Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure
BackgroundIn recent times, there has been an increase in the incidence of type 2 diabetes mellitus (T2DM) particularly in children. Adipocyte dysfunction provide a critical link between obesity and insulin resistance resulting in diabetes outcome. Further, environmental chemical exposure during earl...
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| Format: | Article |
| Language: | English |
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Korean Diabetes Association
2016-08-01
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| Series: | Diabetes & Metabolism Journal |
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| Online Access: | https://e-dmj.org/Synapse/Data/PDFData/2004DMJ/dmj-40-494.pdf |
| _version_ | 1828774830167556096 |
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| author | Worlanyo E. Gato Daniel A. Hunter Shamaya L. Whitby Christopher A. Mays Wilson Yau |
| author_facet | Worlanyo E. Gato Daniel A. Hunter Shamaya L. Whitby Christopher A. Mays Wilson Yau |
| author_sort | Worlanyo E. Gato |
| collection | DOAJ |
| description | BackgroundIn recent times, there has been an increase in the incidence of type 2 diabetes mellitus (T2DM) particularly in children. Adipocyte dysfunction provide a critical link between obesity and insulin resistance resulting in diabetes outcome. Further, environmental chemical exposure during early years of life might be a significant contributing factor to the increase in the incidence of T2DM. This study tests the idea that exposure to environmental contaminants (2-aminoanthracene [2AA]) in utero will show effects in the adipose tissue (AT) that signify T2DM vulnerability. 2AA is a polycyclic aromatic hydrocarbon found in a variety of products.MethodsTo accomplish the study objective, pregnant dams were fed various amounts of 2AA adulterated diets from gestation through postnatal period. The neonates and older offspring were analyzed for diabetic-like genes in the ATs and analysis of serum glucose. Furthermore, weight monitoring, histopathology and immunohistochemical (IHC) staining for CD68 in AT, adipocyte size determination and adiponectin amounts in serum were undertaken.ResultsUp-regulation of adiponectin and interleukin-6 genes were noted in the pups and older rats. Combination of intrauterine 2AA toxicity with moderate high fat diet exhibited gene expression patterns similar to those of the neonates. Elevated serum glucose levels were noted in treated groups. IHC of the AT indicated no significant malformations; however, CD68+ cells were greater in the animals treated to 2AA. Similarly, mean sizes of the adipocytes were larger in treated and combined 2AA and moderate high fat animals. Adiponectin was reduced in 2AA groups.ConclusionFrom the preceding, it appears intrauterine 2AA disturbance, when combined with excess fat accumulation will lead to greater risk for the diabetic condition. |
| first_indexed | 2024-12-11T15:29:20Z |
| format | Article |
| id | doaj.art-b6efdb5c7afd41e9b08665e18ba88d7b |
| institution | Directory Open Access Journal |
| issn | 2233-6079 2233-6087 |
| language | English |
| last_indexed | 2024-12-11T15:29:20Z |
| publishDate | 2016-08-01 |
| publisher | Korean Diabetes Association |
| record_format | Article |
| series | Diabetes & Metabolism Journal |
| spelling | doaj.art-b6efdb5c7afd41e9b08665e18ba88d7b2022-12-22T01:00:07ZengKorean Diabetes AssociationDiabetes & Metabolism Journal2233-60792233-60872016-08-0140649450810.4093/dmj.2016.40.6.494Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons ExposureWorlanyo E. Gato0Daniel A. Hunter1Shamaya L. Whitby2Christopher A. Mays3Wilson Yau4Department of Chemistry, Georgia Southern University, Statesboro, GA, USA.Department of Chemistry, Georgia Southern University, Statesboro, GA, USA.Department of Chemistry, Georgia Southern University, Statesboro, GA, USA.Department of Chemistry, Georgia Southern University, Statesboro, GA, USA.Department of Pathology, University of Georgia College of Veterinary Medicine, Athens, GA, USA.BackgroundIn recent times, there has been an increase in the incidence of type 2 diabetes mellitus (T2DM) particularly in children. Adipocyte dysfunction provide a critical link between obesity and insulin resistance resulting in diabetes outcome. Further, environmental chemical exposure during early years of life might be a significant contributing factor to the increase in the incidence of T2DM. This study tests the idea that exposure to environmental contaminants (2-aminoanthracene [2AA]) in utero will show effects in the adipose tissue (AT) that signify T2DM vulnerability. 2AA is a polycyclic aromatic hydrocarbon found in a variety of products.MethodsTo accomplish the study objective, pregnant dams were fed various amounts of 2AA adulterated diets from gestation through postnatal period. The neonates and older offspring were analyzed for diabetic-like genes in the ATs and analysis of serum glucose. Furthermore, weight monitoring, histopathology and immunohistochemical (IHC) staining for CD68 in AT, adipocyte size determination and adiponectin amounts in serum were undertaken.ResultsUp-regulation of adiponectin and interleukin-6 genes were noted in the pups and older rats. Combination of intrauterine 2AA toxicity with moderate high fat diet exhibited gene expression patterns similar to those of the neonates. Elevated serum glucose levels were noted in treated groups. IHC of the AT indicated no significant malformations; however, CD68+ cells were greater in the animals treated to 2AA. Similarly, mean sizes of the adipocytes were larger in treated and combined 2AA and moderate high fat animals. Adiponectin was reduced in 2AA groups.ConclusionFrom the preceding, it appears intrauterine 2AA disturbance, when combined with excess fat accumulation will lead to greater risk for the diabetic condition.https://e-dmj.org/Synapse/Data/PDFData/2004DMJ/dmj-40-494.pdfAdipocytesAdiponectinDiabetesGene expressionInterleukin-6Serum glucose2-Anthramine |
| spellingShingle | Worlanyo E. Gato Daniel A. Hunter Shamaya L. Whitby Christopher A. Mays Wilson Yau Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure Diabetes & Metabolism Journal Adipocytes Adiponectin Diabetes Gene expression Interleukin-6 Serum glucose 2-Anthramine |
| title | Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure |
| title_full | Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure |
| title_fullStr | Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure |
| title_full_unstemmed | Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure |
| title_short | Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure |
| title_sort | investigating susceptibility to diabetes using features of the adipose tissue in response to in utero polycyclic aromatic hydrocarbons exposure |
| topic | Adipocytes Adiponectin Diabetes Gene expression Interleukin-6 Serum glucose 2-Anthramine |
| url | https://e-dmj.org/Synapse/Data/PDFData/2004DMJ/dmj-40-494.pdf |
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