High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples
Acoustic ejection mass spectrometry is a novel high-throughput analytical technology that delivers high reproducibility without carryover observed. It eliminates the chromatography step used to separate analytes from matrix components. Fully-automated liquid–liquid extraction is widely used for samp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/11/11/789 |
| _version_ | 1797509392331964416 |
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| author | Yuzhu Guo Michael Forbush Thomas R. Covey Lucien Ghislain Chang Liu |
| author_facet | Yuzhu Guo Michael Forbush Thomas R. Covey Lucien Ghislain Chang Liu |
| author_sort | Yuzhu Guo |
| collection | DOAJ |
| description | Acoustic ejection mass spectrometry is a novel high-throughput analytical technology that delivers high reproducibility without carryover observed. It eliminates the chromatography step used to separate analytes from matrix components. Fully-automated liquid–liquid extraction is widely used for sample cleanup, especially in high-throughput applications. We introduce a workflow for direct AEMS analysis from phase-separated liquid samples and explore high-throughput analysis from complex matrices. We demonstrate the quantitative determination of fentanyl from urine using this two-phase AEMS approach, with a LOD lower than 1 ng/mL, quantitation precision of 15%, and accuracy better than ±10% over the range of evaluation (1–100 ng/mL). This workflow offers simplified sample preparation and higher analytical throughput for some bioanalytical applications, in comparison to an LC-MS based approach. |
| first_indexed | 2024-03-10T05:17:03Z |
| format | Article |
| id | doaj.art-b6f291e4dcb24e2aa3047370fceb9c1c |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T05:17:03Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-b6f291e4dcb24e2aa3047370fceb9c1c2023-11-23T00:21:34ZengMDPI AGMetabolites2218-19892021-11-01111178910.3390/metabo11110789High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid SamplesYuzhu Guo0Michael Forbush1Thomas R. Covey2Lucien Ghislain3Chang Liu4SCIEX, 71 Four Valley Drive, Concord, ON L4K 4V8, CanadaBeckman Coulter Life Sciences, 170 Rose Orchard Way, San Jose, CA 95134, USASCIEX, 71 Four Valley Drive, Concord, ON L4K 4V8, CanadaBeckman Coulter Life Sciences, 170 Rose Orchard Way, San Jose, CA 95134, USASCIEX, 71 Four Valley Drive, Concord, ON L4K 4V8, CanadaAcoustic ejection mass spectrometry is a novel high-throughput analytical technology that delivers high reproducibility without carryover observed. It eliminates the chromatography step used to separate analytes from matrix components. Fully-automated liquid–liquid extraction is widely used for sample cleanup, especially in high-throughput applications. We introduce a workflow for direct AEMS analysis from phase-separated liquid samples and explore high-throughput analysis from complex matrices. We demonstrate the quantitative determination of fentanyl from urine using this two-phase AEMS approach, with a LOD lower than 1 ng/mL, quantitation precision of 15%, and accuracy better than ±10% over the range of evaluation (1–100 ng/mL). This workflow offers simplified sample preparation and higher analytical throughput for some bioanalytical applications, in comparison to an LC-MS based approach.https://www.mdpi.com/2218-1989/11/11/789acoustic ejection mass spectrometryhigh-throughput analysissample preparationliquid–liquid extraction |
| spellingShingle | Yuzhu Guo Michael Forbush Thomas R. Covey Lucien Ghislain Chang Liu High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples Metabolites acoustic ejection mass spectrometry high-throughput analysis sample preparation liquid–liquid extraction |
| title | High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples |
| title_full | High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples |
| title_fullStr | High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples |
| title_full_unstemmed | High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples |
| title_short | High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples |
| title_sort | high throughput analysis from complex matrices acoustic ejection mass spectrometry from phase separated fluid samples |
| topic | acoustic ejection mass spectrometry high-throughput analysis sample preparation liquid–liquid extraction |
| url | https://www.mdpi.com/2218-1989/11/11/789 |
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