Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere
Abstract Results from previous experiments indicated that the Gram-negative α-proteobacterium Serratia liquefaciens strain ATCC 27592 was capable of growth under low temperature (0 °C), low pressure (0.7 kPa), and anoxic, CO2-dominated atmosphere–conditions intended to simulate the near-subsurface e...
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Nature Portfolio
2018-10-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-018-33140-4 |
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| author | Patricia Fajardo-Cavazos Michael D. Morrison Kathleen M. Miller Andrew C. Schuerger Wayne L. Nicholson |
| author_facet | Patricia Fajardo-Cavazos Michael D. Morrison Kathleen M. Miller Andrew C. Schuerger Wayne L. Nicholson |
| author_sort | Patricia Fajardo-Cavazos |
| collection | DOAJ |
| description | Abstract Results from previous experiments indicated that the Gram-negative α-proteobacterium Serratia liquefaciens strain ATCC 27592 was capable of growth under low temperature (0 °C), low pressure (0.7 kPa), and anoxic, CO2-dominated atmosphere–conditions intended to simulate the near-subsurface environment of Mars. To probe the response of its transcriptome to this extreme environment, S. liquefaciens ATCC 27592 was cultivated under 4 different environmental simulations: 0 °C, 0.7 kPa, CO2 atmosphere (Condition A); 0 °C, ~101.3 kPa, CO2 atmosphere (Condition B); 0 °C, ~101.3 kPa, ambient N2/O2 atmosphere (Condition C); and 30 °C, ~101.3 kPa, N2/O2 atmosphere (Condition D; ambient laboratory conditions). RNA-seq was performed on ribosomal RNA-depleted total RNA isolated from triplicate cultures grown under Conditions A-D and the datasets generated were subjected to transcriptome analyses. The data from Conditions A, B, or C were compared to laboratory Condition D. Significantly differentially expressed transcripts were identified belonging to a number of KEGG pathway categories. Up-regulated genes under all Conditions A, B, and C included those encoding transporters (ABC and PTS transporters); genes involved in translation (ribosomes and their biogenesis, biosynthesis of both tRNAs and aminoacyl-tRNAs); DNA repair and recombination; and non-coding RNAs. Genes down-regulated under all Conditions A, B, and C included: transporters (mostly ABC transporters); flagellar and motility proteins; genes involved in phenylalanine metabolism; transcription factors; and two-component systems. The results are discussed in the context of Mars astrobiology and planetary protection. |
| first_indexed | 2024-12-20T20:45:09Z |
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| language | English |
| last_indexed | 2024-12-20T20:45:09Z |
| publishDate | 2018-10-01 |
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| series | Scientific Reports |
| spelling | doaj.art-b6fdb3d77da74d8ea5c3f677a668f8572022-12-21T19:27:03ZengNature PortfolioScientific Reports2045-23222018-10-018111010.1038/s41598-018-33140-4Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmospherePatricia Fajardo-Cavazos0Michael D. Morrison1Kathleen M. Miller2Andrew C. Schuerger3Wayne L. Nicholson4Department of Microbiology and Cell Science, University of FloridaDepartment of Microbiology and Cell Science, University of FloridaDepartment of Microbiology and Cell Science, University of FloridaDepartment of Plant Pathology, University of FloridaDepartment of Microbiology and Cell Science, University of FloridaAbstract Results from previous experiments indicated that the Gram-negative α-proteobacterium Serratia liquefaciens strain ATCC 27592 was capable of growth under low temperature (0 °C), low pressure (0.7 kPa), and anoxic, CO2-dominated atmosphere–conditions intended to simulate the near-subsurface environment of Mars. To probe the response of its transcriptome to this extreme environment, S. liquefaciens ATCC 27592 was cultivated under 4 different environmental simulations: 0 °C, 0.7 kPa, CO2 atmosphere (Condition A); 0 °C, ~101.3 kPa, CO2 atmosphere (Condition B); 0 °C, ~101.3 kPa, ambient N2/O2 atmosphere (Condition C); and 30 °C, ~101.3 kPa, N2/O2 atmosphere (Condition D; ambient laboratory conditions). RNA-seq was performed on ribosomal RNA-depleted total RNA isolated from triplicate cultures grown under Conditions A-D and the datasets generated were subjected to transcriptome analyses. The data from Conditions A, B, or C were compared to laboratory Condition D. Significantly differentially expressed transcripts were identified belonging to a number of KEGG pathway categories. Up-regulated genes under all Conditions A, B, and C included those encoding transporters (ABC and PTS transporters); genes involved in translation (ribosomes and their biogenesis, biosynthesis of both tRNAs and aminoacyl-tRNAs); DNA repair and recombination; and non-coding RNAs. Genes down-regulated under all Conditions A, B, and C included: transporters (mostly ABC transporters); flagellar and motility proteins; genes involved in phenylalanine metabolism; transcription factors; and two-component systems. The results are discussed in the context of Mars astrobiology and planetary protection.https://doi.org/10.1038/s41598-018-33140-4Simulated Mars ConditionsTranscriptomic ResponseKyoto Encyclopedia Of Genes And Genomes (KEGG)KEGG Pathway CategoriesPlanetary Protection |
| spellingShingle | Patricia Fajardo-Cavazos Michael D. Morrison Kathleen M. Miller Andrew C. Schuerger Wayne L. Nicholson Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere Scientific Reports Simulated Mars Conditions Transcriptomic Response Kyoto Encyclopedia Of Genes And Genomes (KEGG) KEGG Pathway Categories Planetary Protection |
| title | Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere |
| title_full | Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere |
| title_fullStr | Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere |
| title_full_unstemmed | Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere |
| title_short | Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere |
| title_sort | transcriptomic responses of serratia liquefaciens cells grown under simulated martian conditions of low temperature low pressure and co2 enriched anoxic atmosphere |
| topic | Simulated Mars Conditions Transcriptomic Response Kyoto Encyclopedia Of Genes And Genomes (KEGG) KEGG Pathway Categories Planetary Protection |
| url | https://doi.org/10.1038/s41598-018-33140-4 |
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