNIGRANRIOBiochemical Pharmacist; Microbiologist, Laboratório LABOSERV, Rio de JaneiroProfessor, Medical Microbiology; Associate Professor, Department of Microbiology, Immunology and Parasitology (DMIP), UERJ; Professor, Post-Graduation Programme, UERJThere is a high incidence of infections caused by betalactamase-producing Gram-negative microorganisms in Brazil. These organisms are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. The present study was conducted in sentinel rectal swabs from patients admitted to a cardiac surgery hospital in Rio de Janeiro, from January through December 2007, in a consecutive manner. The aim of the study was to characterize the genotype and phenotype of these isolates from colonized patients. Biochemical tests, antimicrobial susceptibility tests, a confirmatory test for the expression of extended spectrum betalactamase (ESBL) production and polymerase chain reaction for the blaTEM, blaSHV, CTX-M1, Toho-1 and AmpC genes were performed at the University Hospital of Universidade do Estado do Rio de Janeiro (UERJ). The most frequently isolated bacteria were Escherichia coli 9/41 (21.95%) and Klebsiella pneumoniae 14/41 (34.1%). In 24/41 (58%), the ESBL genotype was confirmed. The most prevalent genes in samples that expressed ESBL were blaTEM 13/24 (54%), AmpC 12/24 (50%), blaSHV 6/24 (25%), CTX-M1 7/24 (29%), and Toho-1 6/24 (25%). Of these, 14/24 (58%) presented more than one genotype for the tested primers. In nine (37%) samples other than E. coli, K. pneumoniae or Proteus spp., the phenotype for ESBL was found and confirmed by PCR. The most sensitive substrate in the approximation test in ESBL positive samples was ceftriaxone (83%). Fifty percent of the samples expressed AmpC were associated with other genes. Intermediate susceptibility to ertapenem was found in 2/41 (5%). Keywords: polymerase chain reaction, infection control, enterobacteriaceae infectionshttp://www.sciencedirect.com/science/article/pii/S1413867011701362 |
| spellingShingle | Marcia Regina G. Vasques Alexandre Ribeiro Bello Cristiane da Cruz Lamas Juarez Correa José Augusto Adler Pereira β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, Brazil Brazilian Journal of Infectious Diseases |
| title | β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, Brazil |
| title_full | β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, Brazil |
| title_fullStr | β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, Brazil |
| title_full_unstemmed | β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, Brazil |
| title_short | β-lactamase producing enterobacteria isolated from surveillance swabs of patients in a Cardiac Intensive Care Unit in Rio de Janeiro, Brazil |
| title_sort | β lactamase producing enterobacteria isolated from surveillance swabs of patients in a cardiac intensive care unit in rio de janeiro brazil |
| url | http://www.sciencedirect.com/science/article/pii/S1413867011701362 |
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