Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassay
A sensitive and specific double antibody radioimmunoassay for the major apolipoprotein (apo A-I) of human serum high density lipoprotein (HDL) was developed. Initial studies indicated that direct measurements of apo A-I concentration in whole untreated sera or isolated high density lipoprotein fract...
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| Format: | Article |
| Language: | English |
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Elsevier
1976-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520370139 |
| _version_ | 1818383816538980352 |
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| author | J B Karlin D J Juhn J I Starr A M Scanu A H Rubenstein |
| author_facet | J B Karlin D J Juhn J I Starr A M Scanu A H Rubenstein |
| author_sort | J B Karlin |
| collection | DOAJ |
| description | A sensitive and specific double antibody radioimmunoassay for the major apolipoprotein (apo A-I) of human serum high density lipoprotein (HDL) was developed. Initial studies indicated that direct measurements of apo A-I concentration in whole untreated sera or isolated high density lipoprotein fractions yielded variable results, which were lower than those obtained in the corresponding samples which had been subjected to delipidation. Subsequently, it was observed that heating diluted sera or HDL for 3 hr at 52 °C prior to assay resulted in maximal increases in apo A-I immunoreactivity to levels comparable to those found in the delipidated specimens. This simple procedure permitted multiple sera to be assayed efficiently with full recovery of apo A-I.Serum apo A-I in healthy normolipemic males was 130 ± 3 mg/dl (range 95–165), while the values in females were significantly higher 154 ± 6 mg/dl (range 107–199) (P < 0.005). The apo A-I levels correlated with the total serum cholesterol (males r = 0.46, P < 0.005; females r = 0.58, P < 0.001).Preliminary results with sera from patients with abetalipoproteinemia, hypercholeskrdemia and Tangier disease indicated that alterations in the low density lipoprotein concentration and changes in the physical chemical properties of the high density lipoproteins did not affect the optimal conditions for mnwuring serum apo A-I. The apo A-I concentrations were abnormally low in each of the above disorders. |
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| format | Article |
| id | doaj.art-b70390a8ead24de8ad0614399b3bd2b9 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:12:23Z |
| publishDate | 1976-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-b70390a8ead24de8ad0614399b3bd2b92022-12-21T23:19:15ZengElsevierJournal of Lipid Research0022-22751976-01-011713037Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassayJ B Karlin0D J Juhn1J I Starr2A M Scanu3A H Rubenstein4Departments of Medicine and Biochemistry, The University of Chicago Pritzker School of Medicine, Chicsgo, Illinois 80637Departments of Medicine and Biochemistry, The University of Chicago Pritzker School of Medicine, Chicsgo, Illinois 80637Departments of Medicine and Biochemistry, The University of Chicago Pritzker School of Medicine, Chicsgo, Illinois 80637Departments of Medicine and Biochemistry, The University of Chicago Pritzker School of Medicine, Chicsgo, Illinois 80637Departments of Medicine and Biochemistry, The University of Chicago Pritzker School of Medicine, Chicsgo, Illinois 80637; Reprint request should be addressed to Dr. Arthur H. Rubenstein, Department of Medicine, The University of Chicago, Box 435, 950 East 59th Street, Chicago, Illinois 60637A sensitive and specific double antibody radioimmunoassay for the major apolipoprotein (apo A-I) of human serum high density lipoprotein (HDL) was developed. Initial studies indicated that direct measurements of apo A-I concentration in whole untreated sera or isolated high density lipoprotein fractions yielded variable results, which were lower than those obtained in the corresponding samples which had been subjected to delipidation. Subsequently, it was observed that heating diluted sera or HDL for 3 hr at 52 °C prior to assay resulted in maximal increases in apo A-I immunoreactivity to levels comparable to those found in the delipidated specimens. This simple procedure permitted multiple sera to be assayed efficiently with full recovery of apo A-I.Serum apo A-I in healthy normolipemic males was 130 ± 3 mg/dl (range 95–165), while the values in females were significantly higher 154 ± 6 mg/dl (range 107–199) (P < 0.005). The apo A-I levels correlated with the total serum cholesterol (males r = 0.46, P < 0.005; females r = 0.58, P < 0.001).Preliminary results with sera from patients with abetalipoproteinemia, hypercholeskrdemia and Tangier disease indicated that alterations in the low density lipoprotein concentration and changes in the physical chemical properties of the high density lipoproteins did not affect the optimal conditions for mnwuring serum apo A-I. The apo A-I concentrations were abnormally low in each of the above disorders.http://www.sciencedirect.com/science/article/pii/S0022227520370139High density lipoprotein radioimmunoassayapolipoprotein A-IabetalipoproteinemiahypercholesterolemiaTangier disease |
| spellingShingle | J B Karlin D J Juhn J I Starr A M Scanu A H Rubenstein Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassay Journal of Lipid Research High density lipoprotein radioimmunoassay apolipoprotein A-I abetalipoproteinemia hypercholesterolemia Tangier disease |
| title | Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassay |
| title_full | Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassay |
| title_fullStr | Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassay |
| title_full_unstemmed | Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassay |
| title_short | Measurement of human high density lipoprotein apolipoprotein A-I in serum by radioimmunoassay |
| title_sort | measurement of human high density lipoprotein apolipoprotein a i in serum by radioimmunoassay |
| topic | High density lipoprotein radioimmunoassay apolipoprotein A-I abetalipoproteinemia hypercholesterolemia Tangier disease |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520370139 |
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