Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens
Summary: Background: The pathogenicity of Staphylococcus aureus is based on the production of various virulence factors. The frequency of these factors can markedly differ according to the geographical region. Materials and methods: In this study, we investigated the prevalence of two frequent isof...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-05-01
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| Series: | Journal of Infection and Public Health |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034114000082 |
| _version_ | 1818132511107055616 |
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| author | Roohollah Zarei Koosha Abbas Ali Imani Fooladi Hamideh Mahmoodzadeh Hosseini Elnaz Mehdizadeh Aghdam |
| author_facet | Roohollah Zarei Koosha Abbas Ali Imani Fooladi Hamideh Mahmoodzadeh Hosseini Elnaz Mehdizadeh Aghdam |
| author_sort | Roohollah Zarei Koosha |
| collection | DOAJ |
| description | Summary: Background: The pathogenicity of Staphylococcus aureus is based on the production of various virulence factors. The frequency of these factors can markedly differ according to the geographical region. Materials and methods: In this study, we investigated the prevalence of two frequent isoforms of exfoliative toxins and mecA genes using PCR in 197 clinical isolates obtained from clinical samples during the years 2011 and 2012. The samples were obtained from an educational hospital in northern Tehran, Iran. In addition, the antibiotic susceptibility pattern was studied for each isolate using the disc diffusion method. Results: In this study, 186 (94.4%), 15 (7.6%) and 172 (86.3%) of the 197 isolates expressed the eta, etb and mecA genes, respectively. In addition, 164 (88.2%) and 12 (80%) strains, which harbored the eta and etb genes, respectively, were MRSA (methicillin resistant S. aureus). Furthermore, the prevalence of the mecA, eta and etb genes was higher among the wound samples (61.2%, 55.8% and 6.09%, respectively). We observed high rates of MDR (multi drug resistance) among our isolates. A significant correlation was detected between the presence of the mecA gene and the resistance to oxacillin, gentamicin, kanamycin, erythromycin, tetracycline, cotrimoxazole, clindamycin, and cephazolin as well as the multi-drug resistant property (P < 0.05). In addition to penicillin, the MDR properties and resistances to the tested antibiotics in the etb-positive strains were significantly lower compared to the etb-negative strains (P < 0.05). Conclusions: The prevalence of the eta, etb and mecA genes might be due to the specific geographic region. Keywords: Exfoliative toxin, Staphylococcus aureus, Multi-drug resistance, Clinical isolate |
| first_indexed | 2024-12-11T08:37:59Z |
| format | Article |
| id | doaj.art-b6f06ab176d44ca2bee3d7763b5647de |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-12-11T08:37:59Z |
| publishDate | 2014-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-b6f06ab176d44ca2bee3d7763b5647de2022-12-22T01:14:18ZengElsevierJournal of Infection and Public Health1876-03412014-05-0173177185Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimensRoohollah Zarei Koosha0Abbas Ali Imani Fooladi1Hamideh Mahmoodzadeh Hosseini2Elnaz Mehdizadeh Aghdam3Biology Department, Islamic Azad University of Jahrom Branch, Jahrom, IranApplied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Corresponding author. Tel.: +98 2188068924; fax: +98 2188068924.Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranSummary: Background: The pathogenicity of Staphylococcus aureus is based on the production of various virulence factors. The frequency of these factors can markedly differ according to the geographical region. Materials and methods: In this study, we investigated the prevalence of two frequent isoforms of exfoliative toxins and mecA genes using PCR in 197 clinical isolates obtained from clinical samples during the years 2011 and 2012. The samples were obtained from an educational hospital in northern Tehran, Iran. In addition, the antibiotic susceptibility pattern was studied for each isolate using the disc diffusion method. Results: In this study, 186 (94.4%), 15 (7.6%) and 172 (86.3%) of the 197 isolates expressed the eta, etb and mecA genes, respectively. In addition, 164 (88.2%) and 12 (80%) strains, which harbored the eta and etb genes, respectively, were MRSA (methicillin resistant S. aureus). Furthermore, the prevalence of the mecA, eta and etb genes was higher among the wound samples (61.2%, 55.8% and 6.09%, respectively). We observed high rates of MDR (multi drug resistance) among our isolates. A significant correlation was detected between the presence of the mecA gene and the resistance to oxacillin, gentamicin, kanamycin, erythromycin, tetracycline, cotrimoxazole, clindamycin, and cephazolin as well as the multi-drug resistant property (P < 0.05). In addition to penicillin, the MDR properties and resistances to the tested antibiotics in the etb-positive strains were significantly lower compared to the etb-negative strains (P < 0.05). Conclusions: The prevalence of the eta, etb and mecA genes might be due to the specific geographic region. Keywords: Exfoliative toxin, Staphylococcus aureus, Multi-drug resistance, Clinical isolatehttp://www.sciencedirect.com/science/article/pii/S1876034114000082 |
| spellingShingle | Roohollah Zarei Koosha Abbas Ali Imani Fooladi Hamideh Mahmoodzadeh Hosseini Elnaz Mehdizadeh Aghdam Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens Journal of Infection and Public Health |
| title | Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens |
| title_full | Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens |
| title_fullStr | Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens |
| title_full_unstemmed | Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens |
| title_short | Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens |
| title_sort | prevalence of exfoliative toxin a and b genes in staphylococcus aureus isolated from clinical specimens |
| url | http://www.sciencedirect.com/science/article/pii/S1876034114000082 |
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