<i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived Macrophage
Envenomation caused by contact with <i>Lonomia obliqua</i> bristles is characterized by pain, an intense systemic proinflammatory reaction and disturbances in the coagulation cascade that can cause severe clinical manifestations and death. However, the role of immune system components in...
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MDPI AG
2021-06-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/7/462 |
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| author | Douglas Souza Oliveira Jean Gabriel de Souza Miryam Paola Alvarez-Flores Priscila S. Cunegundes Carlos DeOcesano-Pereira Aline Maia Lobba Renata N. Gomes Ana Marisa Chudzinski-Tavassi |
| author_facet | Douglas Souza Oliveira Jean Gabriel de Souza Miryam Paola Alvarez-Flores Priscila S. Cunegundes Carlos DeOcesano-Pereira Aline Maia Lobba Renata N. Gomes Ana Marisa Chudzinski-Tavassi |
| author_sort | Douglas Souza Oliveira |
| collection | DOAJ |
| description | Envenomation caused by contact with <i>Lonomia obliqua</i> bristles is characterized by pain, an intense systemic proinflammatory reaction and disturbances in the coagulation cascade that can cause severe clinical manifestations and death. However, the role of immune system components in these effects is still poorly understood. In this study, we evaluated the cytotoxic effect of <i>L. obliqua</i> venom on THP-1-derived macrophages and its ability to modulate inflammatory markers, as well as the cytokine and chemokine release profile. Our results show that <i>L. obliqua</i> venom is able to directly exert a potent pro-inflammatory reaction in macrophages, characterized by the activation of the NF-κB transcription factor pathway, the expression of CD80 and CD83, and the release of pro-inflammatory mediators such as TNF-α, IL-1β, IL-6, IL-8 and CXCL10. These results suggest that macrophages can play an important role during the orchestration of the inflammatory response present in envenomation caused by <i>Lonomia obliqua</i> caterpillars. |
| first_indexed | 2024-03-10T09:54:04Z |
| format | Article |
| id | doaj.art-b6e2c8f26c9340dab8fe56388291f40b |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T09:54:04Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b6e2c8f26c9340dab8fe56388291f40b2023-11-22T02:31:55ZengMDPI AGToxins2072-66512021-06-0113746210.3390/toxins13070462<i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived MacrophageDouglas Souza Oliveira0Jean Gabriel de Souza1Miryam Paola Alvarez-Flores2Priscila S. Cunegundes3Carlos DeOcesano-Pereira4Aline Maia Lobba5Renata N. Gomes6Ana Marisa Chudzinski-Tavassi7Centre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilCentre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilCentre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilCentre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilCentre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilCentre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilCentre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilCentre of Excellence in New Target Discovery (CENTD), Butantan Institute, Butantã 05503-900, SP, BrazilEnvenomation caused by contact with <i>Lonomia obliqua</i> bristles is characterized by pain, an intense systemic proinflammatory reaction and disturbances in the coagulation cascade that can cause severe clinical manifestations and death. However, the role of immune system components in these effects is still poorly understood. In this study, we evaluated the cytotoxic effect of <i>L. obliqua</i> venom on THP-1-derived macrophages and its ability to modulate inflammatory markers, as well as the cytokine and chemokine release profile. Our results show that <i>L. obliqua</i> venom is able to directly exert a potent pro-inflammatory reaction in macrophages, characterized by the activation of the NF-κB transcription factor pathway, the expression of CD80 and CD83, and the release of pro-inflammatory mediators such as TNF-α, IL-1β, IL-6, IL-8 and CXCL10. These results suggest that macrophages can play an important role during the orchestration of the inflammatory response present in envenomation caused by <i>Lonomia obliqua</i> caterpillars.https://www.mdpi.com/2072-6651/13/7/462<i>Lonomia obliqua</i>inflammationmacrophage activation |
| spellingShingle | Douglas Souza Oliveira Jean Gabriel de Souza Miryam Paola Alvarez-Flores Priscila S. Cunegundes Carlos DeOcesano-Pereira Aline Maia Lobba Renata N. Gomes Ana Marisa Chudzinski-Tavassi <i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived Macrophage Toxins <i>Lonomia obliqua</i> inflammation macrophage activation |
| title | <i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived Macrophage |
| title_full | <i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived Macrophage |
| title_fullStr | <i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived Macrophage |
| title_full_unstemmed | <i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived Macrophage |
| title_short | <i>Lonomia obliqua</i> Venom Induces NF-κB Activation and a Pro-Inflammatory Profile in THP-1-Derived Macrophage |
| title_sort | i lonomia obliqua i venom induces nf κb activation and a pro inflammatory profile in thp 1 derived macrophage |
| topic | <i>Lonomia obliqua</i> inflammation macrophage activation |
| url | https://www.mdpi.com/2072-6651/13/7/462 |
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