Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules
Transdermal drug delivery represents an appealing alternative to conventional drug administration systems. In fact, due to their high patient compliance, the development of dissolvable and biodegradable polymer microneedles has recently attracted great attention. Although stamp-based procedures guar...
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Format: | Article |
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Frontiers Media S.A.
2019-11-01
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Series: | Frontiers in Bioengineering and Biotechnology |
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Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00296/full |
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author | Mario Battisti Raffaele Vecchione Costantino Casale Fabrizio A. Pennacchio Vincenzo Lettera Rezvan Jamaledin Martina Profeta Concetta Di Natale Giorgia Imparato Francesco Urciuolo Francesco Urciuolo Paolo Antonio Netti Paolo Antonio Netti Paolo Antonio Netti |
author_facet | Mario Battisti Raffaele Vecchione Costantino Casale Fabrizio A. Pennacchio Vincenzo Lettera Rezvan Jamaledin Martina Profeta Concetta Di Natale Giorgia Imparato Francesco Urciuolo Francesco Urciuolo Paolo Antonio Netti Paolo Antonio Netti Paolo Antonio Netti |
author_sort | Mario Battisti |
collection | DOAJ |
description | Transdermal drug delivery represents an appealing alternative to conventional drug administration systems. In fact, due to their high patient compliance, the development of dissolvable and biodegradable polymer microneedles has recently attracted great attention. Although stamp-based procedures guarantee high tip resolution and reproducibility, they have long processing times, low levels of system engineering, are a source of possible contaminants, and thermo-sensitive drugs cannot be used in conjunction with them. In this work, a novel stamp-based microneedle fabrication method is proposed. It provides a rapid room-temperature production of multi-compartmental biodegradable polymeric microneedles for controlled intradermal drug release. Solvent casting was carried out for only a few minutes and produced a short dissolvable tip made of polyvinylpyrrolidone (PVP). The rest of the stamp was then filled with degradable poly(lactide-co-glycolide) (PLGA) microparticles (μPs) quickly compacted with a vapor-assisted plasticization. The outcome was an array of microneedles with tunable release. The ability of the resulting microneedles to indent was assessed using pig cadaver skin. Controlled intradermal delivery was demonstrated by loading both the tip and the body of the microneedles with model therapeutics; POXA1b laccase from Pleurotus ostreatus is a commercial enzyme used for the whitening of skin spots. The action and indentation of the enzyme-loaded microneedle action were assessed in an in vitro skin model and this highlighted their ability to control the kinetic release of the encapsulated compound. |
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issn | 2296-4185 |
language | English |
last_indexed | 2024-12-12T04:06:23Z |
publishDate | 2019-11-01 |
publisher | Frontiers Media S.A. |
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series | Frontiers in Bioengineering and Biotechnology |
spelling | doaj.art-b70c0937842443f9a9470861b7caf3772022-12-22T00:38:45ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-11-01710.3389/fbioe.2019.00296482506Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile MoleculesMario Battisti0Raffaele Vecchione1Costantino Casale2Fabrizio A. Pennacchio3Vincenzo Lettera4Rezvan Jamaledin5Martina Profeta6Concetta Di Natale7Giorgia Imparato8Francesco Urciuolo9Francesco Urciuolo10Paolo Antonio Netti11Paolo Antonio Netti12Paolo Antonio Netti13Center for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyCenter for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyInterdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Naples, ItalyCenter for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyBiopox Srl, Naples, ItalyCenter for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyCenter for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyCenter for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyCenter for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyInterdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Naples, ItalyDepartment of Chemical Materials and Industrial Production (DICMAPI), University of Naples Federico II, Naples, ItalyCenter for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, ItalyInterdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Naples, ItalyDepartment of Chemical Materials and Industrial Production (DICMAPI), University of Naples Federico II, Naples, ItalyTransdermal drug delivery represents an appealing alternative to conventional drug administration systems. In fact, due to their high patient compliance, the development of dissolvable and biodegradable polymer microneedles has recently attracted great attention. Although stamp-based procedures guarantee high tip resolution and reproducibility, they have long processing times, low levels of system engineering, are a source of possible contaminants, and thermo-sensitive drugs cannot be used in conjunction with them. In this work, a novel stamp-based microneedle fabrication method is proposed. It provides a rapid room-temperature production of multi-compartmental biodegradable polymeric microneedles for controlled intradermal drug release. Solvent casting was carried out for only a few minutes and produced a short dissolvable tip made of polyvinylpyrrolidone (PVP). The rest of the stamp was then filled with degradable poly(lactide-co-glycolide) (PLGA) microparticles (μPs) quickly compacted with a vapor-assisted plasticization. The outcome was an array of microneedles with tunable release. The ability of the resulting microneedles to indent was assessed using pig cadaver skin. Controlled intradermal delivery was demonstrated by loading both the tip and the body of the microneedles with model therapeutics; POXA1b laccase from Pleurotus ostreatus is a commercial enzyme used for the whitening of skin spots. The action and indentation of the enzyme-loaded microneedle action were assessed in an in vitro skin model and this highlighted their ability to control the kinetic release of the encapsulated compound.https://www.frontiersin.org/article/10.3389/fbioe.2019.00296/fullpolymer microneedlesmulti-compartmentalenzymecontrolled releaseskin model |
spellingShingle | Mario Battisti Raffaele Vecchione Costantino Casale Fabrizio A. Pennacchio Vincenzo Lettera Rezvan Jamaledin Martina Profeta Concetta Di Natale Giorgia Imparato Francesco Urciuolo Francesco Urciuolo Paolo Antonio Netti Paolo Antonio Netti Paolo Antonio Netti Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules Frontiers in Bioengineering and Biotechnology polymer microneedles multi-compartmental enzyme controlled release skin model |
title | Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules |
title_full | Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules |
title_fullStr | Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules |
title_full_unstemmed | Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules |
title_short | Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules |
title_sort | non invasive production of multi compartmental biodegradable polymer microneedles for controlled intradermal drug release of labile molecules |
topic | polymer microneedles multi-compartmental enzyme controlled release skin model |
url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00296/full |
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