Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway
Objective(s): This study was conducted to examine the therapeutic effects of lavender oil (LO) against oxygen-glucose deprivation (OGD)-induced injury in vitro model.Materials and Methods: In this study, the OGD model was induced in the H9C2 cell line, and then the cells were treated with LO (10, 10...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-02-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_19589_c2fea7e8ca66e5c324d67ab83a149042.pdf |
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| author | Shaghayegh Askarian-Amiri Hoda Fotovat eskandari Fatemeh Ramezani Gelare Vahabzadeh Nahid Aboutaleb |
| author_facet | Shaghayegh Askarian-Amiri Hoda Fotovat eskandari Fatemeh Ramezani Gelare Vahabzadeh Nahid Aboutaleb |
| author_sort | Shaghayegh Askarian-Amiri |
| collection | DOAJ |
| description | Objective(s): This study was conducted to examine the therapeutic effects of lavender oil (LO) against oxygen-glucose deprivation (OGD)-induced injury in vitro model.Materials and Methods: In this study, the OGD model was induced in the H9C2 cell line, and then the cells were treated with LO (10, 100, 1000, and 10000 μg/ml). The anti-inflammatory activity of LO (Jak2/Stat3) was evaluated by immunocytochemical assay. Likewise, the p-ERK/ERK level was measured by western blotting.Results: Compared with only the OGD-induced injury model, cell survival increased after treatment with LO. Our results showed that 100 μg/ml of LO significantly decreased the expression of Jak2/Stat3 and the apoptotic activity 72 hr after reperfusion compared with the control group. Likewise, significant increases were observed in p-ERK/ERK in LO-treated groups. Conclusion: Collectively, these findings confirm that LO can be a good candidate to reduce OGD-induced injury in the H9C2 cell line through targeting Jak2/Stat3 and ERK pathways. |
| first_indexed | 2024-04-12T22:42:53Z |
| format | Article |
| id | doaj.art-b6e4e0e00dc94735aa64225438a12aa5 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T22:42:53Z |
| publishDate | 2022-02-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-b6e4e0e00dc94735aa64225438a12aa52022-12-22T03:13:39ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-02-0125226326710.22038/ijbms.2022.54751.1228019589Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathwayShaghayegh Askarian-Amiri0Hoda Fotovat eskandari1Fatemeh Ramezani2Gelare Vahabzadeh3Nahid Aboutaleb4Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 71 Avenue des Martyrs, 38000 Grenoble, FrancePhysiology Research Center, Iran University of Medical Sciences, Tehran, IranPhysiology Research Center, Iran University of Medical Sciences, Tehran, IranDepartment of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, IranPhysiology Research Center, Iran University of Medical Sciences, Tehran, IranObjective(s): This study was conducted to examine the therapeutic effects of lavender oil (LO) against oxygen-glucose deprivation (OGD)-induced injury in vitro model.Materials and Methods: In this study, the OGD model was induced in the H9C2 cell line, and then the cells were treated with LO (10, 100, 1000, and 10000 μg/ml). The anti-inflammatory activity of LO (Jak2/Stat3) was evaluated by immunocytochemical assay. Likewise, the p-ERK/ERK level was measured by western blotting.Results: Compared with only the OGD-induced injury model, cell survival increased after treatment with LO. Our results showed that 100 μg/ml of LO significantly decreased the expression of Jak2/Stat3 and the apoptotic activity 72 hr after reperfusion compared with the control group. Likewise, significant increases were observed in p-ERK/ERK in LO-treated groups. Conclusion: Collectively, these findings confirm that LO can be a good candidate to reduce OGD-induced injury in the H9C2 cell line through targeting Jak2/Stat3 and ERK pathways.https://ijbms.mums.ac.ir/article_19589_c2fea7e8ca66e5c324d67ab83a149042.pdfh9c2 cellsinflammationischemia/reperfusion injuryjak2 / stat3lavender oilp-erk / erk |
| spellingShingle | Shaghayegh Askarian-Amiri Hoda Fotovat eskandari Fatemeh Ramezani Gelare Vahabzadeh Nahid Aboutaleb Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway Iranian Journal of Basic Medical Sciences h9c2 cells inflammation ischemia/reperfusion injury jak2 / stat3 lavender oil p-erk / erk |
| title | Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway |
| title_full | Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway |
| title_fullStr | Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway |
| title_full_unstemmed | Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway |
| title_short | Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway |
| title_sort | lavender protects h9c2 cardiomyocytes against oxygen glucose deprivation ogd induced injury via targeting the jak2 stat3 pathway |
| topic | h9c2 cells inflammation ischemia/reperfusion injury jak2 / stat3 lavender oil p-erk / erk |
| url | https://ijbms.mums.ac.ir/article_19589_c2fea7e8ca66e5c324d67ab83a149042.pdf |
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