miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ)
ABSTRACT: Poultry is susceptible to fatty liver which lead to decrease egg production and increase mortality. But the potential molecular mechanisms remain largely unclear. In the current study, in combination with transcriptome sequencing and miRNA sequencing data analysis from F1 generation of the...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122005144 |
| _version_ | 1797984224378093568 |
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| author | Yong-Hong Zhang Heng-Li Xie Yu-Wei Yang Jie Wen Ran-Ran Liu Gui-Ping Zhao Xiao-Dong Tan Zhen Liu Yi Zheng Jia-Bao Zhang |
| author_facet | Yong-Hong Zhang Heng-Li Xie Yu-Wei Yang Jie Wen Ran-Ran Liu Gui-Ping Zhao Xiao-Dong Tan Zhen Liu Yi Zheng Jia-Bao Zhang |
| author_sort | Yong-Hong Zhang |
| collection | DOAJ |
| description | ABSTRACT: Poultry is susceptible to fatty liver which lead to decrease egg production and increase mortality. But the potential molecular mechanisms remain largely unclear. In the current study, in combination with transcriptome sequencing and miRNA sequencing data analysis from F1 generation of the normal liver and fatty liver tissues, the differentially expressed miR-375 and its target gene RBPJ were screened and verified. The expression levels of miR-375 and RBPJ gene in the liver between control and fatty liver groups of F0-F3 generation for Jingxing-Huang (JXH) chicken are different significantly (P < 0.05 or P < 0.01). And downregulated RBPJ expression can promote TG content and lipid droplets in primary hepatocytes cultured in vitro (P < 0.01). Cell proliferation-related genes, including PMP22, IGF-1, IGF-2, and IGFBP-5, increased or decreased significantly after overexpression or knock-down RBPJ (P < 0.05 or P < 0.01), respectively. This study uniquely revealed that miR-375 induced lipid synthesis and inhibited cell proliferation may partly due to regulation of RBPJ expression, thereby involving in fatty liver formation and inheritance in chicken. The results could be useful in identifying candidate genes and revealing the pathogenesis of fatty liver that may be used for disease-resistance selective breeding in chicken. |
| first_indexed | 2024-04-11T06:58:11Z |
| format | Article |
| id | doaj.art-b6fa801049784ace833213bc67f30b78 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-11T06:58:11Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-b6fa801049784ace833213bc67f30b782022-12-22T04:38:56ZengElsevierPoultry Science0032-57912023-01-011021102218miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ)Yong-Hong Zhang0Heng-Li Xie1Yu-Wei Yang2Jie Wen3Ran-Ran Liu4Gui-Ping Zhao5Xiao-Dong Tan6Zhen Liu7Yi Zheng8Jia-Bao Zhang9College of Animal Science, Jilin University, Changchun 130062, P. R. ChinaCollege of Animal Science, Jilin University, Changchun 130062, P. R. ChinaCollege of Animal Science, Jilin University, Changchun 130062, P. R. ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaCollege of Animal Science, Jilin University, Changchun 130062, P. R. China; Corresponding author:ABSTRACT: Poultry is susceptible to fatty liver which lead to decrease egg production and increase mortality. But the potential molecular mechanisms remain largely unclear. In the current study, in combination with transcriptome sequencing and miRNA sequencing data analysis from F1 generation of the normal liver and fatty liver tissues, the differentially expressed miR-375 and its target gene RBPJ were screened and verified. The expression levels of miR-375 and RBPJ gene in the liver between control and fatty liver groups of F0-F3 generation for Jingxing-Huang (JXH) chicken are different significantly (P < 0.05 or P < 0.01). And downregulated RBPJ expression can promote TG content and lipid droplets in primary hepatocytes cultured in vitro (P < 0.01). Cell proliferation-related genes, including PMP22, IGF-1, IGF-2, and IGFBP-5, increased or decreased significantly after overexpression or knock-down RBPJ (P < 0.05 or P < 0.01), respectively. This study uniquely revealed that miR-375 induced lipid synthesis and inhibited cell proliferation may partly due to regulation of RBPJ expression, thereby involving in fatty liver formation and inheritance in chicken. The results could be useful in identifying candidate genes and revealing the pathogenesis of fatty liver that may be used for disease-resistance selective breeding in chicken.http://www.sciencedirect.com/science/article/pii/S0032579122005144chickenfatty livermiR-375RBPJlipid metabolism |
| spellingShingle | Yong-Hong Zhang Heng-Li Xie Yu-Wei Yang Jie Wen Ran-Ran Liu Gui-Ping Zhao Xiao-Dong Tan Zhen Liu Yi Zheng Jia-Bao Zhang miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ) Poultry Science chicken fatty liver miR-375 RBPJ lipid metabolism |
| title | miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ) |
| title_full | miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ) |
| title_fullStr | miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ) |
| title_full_unstemmed | miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ) |
| title_short | miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ) |
| title_sort | mir 375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa j region rbpj |
| topic | chicken fatty liver miR-375 RBPJ lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122005144 |
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