Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities
ABSTRACTMany people in developing countries mainly depend on medicinal plants like Withania somnifera and their products for healthcare. In this study, reflux and maceration extraction methods were applied to investigate total phenolic (TPC) and flavonoid contents (TFC); and antioxidant properties o...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-09-01
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| Series: | International Journal of Food Properties |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2023.2173229 |
| _version_ | 1797202484849016832 |
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| author | Dessie Ezez Natinael Mekonnen Molla Tefera |
| author_facet | Dessie Ezez Natinael Mekonnen Molla Tefera |
| author_sort | Dessie Ezez |
| collection | DOAJ |
| description | ABSTRACTMany people in developing countries mainly depend on medicinal plants like Withania somnifera and their products for healthcare. In this study, reflux and maceration extraction methods were applied to investigate total phenolic (TPC) and flavonoid contents (TFC); and antioxidant properties of Withania somnifera extracts toward DPPH and hydrogen peroxide (H2O2) were studied. Highest TPC was detected with methanol extracts with value of 198.24 ± 1.16 mg GAE/g and TFC of 31.52 ± 0.91 mg QE/g. The minimum amount of TPC was observed in aqueous extract (101.41 ± 1.07 mg GAE/g) and the TFC in ethyl acetate extract 9.72 ± 1.32 mg QE/g. The maximum value of DPPH radical scavenging was estimated in maceration methanol extract 81.98 ± 0.49% and value of H2O2 was obtained in maceration acetone extract 76.18 ± 1.06%. The major identified molecules were Heptasiloxane,1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethyl-(50.04%), and trans-2,4 Dimethylthiane, S, S- dioxide (4.09%) The broad spectrum of antibacterial activity was observed in Escherichia coli reflux methanol extract. In both extraction techniques methanol was the best extracting solvent for phenolic and flavonoid contents as antioxidants as well as antibacterial activities. |
| first_indexed | 2024-03-11T18:20:24Z |
| format | Article |
| id | doaj.art-b6f4558afff04acf9d836368c86b6097 |
| institution | Directory Open Access Journal |
| issn | 1094-2912 1532-2386 |
| language | English |
| last_indexed | 2024-04-24T08:04:11Z |
| publishDate | 2023-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj.art-b6f4558afff04acf9d836368c86b60972024-04-17T13:20:14ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862023-09-0126158159010.1080/10942912.2023.2173229Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activitiesDessie Ezez0Natinael Mekonnen1Molla Tefera2Department of Chemistry, College of Natural Sciences, Arba Minch University, Arba Minch, EthiopiaDepartment of Chemistry, College of Natural Sciences, Arba Minch University, Arba Minch, EthiopiaDepartment of Chemistry, College of Natural Sciences, University of Gondar, Gondar, EthiopiaABSTRACTMany people in developing countries mainly depend on medicinal plants like Withania somnifera and their products for healthcare. In this study, reflux and maceration extraction methods were applied to investigate total phenolic (TPC) and flavonoid contents (TFC); and antioxidant properties of Withania somnifera extracts toward DPPH and hydrogen peroxide (H2O2) were studied. Highest TPC was detected with methanol extracts with value of 198.24 ± 1.16 mg GAE/g and TFC of 31.52 ± 0.91 mg QE/g. The minimum amount of TPC was observed in aqueous extract (101.41 ± 1.07 mg GAE/g) and the TFC in ethyl acetate extract 9.72 ± 1.32 mg QE/g. The maximum value of DPPH radical scavenging was estimated in maceration methanol extract 81.98 ± 0.49% and value of H2O2 was obtained in maceration acetone extract 76.18 ± 1.06%. The major identified molecules were Heptasiloxane,1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethyl-(50.04%), and trans-2,4 Dimethylthiane, S, S- dioxide (4.09%) The broad spectrum of antibacterial activity was observed in Escherichia coli reflux methanol extract. In both extraction techniques methanol was the best extracting solvent for phenolic and flavonoid contents as antioxidants as well as antibacterial activities.https://www.tandfonline.com/doi/10.1080/10942912.2023.2173229AntibacterialAntioxidantFlavonoidpolyphenolWithania somnifera |
| spellingShingle | Dessie Ezez Natinael Mekonnen Molla Tefera Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities International Journal of Food Properties Antibacterial Antioxidant Flavonoid polyphenol Withania somnifera |
| title | Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities |
| title_full | Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities |
| title_fullStr | Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities |
| title_full_unstemmed | Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities |
| title_short | Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities |
| title_sort | phytochemical analysis of withania somnifera leaf extracts by gc ms and evaluating antioxidants and antibacterial activities |
| topic | Antibacterial Antioxidant Flavonoid polyphenol Withania somnifera |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2023.2173229 |
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