Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.843887/full |
| _version_ | 1819121459192135680 |
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| author | Xiutao Liu Guang Zhao Shengjie Sun Chuanle Fan Chuanle Fan Xinjun Feng Peng Xiong |
| author_facet | Xiutao Liu Guang Zhao Shengjie Sun Chuanle Fan Chuanle Fan Xinjun Feng Peng Xiong |
| author_sort | Xiutao Liu |
| collection | DOAJ |
| description | Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environment pollution, succinic acid biosynthesis has drawn increasing attention as a viable, more environmentally friendly alternative. To date, several metabolic engineering approaches have been utilized for constructing and optimizing succinic acid cell factories. In this review, different succinic acid biosynthesis pathways are summarized, with a focus on the key enzymes and metabolic engineering approaches, which mainly include redirecting carbon flux, balancing NADH/NAD+ ratios, and optimizing CO2 supplementation. Finally, future perspectives on the microbial production of succinic acid are discussed. |
| first_indexed | 2024-12-22T06:36:54Z |
| format | Article |
| id | doaj.art-b6e3fa91b9584b90bde98d285a4fc546 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-22T06:36:54Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b6e3fa91b9584b90bde98d285a4fc5462022-12-21T18:35:34ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.843887843887Biosynthetic Pathway and Metabolic Engineering of Succinic AcidXiutao Liu0Guang Zhao1Shengjie Sun2Chuanle Fan3Chuanle Fan4Xinjun Feng5Peng Xiong6School of Life Sciences and Medicine, Shandong University of Technology, Zibo, ChinaState Key Lab of Microbial Technology, Shandong University, Qingdao, ChinaSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaSchool of Chemical Engineering, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, ChinaSuccinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environment pollution, succinic acid biosynthesis has drawn increasing attention as a viable, more environmentally friendly alternative. To date, several metabolic engineering approaches have been utilized for constructing and optimizing succinic acid cell factories. In this review, different succinic acid biosynthesis pathways are summarized, with a focus on the key enzymes and metabolic engineering approaches, which mainly include redirecting carbon flux, balancing NADH/NAD+ ratios, and optimizing CO2 supplementation. Finally, future perspectives on the microbial production of succinic acid are discussed.https://www.frontiersin.org/articles/10.3389/fbioe.2022.843887/fullsuccinic acidbiosynthesis pathwaysmetabolic engineeringCO2 fixationNADH/NAD + ratio |
| spellingShingle | Xiutao Liu Guang Zhao Shengjie Sun Chuanle Fan Chuanle Fan Xinjun Feng Peng Xiong Biosynthetic Pathway and Metabolic Engineering of Succinic Acid Frontiers in Bioengineering and Biotechnology succinic acid biosynthesis pathways metabolic engineering CO2 fixation NADH/NAD + ratio |
| title | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
| title_full | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
| title_fullStr | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
| title_full_unstemmed | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
| title_short | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
| title_sort | biosynthetic pathway and metabolic engineering of succinic acid |
| topic | succinic acid biosynthesis pathways metabolic engineering CO2 fixation NADH/NAD + ratio |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.843887/full |
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