Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88
Introduction: The aim of the experiment was to establish the enterotoxigenic Escherichia coli K88 (ETEC K88)-induced BALB/c mouse duodenum inflammation model. Material and Methods: Mice were administered different concentrations of E. coli K88 (1.0 × 107-109 CFU/mL) for 3 d by means of an esophageal...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2016-03-01
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| Series: | Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/jvetres-2016-0004 |
| _version_ | 1818651621798707200 |
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| author | Wang Kai Qi Yu Yi Shushuai Pei Zhihua Pan Na Hu Guixue |
| author_facet | Wang Kai Qi Yu Yi Shushuai Pei Zhihua Pan Na Hu Guixue |
| author_sort | Wang Kai |
| collection | DOAJ |
| description | Introduction: The aim of the experiment was to establish the enterotoxigenic Escherichia coli K88 (ETEC K88)-induced BALB/c mouse duodenum inflammation model. Material and Methods: Mice were administered different concentrations of E. coli K88 (1.0 × 107-109 CFU/mL) for 3 d by means of an esophageal catheter. Results: The results showed that the treated group expressed several significant clinical symptoms, such as reduced dietary demands and weight loss, an increased presence of IL-1α, TNF-α, and MPO in the peripheral blood, and some pathological changes in the duodenum. On the 6th-8th days, the body weight of the mice was the lowest. On the 8th day, there were significant differences in IL-1α, TNF-α, and MPO levels compared to the control group (P < 0.05), the gap between the duodenum mucous layer and the muscular layer had widened, the number of goblet cells was increased, and the inflammatory infiltrate and inflammation changes in the lamina propria and the mucous layer were the most obvious. Conclusion: The duodenum inflammation was the most severe on day 8; thus, the model was successfully established. In addition, varying concentrations of ETEC K88 did not significantly influence the duodenum inflammation (P > 0.05). |
| first_indexed | 2024-12-17T02:09:02Z |
| format | Article |
| id | doaj.art-b6f200d3eb84408aa9e6a8d400381a18 |
| institution | Directory Open Access Journal |
| issn | 2450-8608 |
| language | English |
| last_indexed | 2024-12-17T02:09:02Z |
| publishDate | 2016-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Veterinary Research |
| spelling | doaj.art-b6f200d3eb84408aa9e6a8d400381a182022-12-21T22:07:37ZengSciendoJournal of Veterinary Research2450-86082016-03-01601192310.1515/jvetres-2016-0004jvetres-2016-0004Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88Wang Kai0Qi Yu1Yi Shushuai2Pei Zhihua3Pan Na4Hu Guixue5College of Animal Science and Technology, Jilin Agricultural University, Xincheng, Changchun 130118, People’s Republic of ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Xincheng, Changchun 130118, People’s Republic of ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Xincheng, Changchun 130118, People’s Republic of ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Xincheng, Changchun 130118, People’s Republic of ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Xincheng, Changchun 130118, People’s Republic of ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Xincheng, Changchun 130118, People’s Republic of ChinaIntroduction: The aim of the experiment was to establish the enterotoxigenic Escherichia coli K88 (ETEC K88)-induced BALB/c mouse duodenum inflammation model. Material and Methods: Mice were administered different concentrations of E. coli K88 (1.0 × 107-109 CFU/mL) for 3 d by means of an esophageal catheter. Results: The results showed that the treated group expressed several significant clinical symptoms, such as reduced dietary demands and weight loss, an increased presence of IL-1α, TNF-α, and MPO in the peripheral blood, and some pathological changes in the duodenum. On the 6th-8th days, the body weight of the mice was the lowest. On the 8th day, there were significant differences in IL-1α, TNF-α, and MPO levels compared to the control group (P < 0.05), the gap between the duodenum mucous layer and the muscular layer had widened, the number of goblet cells was increased, and the inflammatory infiltrate and inflammation changes in the lamina propria and the mucous layer were the most obvious. Conclusion: The duodenum inflammation was the most severe on day 8; thus, the model was successfully established. In addition, varying concentrations of ETEC K88 did not significantly influence the duodenum inflammation (P > 0.05).https://doi.org/10.1515/jvetres-2016-0004mouseduodenumenterotoxigenic escherichia coliinflammation model |
| spellingShingle | Wang Kai Qi Yu Yi Shushuai Pei Zhihua Pan Na Hu Guixue Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88 Journal of Veterinary Research mouse duodenum enterotoxigenic escherichia coli inflammation model |
| title | Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88 |
| title_full | Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88 |
| title_fullStr | Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88 |
| title_full_unstemmed | Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88 |
| title_short | Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88 |
| title_sort | mouse duodenum as a model of inflammation induced by enterotoxigenic escherichia coli k88 |
| topic | mouse duodenum enterotoxigenic escherichia coli inflammation model |
| url | https://doi.org/10.1515/jvetres-2016-0004 |
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