| Summary: | Objective: To compare the application value of the fluorescence staining method with KOH wet method in superficial fungal infections of the skin. Methods: One hundred and ninety two outpatient suspected patients of superficial fungal skin infection were collected. Specimens were divided into two parts, and detected by fluorescent staining and KOH wet method at the same time, then the test results were compared. Result: With fluorescent staining method: the positive rate was 73.4% (141/192). In the patients involved with trunk and femoral-genital region, the positive rate was 78.6% (77/98). Whereas in patients involved with hands and feet, positive rate was 75.0% (39/52). As regards to the fingers/toes region, positive rate was 59.5% (25/42). With KOH wet positive method:the positive rate was 53.6% (103/192). The positive rate in the trunk region, in hands and feet and in fingers/toes was 60.2%(59/98), 53.8%(28/52), 38.1% (16/42), respectively. The positive rate of fluorescent fungus staining test was significantly higher than those of KOH wet method (χ2 =16.23, P<0.05). Relative to KOH wet method, the specificity of fluorescent staining method to detect regions of trunk, feet and fingers/toes region were 53.8%, 54.2% and 65.4%, respectively, and the sensitivity was 100%. Conclusion: As for in-experienced technician, fluorescent staining is easy to perform, with a high accuracy. The fungi figures are easily distinguishable even to patients, resulting in high patient compliance. Therefore, it is applicable to clinic work.
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