Characterization and mapping of an informational suppressor in Aspergillus nidulans
The present work was undertaken to characterize a suppressor gene present in a mutant strain of A. nidulans obtained with NTG (N-Methyl-N'-Nitro-N-Nitrosoguanidine). Analyses of this mutant have shown that this suppressor, designated suO1, induces phenotypic co-reversion of several auxotrophic...
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| Format: | Article |
| Language: | English |
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BMC
2000-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100008 |
| _version_ | 1818494047109513216 |
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| author | MELISSA GRACITE DE MELO FRANZONI MARIALBA AVEZUM ALVES DE CASTRO-PRADO |
| author_facet | MELISSA GRACITE DE MELO FRANZONI MARIALBA AVEZUM ALVES DE CASTRO-PRADO |
| author_sort | MELISSA GRACITE DE MELO FRANZONI |
| collection | DOAJ |
| description | The present work was undertaken to characterize a suppressor gene present in a mutant strain of A. nidulans obtained with NTG (N-Methyl-N'-Nitro-N-Nitrosoguanidine). Analyses of this mutant have shown that this suppressor, designated suO1, induces phenotypic co-reversion of several auxotrophic mutations and makes the strain sensitive to aminoglycoside antibiotics and lower temperatures. suO1 has shown to be on linkage group VIII. The vegetative growth of the mutant strain is very unstable because the suppressor gene induces the production of prototrophic mitotic sectors. The strains bearing the suO1 gene produce cleistothecia containing a reduced number of viable ascospores during the sexual cycle. The segregation of the genetic markers has also been observed in the mutant strain self crossed. From the above results it may be concluded that suO1 is an informational suppressor |
| first_indexed | 2024-12-10T18:01:31Z |
| format | Article |
| id | doaj.art-b6fc19351723460dbb53b7b62d7ea942 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-10T18:01:31Z |
| publishDate | 2000-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-b6fc19351723460dbb53b7b62d7ea9422022-12-22T01:38:45ZengBMCBiological Research0716-97600717-62872000-01-013311119Characterization and mapping of an informational suppressor in Aspergillus nidulansMELISSA GRACITE DE MELO FRANZONIMARIALBA AVEZUM ALVES DE CASTRO-PRADOThe present work was undertaken to characterize a suppressor gene present in a mutant strain of A. nidulans obtained with NTG (N-Methyl-N'-Nitro-N-Nitrosoguanidine). Analyses of this mutant have shown that this suppressor, designated suO1, induces phenotypic co-reversion of several auxotrophic mutations and makes the strain sensitive to aminoglycoside antibiotics and lower temperatures. suO1 has shown to be on linkage group VIII. The vegetative growth of the mutant strain is very unstable because the suppressor gene induces the production of prototrophic mitotic sectors. The strains bearing the suO1 gene produce cleistothecia containing a reduced number of viable ascospores during the sexual cycle. The segregation of the genetic markers has also been observed in the mutant strain self crossed. From the above results it may be concluded that suO1 is an informational suppressorhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100008Aspergillus nidulansbarren cleistotheciainformational suppressorparomomycin |
| spellingShingle | MELISSA GRACITE DE MELO FRANZONI MARIALBA AVEZUM ALVES DE CASTRO-PRADO Characterization and mapping of an informational suppressor in Aspergillus nidulans Biological Research Aspergillus nidulans barren cleistothecia informational suppressor paromomycin |
| title | Characterization and mapping of an informational suppressor in Aspergillus nidulans |
| title_full | Characterization and mapping of an informational suppressor in Aspergillus nidulans |
| title_fullStr | Characterization and mapping of an informational suppressor in Aspergillus nidulans |
| title_full_unstemmed | Characterization and mapping of an informational suppressor in Aspergillus nidulans |
| title_short | Characterization and mapping of an informational suppressor in Aspergillus nidulans |
| title_sort | characterization and mapping of an informational suppressor in aspergillus nidulans |
| topic | Aspergillus nidulans barren cleistothecia informational suppressor paromomycin |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100008 |
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