Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsy
Temporal lobe epilepsy (TLE) is the most prevalent form of epilepsy, through the neuronal mechanisms of this syndrome remain elusive. In addition to the temporal lobe structures, it was found that the basal forebrain cholinergic cells are also involved in epileptogenesis. However, little is known ab...
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AIMS Press
2023-11-01
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| Series: | AIMS Neuroscience |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/Neuroscience.2023023?viewType=HTML |
| _version_ | 1797366818875113472 |
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| author | Ruben Carvalho Alisa N. Lukoyanova João Casalta-Lopes Nikolay V. Lukoyanov Joana Isabel Soares |
| author_facet | Ruben Carvalho Alisa N. Lukoyanova João Casalta-Lopes Nikolay V. Lukoyanov Joana Isabel Soares |
| author_sort | Ruben Carvalho |
| collection | DOAJ |
| description | Temporal lobe epilepsy (TLE) is the most prevalent form of epilepsy, through the neuronal mechanisms of this syndrome remain elusive. In addition to the temporal lobe structures, it was found that the basal forebrain cholinergic cells are also involved in epileptogenesis. However, little is known about the involvement of the basal forebrain GABAergic neurons in epilepsy; despite this, they largely project to the temporal lobe and are crucial for the regulation of the hippocampal circuitry. In this study, we assessed epilepsy-induced changes in parvalbumin (PARV) immunoreactive neurons of the medial septum (MS) and of the magnocellular preoptic nucleus (MCPO) using the kainic acid (KA) model in rats. In addition, we estimated the respective changes in the cholinergic varicosities in the MS, where we observed a significant reduction in the PARV cell number (12849 ± 2715 vs. 9372 ± 1336, <i>p</i> = .029) and density (16.2 ± 2.62 vs. 10.5 ± 1.00 per .001 mm<sup>3</sup>, <i>p</i> =.001), and an increase in the density of cholinergic varicosities (47.9 ± 11.1 vs. 69.4 ± 17.8 per 30,000 µm<sup>2</sup>, <i>p</i> =.036) in KA-treated animals. In the MCPO, these animals showed a significant increase in somatic volume (827.9 ± 235.2 µm<sup>3</sup> vs. 469.9 ± 79.6 µm<sup>3</sup>, <i>p</i> = .012) and total cell number (2268.6 ± 707.1 vs. 1362.4 ± 262.0, <i>p</i> =.028). These results show that the basal forebrain GABAergic cell populations undergo numerical and morphological changes in epileptic animals, which may contribute to an increased vulnerability of brain circuits to epilepsy and epilepsy-related functional impairments. |
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| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-08T17:09:08Z |
| publishDate | 2023-11-01 |
| publisher | AIMS Press |
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| series | AIMS Neuroscience |
| spelling | doaj.art-b707a68269a642888e2879acde8c060e2024-01-04T01:14:02ZengAIMS PressAIMS Neuroscience2373-79722023-11-0110430031410.3934/Neuroscience.2023023Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsyRuben Carvalho0Alisa N. Lukoyanova1João Casalta-Lopes 2Nikolay V. Lukoyanov3Joana Isabel Soares41. Master in Neurobiology, Faculty of Medicine, University of Porto, Porto, Portugal 2. Neuronal Networks Group, Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, Porto, Portugal 3. Department of Biomedicine, Faculty of Medicine, University of Porto, Porto, Portugal2. Neuronal Networks Group, Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, Porto, Portugal4. Department of Basic Sciences, Polytechnic Institute of Coimbra, Coimbra Health School, Coimbra, Portugal 5. Life and Health Sciences Research Institute / School of Medicine - University of Minho, Braga, Portugal 6. Department of Radiotherapy, University Hospital Center of São João, Porto, Portugal2. Neuronal Networks Group, Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, Porto, Portugal 3. Department of Biomedicine, Faculty of Medicine, University of Porto, Porto, Port2. Neuronal Networks Group, Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, Porto, Portugal 3. Department of Biomedicine, Faculty of Medicine, University of Porto, Porto, Portugal 4. Department of Basic Sciences, Polytechnic Institute of Coimbra, Coimbra Health School, Coimbra, PortugalTemporal lobe epilepsy (TLE) is the most prevalent form of epilepsy, through the neuronal mechanisms of this syndrome remain elusive. In addition to the temporal lobe structures, it was found that the basal forebrain cholinergic cells are also involved in epileptogenesis. However, little is known about the involvement of the basal forebrain GABAergic neurons in epilepsy; despite this, they largely project to the temporal lobe and are crucial for the regulation of the hippocampal circuitry. In this study, we assessed epilepsy-induced changes in parvalbumin (PARV) immunoreactive neurons of the medial septum (MS) and of the magnocellular preoptic nucleus (MCPO) using the kainic acid (KA) model in rats. In addition, we estimated the respective changes in the cholinergic varicosities in the MS, where we observed a significant reduction in the PARV cell number (12849 ± 2715 vs. 9372 ± 1336, <i>p</i> = .029) and density (16.2 ± 2.62 vs. 10.5 ± 1.00 per .001 mm<sup>3</sup>, <i>p</i> =.001), and an increase in the density of cholinergic varicosities (47.9 ± 11.1 vs. 69.4 ± 17.8 per 30,000 µm<sup>2</sup>, <i>p</i> =.036) in KA-treated animals. In the MCPO, these animals showed a significant increase in somatic volume (827.9 ± 235.2 µm<sup>3</sup> vs. 469.9 ± 79.6 µm<sup>3</sup>, <i>p</i> = .012) and total cell number (2268.6 ± 707.1 vs. 1362.4 ± 262.0, <i>p</i> =.028). These results show that the basal forebrain GABAergic cell populations undergo numerical and morphological changes in epileptic animals, which may contribute to an increased vulnerability of brain circuits to epilepsy and epilepsy-related functional impairments.https://www.aimspress.com/article/doi/10.3934/Neuroscience.2023023?viewType=HTMLtemporal lobe epilepsykainic-acid modelmagnocellular preoptic nucleusmedial septumparvalbuminneurophysiology |
| spellingShingle | Ruben Carvalho Alisa N. Lukoyanova João Casalta-Lopes Nikolay V. Lukoyanov Joana Isabel Soares Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsy AIMS Neuroscience temporal lobe epilepsy kainic-acid model magnocellular preoptic nucleus medial septum parvalbumin neurophysiology |
| title | Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsy |
| title_full | Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsy |
| title_fullStr | Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsy |
| title_full_unstemmed | Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsy |
| title_short | Plastic rearrangement of basal forebrain parvalbumin-immunoreactive neurons in the kainite model of epilepsy |
| title_sort | plastic rearrangement of basal forebrain parvalbumin immunoreactive neurons in the kainite model of epilepsy |
| topic | temporal lobe epilepsy kainic-acid model magnocellular preoptic nucleus medial septum parvalbumin neurophysiology |
| url | https://www.aimspress.com/article/doi/10.3934/Neuroscience.2023023?viewType=HTML |
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