Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted Nutrients
Soybean roots are colonized and nodulated by multiple strains of compatible nitrogen-fixing rhizobia primarily belonging to the Genus <i>Bradyrhizobium</i>. Motility towards the root and attachment to root hairs are key determinants of competitive colonization and subsequent nodulation....
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Nitrogen |
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| Online Access: | https://www.mdpi.com/2504-3129/2/4/31 |
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| author | Armaan Kaur Sandhu Senthil Subramanian Volker S. Brözel |
| author_facet | Armaan Kaur Sandhu Senthil Subramanian Volker S. Brözel |
| author_sort | Armaan Kaur Sandhu |
| collection | DOAJ |
| description | Soybean roots are colonized and nodulated by multiple strains of compatible nitrogen-fixing rhizobia primarily belonging to the Genus <i>Bradyrhizobium</i>. Motility towards the root and attachment to root hairs are key determinants of competitive colonization and subsequent nodulation. Bacterial surface properties and motility are known to vary with chemical composition of the culture medium, and root adhesion and nodulation occur in a soil environment rather than laboratory medium. We asked whether the nodulation-promoting factors motility, surface hydrophobicity and surface adhesion of <i>Bradyrhizobium</i> are affected by growth in a soil nutrient environment. <i>B. diazoefficiens</i> USDA 110, 126, 3384, and <i>B. elkanii</i> USDA 26 were grown in mineral salt medium with peptone, yeast extract and arabinose (PSY), and in a soil extracted soluble organic matter (SESOM) medium. Surface hydrophobicity was determined by partitioning into hydrocarbon, motility by transition through soft agar, and surface-exposed saccharides by lectin profiling, followed by biofilm formation and soybean root adhesion capacity of populations. SESOM-grown populations were generally less motile and more hydrophobic. They bound fewer lectins than PSY-grown populations, indicating a simpler surface saccharide profile. SESOM populations of USDA 110 did not form detectable biofilm, but showed increased binding to soy roots. Our results indicate that growth in a soil environment impacts surface properties, motility, and subsequent soy root adhesion propensity. Hence, evaluation of <i>Bradyrhizobium</i> for nodulation efficiency should be performed using soil from the specific field where the soybeans are to be planted, rather than laboratory culture media. |
| first_indexed | 2024-03-10T03:25:40Z |
| format | Article |
| id | doaj.art-b6e576187ae641d6a76649b477baf646 |
| institution | Directory Open Access Journal |
| issn | 2504-3129 |
| language | English |
| last_indexed | 2024-03-10T03:25:40Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nitrogen |
| spelling | doaj.art-b6e576187ae641d6a76649b477baf6462023-11-23T09:53:51ZengMDPI AGNitrogen2504-31292021-11-012446147310.3390/nitrogen2040031Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted NutrientsArmaan Kaur Sandhu0Senthil Subramanian1Volker S. Brözel2Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USADepartment of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USADepartment of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USASoybean roots are colonized and nodulated by multiple strains of compatible nitrogen-fixing rhizobia primarily belonging to the Genus <i>Bradyrhizobium</i>. Motility towards the root and attachment to root hairs are key determinants of competitive colonization and subsequent nodulation. Bacterial surface properties and motility are known to vary with chemical composition of the culture medium, and root adhesion and nodulation occur in a soil environment rather than laboratory medium. We asked whether the nodulation-promoting factors motility, surface hydrophobicity and surface adhesion of <i>Bradyrhizobium</i> are affected by growth in a soil nutrient environment. <i>B. diazoefficiens</i> USDA 110, 126, 3384, and <i>B. elkanii</i> USDA 26 were grown in mineral salt medium with peptone, yeast extract and arabinose (PSY), and in a soil extracted soluble organic matter (SESOM) medium. Surface hydrophobicity was determined by partitioning into hydrocarbon, motility by transition through soft agar, and surface-exposed saccharides by lectin profiling, followed by biofilm formation and soybean root adhesion capacity of populations. SESOM-grown populations were generally less motile and more hydrophobic. They bound fewer lectins than PSY-grown populations, indicating a simpler surface saccharide profile. SESOM populations of USDA 110 did not form detectable biofilm, but showed increased binding to soy roots. Our results indicate that growth in a soil environment impacts surface properties, motility, and subsequent soy root adhesion propensity. Hence, evaluation of <i>Bradyrhizobium</i> for nodulation efficiency should be performed using soil from the specific field where the soybeans are to be planted, rather than laboratory culture media.https://www.mdpi.com/2504-3129/2/4/31<i>Bradyrhizobium</i>attachmentrootbiofilmlectinsoybean |
| spellingShingle | Armaan Kaur Sandhu Senthil Subramanian Volker S. Brözel Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted Nutrients Nitrogen <i>Bradyrhizobium</i> attachment root biofilm lectin soybean |
| title | Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted Nutrients |
| title_full | Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted Nutrients |
| title_fullStr | Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted Nutrients |
| title_full_unstemmed | Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted Nutrients |
| title_short | Surface Properties and Adherence of <i>Bradyrhizobium diazoefficiens</i> to <i>Glycine max</i> Roots Are Altered When Grown in Soil Extracted Nutrients |
| title_sort | surface properties and adherence of i bradyrhizobium diazoefficiens i to i glycine max i roots are altered when grown in soil extracted nutrients |
| topic | <i>Bradyrhizobium</i> attachment root biofilm lectin soybean |
| url | https://www.mdpi.com/2504-3129/2/4/31 |
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