Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium
ObjectiveTo investigate the protective effect of quercetin on cadmium-mediated lipid metabolism disorder in livers of mice.MethodsC57BL/6J mice were divided into four groups: a control group, a quercetin-treated group (quercetin by intragastrical administration), a cadmium-treated group (cadmium chl...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Laboratory Animal and Comparative Medicine
2021-08-01
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| Series: | Shiyan dongwu yu bijiao yixue |
| Subjects: | |
| Online Access: | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.007 |
| _version_ | 1798016501629845504 |
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| author | LI Zifa ZHANG Hao REN Meng XU Kaiyong HU Minghui ZHOU Miaomiao WANG Kezhou |
| author_facet | LI Zifa ZHANG Hao REN Meng XU Kaiyong HU Minghui ZHOU Miaomiao WANG Kezhou |
| author_sort | LI Zifa |
| collection | DOAJ |
| description | ObjectiveTo investigate the protective effect of quercetin on cadmium-mediated lipid metabolism disorder in livers of mice.MethodsC57BL/6J mice were divided into four groups: a control group, a quercetin-treated group (quercetin by intragastrical administration), a cadmium-treated group (cadmium chloride by intragastrical administration), and a cadmium plus quercetin co-treated group (cadmium chloride plus quercetin by intragastrical administration). After the administration, mice weight was measured; the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, and the contents of total cholesterol (TC) and triglyceride (TG) in both serum and liver were determined; the pathological changes in liver tissues and liver droplets were observed by HE and Oil Red O staining; the expression levels of proteins related to lipogenesis, fatty acid synthase (FAS), and related to lipolysis, lipoprotein lipase (LPL), in liver were detected by Western blotting.ResultsCompared with the control group, the mice treated with cadmium showed that the weight was significantly decreased (P<0.01), the ALT and AST activities were remarkedly increased in serum (P<0.01), the pathological changes of liver injury were seen obviously, the TC and TG contents were remarkedly elevated in both serum and liver (P<0.01), lipid droplets were greatly accumulated (P<0.01), the FAS expression in liver was up-regulated (P<0.01), while the LPL expression was down-regulated (P<0.01). Quercetin intervention significantly alleviated the abnormal changes of the above indicators.ConclusionQuercetin can protect mice from cadmium-induced liver injury by regulating lipid metabolism disorder in liver. |
| first_indexed | 2024-04-11T15:51:34Z |
| format | Article |
| id | doaj.art-b7047b1d2edd492ba4bee2f753ded3b8 |
| institution | Directory Open Access Journal |
| issn | 1674-5817 |
| language | zho |
| last_indexed | 2024-04-11T15:51:34Z |
| publishDate | 2021-08-01 |
| publisher | Editorial Office of Laboratory Animal and Comparative Medicine |
| record_format | Article |
| series | Shiyan dongwu yu bijiao yixue |
| spelling | doaj.art-b7047b1d2edd492ba4bee2f753ded3b82022-12-22T04:15:17ZzhoEditorial Office of Laboratory Animal and Comparative MedicineShiyan dongwu yu bijiao yixue1674-58172021-08-0141430531210.12300/j.issn.1674-5817.2021.0071674-5817（2021）04-0305-08Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by CadmiumLI Zifa0ZHANG Hao1REN Meng2XU Kaiyong3HU Minghui4ZHOU Miaomiao5WANG Kezhou6Laboratory Animal Center, Shandong University of Traditional Chinese Medicine, Jinan 250355 ChinaLaboratory Animal Center, Shandong University of Traditional Chinese Medicine, Jinan 250355 ChinaLaboratory Animal Center, Shandong University of Traditional Chinese Medicine, Jinan 250355 ChinaLaboratory Animal Center, Shandong University of Traditional Chinese Medicine, Jinan 250355 ChinaLaboratory Animal Center, Shandong University of Traditional Chinese Medicine, Jinan 250355 ChinaLaboratory Animal Center, Shandong University of Traditional Chinese Medicine, Jinan 250355 ChinaCollege of Laboratory Animal (Shandong Laboratory Animal Center), Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan 250002, ChinaObjectiveTo investigate the protective effect of quercetin on cadmium-mediated lipid metabolism disorder in livers of mice.MethodsC57BL/6J mice were divided into four groups: a control group, a quercetin-treated group (quercetin by intragastrical administration), a cadmium-treated group (cadmium chloride by intragastrical administration), and a cadmium plus quercetin co-treated group (cadmium chloride plus quercetin by intragastrical administration). After the administration, mice weight was measured; the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, and the contents of total cholesterol (TC) and triglyceride (TG) in both serum and liver were determined; the pathological changes in liver tissues and liver droplets were observed by HE and Oil Red O staining; the expression levels of proteins related to lipogenesis, fatty acid synthase (FAS), and related to lipolysis, lipoprotein lipase (LPL), in liver were detected by Western blotting.ResultsCompared with the control group, the mice treated with cadmium showed that the weight was significantly decreased (P<0.01), the ALT and AST activities were remarkedly increased in serum (P<0.01), the pathological changes of liver injury were seen obviously, the TC and TG contents were remarkedly elevated in both serum and liver (P<0.01), lipid droplets were greatly accumulated (P<0.01), the FAS expression in liver was up-regulated (P<0.01), while the LPL expression was down-regulated (P<0.01). Quercetin intervention significantly alleviated the abnormal changes of the above indicators.ConclusionQuercetin can protect mice from cadmium-induced liver injury by regulating lipid metabolism disorder in liver.https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.007quercetincadmiumliver damagelipid metabolismc57bl/6j mice |
| spellingShingle | LI Zifa ZHANG Hao REN Meng XU Kaiyong HU Minghui ZHOU Miaomiao WANG Kezhou Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium Shiyan dongwu yu bijiao yixue quercetin cadmium liver damage lipid metabolism c57bl/6j mice |
| title | Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium |
| title_full | Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium |
| title_fullStr | Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium |
| title_full_unstemmed | Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium |
| title_short | Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium |
| title_sort | protective effect of quercetin on lipid metabolism disorder in mice livers caused by cadmium |
| topic | quercetin cadmium liver damage lipid metabolism c57bl/6j mice |
| url | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.007 |
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