Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse model
ABSTRACT The flavonoid kaempferol has attracted research attention as a potential adjuvant during chemotherapy. This study aimed to evaluate the protective effects of kaempferol against ovarian damage in cisplatin-treated mice. Two groups of mice received saline solution (intraperitoneal injection [...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Minas Gerais
2022-10-01
|
| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000500778&tlng=en |
| _version_ | 1811251553049247744 |
|---|---|
| author | L.M.R. Barbosa R.S. Barberino B.B. Gouveia V.G. Menezes R.C. Palheta Junior M.H.T. Matos |
| author_facet | L.M.R. Barbosa R.S. Barberino B.B. Gouveia V.G. Menezes R.C. Palheta Junior M.H.T. Matos |
| author_sort | L.M.R. Barbosa |
| collection | DOAJ |
| description | ABSTRACT The flavonoid kaempferol has attracted research attention as a potential adjuvant during chemotherapy. This study aimed to evaluate the protective effects of kaempferol against ovarian damage in cisplatin-treated mice. Two groups of mice received saline solution (intraperitoneal injection [i.p.]; control) or a single dose of cisplatin (5 mg/kg body weight, i.p.). Moreover, two other mice groups were pretreated with kaempferol (1 or 10 mg/kg body weight, i.p.) 30 min before of the cisplatin administration. Thereafter, their ovaries were harvested and subjected to histological (follicular morphology and activation) and fluorescence (reactive oxygen species [ROS] production, glutathione [GSH] concentration, and mitochondrial activity) analyses. Compared with cisplatin treatment alone, pretreatment with 1 mg/kg kaempferol maintained normal follicular morphology, reduced ROS production and mitochondrial damage, and enhanced GSH concentration. However, pretreatment with 10 mg/kg kaempferol did not prevent cisplatin-induced damage. The rate of primordial follicle activation was greater in mice pretreated with 1 mg/kg kaempferol than in the other treatment groups. In conclusion, pretreatment with 1 mg/kg kaempferol prevents cisplatin-induced ovarian damage and stimulates primordial follicle activation in mice. |
| first_indexed | 2024-04-12T16:22:12Z |
| format | Article |
| id | doaj.art-b70cf883e9d443dd87c70e9f0e6a6a33 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-04-12T16:22:12Z |
| publishDate | 2022-10-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-b70cf883e9d443dd87c70e9f0e6a6a332022-12-22T03:25:32ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622022-10-0174577878410.1590/1678-4162-12608Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse modelL.M.R. Barbosahttps://orcid.org/0000-0002-5870-6466R.S. Barberinohttps://orcid.org/0000-0001-5612-8909B.B. Gouveiahttps://orcid.org/0000-0001-8911-4298V.G. Menezeshttps://orcid.org/0000-0001-8330-3450R.C. Palheta Juniorhttps://orcid.org/0000-0003-1020-6291M.H.T. Matoshttps://orcid.org/0000-0001-6215-5292ABSTRACT The flavonoid kaempferol has attracted research attention as a potential adjuvant during chemotherapy. This study aimed to evaluate the protective effects of kaempferol against ovarian damage in cisplatin-treated mice. Two groups of mice received saline solution (intraperitoneal injection [i.p.]; control) or a single dose of cisplatin (5 mg/kg body weight, i.p.). Moreover, two other mice groups were pretreated with kaempferol (1 or 10 mg/kg body weight, i.p.) 30 min before of the cisplatin administration. Thereafter, their ovaries were harvested and subjected to histological (follicular morphology and activation) and fluorescence (reactive oxygen species [ROS] production, glutathione [GSH] concentration, and mitochondrial activity) analyses. Compared with cisplatin treatment alone, pretreatment with 1 mg/kg kaempferol maintained normal follicular morphology, reduced ROS production and mitochondrial damage, and enhanced GSH concentration. However, pretreatment with 10 mg/kg kaempferol did not prevent cisplatin-induced damage. The rate of primordial follicle activation was greater in mice pretreated with 1 mg/kg kaempferol than in the other treatment groups. In conclusion, pretreatment with 1 mg/kg kaempferol prevents cisplatin-induced ovarian damage and stimulates primordial follicle activation in mice.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000500778&tlng=enantioxidantchemotherapyovaryoxidative stressprimordial follicle |
| spellingShingle | L.M.R. Barbosa R.S. Barberino B.B. Gouveia V.G. Menezes R.C. Palheta Junior M.H.T. Matos Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse model Arquivo Brasileiro de Medicina Veterinária e Zootecnia antioxidant chemotherapy ovary oxidative stress primordial follicle |
| title | Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse model |
| title_full | Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse model |
| title_fullStr | Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse model |
| title_full_unstemmed | Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse model |
| title_short | Protective effect of kaempferol against cisplatin-induced acute ovarian damage in a mouse model |
| title_sort | protective effect of kaempferol against cisplatin induced acute ovarian damage in a mouse model |
| topic | antioxidant chemotherapy ovary oxidative stress primordial follicle |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000500778&tlng=en |
| work_keys_str_mv | AT lmrbarbosa protectiveeffectofkaempferolagainstcisplatininducedacuteovariandamageinamousemodel AT rsbarberino protectiveeffectofkaempferolagainstcisplatininducedacuteovariandamageinamousemodel AT bbgouveia protectiveeffectofkaempferolagainstcisplatininducedacuteovariandamageinamousemodel AT vgmenezes protectiveeffectofkaempferolagainstcisplatininducedacuteovariandamageinamousemodel AT rcpalhetajunior protectiveeffectofkaempferolagainstcisplatininducedacuteovariandamageinamousemodel AT mhtmatos protectiveeffectofkaempferolagainstcisplatininducedacuteovariandamageinamousemodel |