Determinants of Infection as Genetic Indicators in Cows Mastitis
The resistance to antimicrobial substances severely impacts public health and the abuse of antibiotics leads to antimicrobial resistance (AMR) or the antibiotic "resistome" (Wright, 2007). Bovine mastitis is largely diagnosed in dairy farms and is caused by a variety of pathogens including...
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| Format: | Article |
| Language: | English |
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AcademicPres
2022-05-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
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| Online Access: | https://journals.usamvcluj.ro/index.php/veterinary/article/view/14387 |
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| author | Bianca Cornelia LUNGU Ovidiu Ionuț GEORGESCU Beatrice Ana-Maria TUDOR Calin MIRCU Paul BARROW Hortensja Łucja BRZÓSKA Ioan HUȚU |
| author_facet | Bianca Cornelia LUNGU Ovidiu Ionuț GEORGESCU Beatrice Ana-Maria TUDOR Calin MIRCU Paul BARROW Hortensja Łucja BRZÓSKA Ioan HUȚU |
| author_sort | Bianca Cornelia LUNGU |
| collection | DOAJ |
| description | The resistance to antimicrobial substances severely impacts public health and the abuse of antibiotics leads to antimicrobial resistance (AMR) or the antibiotic "resistome" (Wright, 2007). Bovine mastitis is largely diagnosed in dairy farms and is caused by a variety of pathogens including Streptococcus spp., Staphylococcus spp. and Escherichia coli. AMR gene expression testing in bacteria involved in mastitis in dairy cows was performed. Milk samples were subjected to the California Mastitis Test. Positive samples were transferred using eSwab, cultured on Columbia blood agar and on MacConkey agar. The Qiagen DNeasy kit was used for DNA extraction and qPCRs were run using an Agilent thermocycler. In most of the samples tested (n = 42, from three different lactating farms), the presence of ampC (36 out of 42; 85.7%) and blaZ (95.2%), correlated with confirmed resistance to beta-lactam and cephalosporin antibiotics. A variable presence of other tested AMR genes was detected, including ermB, resistance to lincosamide, macrolide (35.7%), ermC (28.6%), erythromycin resistance, mecA,methicillinresistance (42.9%), and tetK, tetracyclineresistance (78.6%). The phenomenon of antimicrobial resistance is present in dairy farms in West Romania. Multiple AMR genes were detected intested samples, with the highest resistance observed to beta-lactam antibiotics and cephalosporins. |
| first_indexed | 2024-12-12T05:45:09Z |
| format | Article |
| id | doaj.art-b6ea412bd1b2451685a7281c36eec692 |
| institution | Directory Open Access Journal |
| issn | 1843-5270 1843-5378 |
| language | English |
| last_indexed | 2024-12-12T05:45:09Z |
| publishDate | 2022-05-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| spelling | doaj.art-b6ea412bd1b2451685a7281c36eec6922022-12-22T00:35:49ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine1843-52701843-53782022-05-01791111710.15835/buasvmcn-vm:2021.003611716Determinants of Infection as Genetic Indicators in Cows MastitisBianca Cornelia LUNGU0Ovidiu Ionuț GEORGESCU1Beatrice Ana-Maria TUDOR2Calin MIRCU3Paul BARROW4Hortensja Łucja BRZÓSKA5Ioan HUȚU6Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine” King Michael I of Romania” from TimișoaraBanat University of Agricultural Sciences and Veterinary Medicine” King Michael I of Romania” from TimișoaraBanat University of Agricultural Sciences and Veterinary Medicine” King Michael I of Romania” from TimișoaraBanat University of Agricultural Sciences and Veterinary Medicine” King Michael I of Romania” from TimișoaraSchool of Veterinary Medicine and Science, University of Surrey, Daphne Jackson Road, Guildford, Surrey GU2 7AL, United KingdomBanat University of Agricultural Sciences and Veterinary Medicine” King Michael I of Romania” from TimișoaraBanat University of Agricultural Sciences and Veterinary Medicine” King Michael I of Romania” from TimișoaraThe resistance to antimicrobial substances severely impacts public health and the abuse of antibiotics leads to antimicrobial resistance (AMR) or the antibiotic "resistome" (Wright, 2007). Bovine mastitis is largely diagnosed in dairy farms and is caused by a variety of pathogens including Streptococcus spp., Staphylococcus spp. and Escherichia coli. AMR gene expression testing in bacteria involved in mastitis in dairy cows was performed. Milk samples were subjected to the California Mastitis Test. Positive samples were transferred using eSwab, cultured on Columbia blood agar and on MacConkey agar. The Qiagen DNeasy kit was used for DNA extraction and qPCRs were run using an Agilent thermocycler. In most of the samples tested (n = 42, from three different lactating farms), the presence of ampC (36 out of 42; 85.7%) and blaZ (95.2%), correlated with confirmed resistance to beta-lactam and cephalosporin antibiotics. A variable presence of other tested AMR genes was detected, including ermB, resistance to lincosamide, macrolide (35.7%), ermC (28.6%), erythromycin resistance, mecA,methicillinresistance (42.9%), and tetK, tetracyclineresistance (78.6%). The phenomenon of antimicrobial resistance is present in dairy farms in West Romania. Multiple AMR genes were detected intested samples, with the highest resistance observed to beta-lactam antibiotics and cephalosporins.https://journals.usamvcluj.ro/index.php/veterinary/article/view/14387antimicrobial resistancegene expressionmastitis |
| spellingShingle | Bianca Cornelia LUNGU Ovidiu Ionuț GEORGESCU Beatrice Ana-Maria TUDOR Calin MIRCU Paul BARROW Hortensja Łucja BRZÓSKA Ioan HUȚU Determinants of Infection as Genetic Indicators in Cows Mastitis Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine antimicrobial resistance gene expression mastitis |
| title | Determinants of Infection as Genetic Indicators in Cows Mastitis |
| title_full | Determinants of Infection as Genetic Indicators in Cows Mastitis |
| title_fullStr | Determinants of Infection as Genetic Indicators in Cows Mastitis |
| title_full_unstemmed | Determinants of Infection as Genetic Indicators in Cows Mastitis |
| title_short | Determinants of Infection as Genetic Indicators in Cows Mastitis |
| title_sort | determinants of infection as genetic indicators in cows mastitis |
| topic | antimicrobial resistance gene expression mastitis |
| url | https://journals.usamvcluj.ro/index.php/veterinary/article/view/14387 |
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