Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma
Abstract In this study, Superparamagnetic magnetite nanoparticles (SPMNPs) are used in a new way as direct nanocarrier for Doxorubicin hydrochloride (DOX) via the functionalization of their surface with tri-sodium citrate through ligand exchange to conjugate DOX with imine bond to form tri-sodium ci...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13065-023-00915-4 |
| _version_ | 1797827730489737216 |
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| author | Magda Ali Akl Amira Mostafa Kamel Mahmoud Ahmed Abd El-Ghaffar |
| author_facet | Magda Ali Akl Amira Mostafa Kamel Mahmoud Ahmed Abd El-Ghaffar |
| author_sort | Magda Ali Akl |
| collection | DOAJ |
| description | Abstract In this study, Superparamagnetic magnetite nanoparticles (SPMNPs) are used in a new way as direct nanocarrier for Doxorubicin hydrochloride (DOX) via the functionalization of their surface with tri-sodium citrate through ligand exchange to conjugate DOX with imine bond to form tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (DOX/Cit-MNPs). The DOX/Cit-MNPs were coated with chitosan to form chitosan coated citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) to offer biodegradability and pH-sensitive drug release features. The Fourier transform infrared spectroscopy (FTIR) analysis confirmed functionalization of SPMNPs, DOX-conjugation, and chitosan coating. The trans electron microscopy (TEM) show spherical nanostructures with average size 40 nm for coated nanocarriers. The saturation magnetization value of carrier was 59 emu/g.The in-vitro release of DOX from the chitosan coated tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) was studied to be 75% at pH 5.5 and 28.6% at pH 7.4 which proves the pH sensitivity of encapsulated Cs/DOX/Cit-MNPs. The effect of Cs/DOX/Cit-MNPs toward Human Breast Cancer Cell lines (MCF7) was studied and found to be 76% without magnet and 98% with external magnet after 72 h. With increasing DOX concentration and treatment time, the cell inhibition (IR%) of DOX solution and Cs/DOX-Cit-MNPs suspension to all cells is increased. Cs/DOX/Cit-MNPs showed sustained release and good inhibition to cancer cells and offer a protective mode for normal cells (WISH) compared to the free DOX. Graphical Abstract |
| first_indexed | 2024-04-09T12:53:03Z |
| format | Article |
| id | doaj.art-b6f471f533704ddcbe8d817743abbe0b |
| institution | Directory Open Access Journal |
| issn | 2661-801X |
| language | English |
| last_indexed | 2024-04-09T12:53:03Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Chemistry |
| spelling | doaj.art-b6f471f533704ddcbe8d817743abbe0b2023-05-14T11:07:15ZengBMCBMC Chemistry2661-801X2023-02-0117111810.1186/s13065-023-00915-4Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinomaMagda Ali Akl0Amira Mostafa Kamel1Mahmoud Ahmed Abd El-Ghaffar2Chemistry Department, Faculty of Science, Mansoura UniversityPolymers and Pigments Department, National Research CentrePolymers and Pigments Department, National Research CentreAbstract In this study, Superparamagnetic magnetite nanoparticles (SPMNPs) are used in a new way as direct nanocarrier for Doxorubicin hydrochloride (DOX) via the functionalization of their surface with tri-sodium citrate through ligand exchange to conjugate DOX with imine bond to form tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (DOX/Cit-MNPs). The DOX/Cit-MNPs were coated with chitosan to form chitosan coated citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) to offer biodegradability and pH-sensitive drug release features. The Fourier transform infrared spectroscopy (FTIR) analysis confirmed functionalization of SPMNPs, DOX-conjugation, and chitosan coating. The trans electron microscopy (TEM) show spherical nanostructures with average size 40 nm for coated nanocarriers. The saturation magnetization value of carrier was 59 emu/g.The in-vitro release of DOX from the chitosan coated tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) was studied to be 75% at pH 5.5 and 28.6% at pH 7.4 which proves the pH sensitivity of encapsulated Cs/DOX/Cit-MNPs. The effect of Cs/DOX/Cit-MNPs toward Human Breast Cancer Cell lines (MCF7) was studied and found to be 76% without magnet and 98% with external magnet after 72 h. With increasing DOX concentration and treatment time, the cell inhibition (IR%) of DOX solution and Cs/DOX-Cit-MNPs suspension to all cells is increased. Cs/DOX/Cit-MNPs showed sustained release and good inhibition to cancer cells and offer a protective mode for normal cells (WISH) compared to the free DOX. Graphical Abstracthttps://doi.org/10.1186/s13065-023-00915-4Superparamagnetic magnetite nanoparticlesDoxorubicin hydrochlorideBinary targeting drug systemBreast carcinoma |
| spellingShingle | Magda Ali Akl Amira Mostafa Kamel Mahmoud Ahmed Abd El-Ghaffar Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma BMC Chemistry Superparamagnetic magnetite nanoparticles Doxorubicin hydrochloride Binary targeting drug system Breast carcinoma |
| title | Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma |
| title_full | Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma |
| title_fullStr | Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma |
| title_full_unstemmed | Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma |
| title_short | Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma |
| title_sort | biodegradable functionalized magnetite nanoparticles as binary targeting carrier for breast carcinoma |
| topic | Superparamagnetic magnetite nanoparticles Doxorubicin hydrochloride Binary targeting drug system Breast carcinoma |
| url | https://doi.org/10.1186/s13065-023-00915-4 |
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