Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity.
OBJECTIVE:To investigate gut dysbiosis in patients with Sjögren's syndrome (SS) or dry eye syndrome (DES) compared to normal subjects and to evaluate the association of dysbiosis with dry eye severity. METHODS:10 subjects with SS, 14 subjects with DES and 12 controls were enrolled. Corneal stai...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0229029 |
| _version_ | 1828958486341353472 |
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| author | Jayoon Moon Se Hyun Choi Chang Ho Yoon Mee Kum Kim |
| author_facet | Jayoon Moon Se Hyun Choi Chang Ho Yoon Mee Kum Kim |
| author_sort | Jayoon Moon |
| collection | DOAJ |
| description | OBJECTIVE:To investigate gut dysbiosis in patients with Sjögren's syndrome (SS) or dry eye syndrome (DES) compared to normal subjects and to evaluate the association of dysbiosis with dry eye severity. METHODS:10 subjects with SS, 14 subjects with DES and 12 controls were enrolled. Corneal staining, tear break up time (TBUT) and tear secretion were evaluated. Bacterial genomic 16s rRNA from stool samples were analyzed. Main outcomes were microbiome compositional differences among groups and their correlation to dry eye signs. RESULTS:Gut microbiome analysis revealed significant compositional differences in SS compared to controls and DES. In phylum, Bacteriodetes increased, while Firmicutes/Bacteroidetes ratio and Actinobacteria decreased (p<0.05). In genus, Bifidobacterium was reduced (vs controls; p = 0.025, vs DES; p = 0.026). Beta diversity of SS also showed significant distances from controls and DES (p = 0.007 and 0.019, respectively). SS showed decreased genus of Blautia (p = 0.041), Dorea (p = 0.025) and Agathobacter (p = 0.035) compared to controls and increased genus of Prevotella (p = 0.026), Odoribacter (p = 0.028) and Alistipes (p = 0.46) compared to DES. On the other hand, DES only had increased genus Veillonella (p = 0.045) and reduced Subdoligranulum (p = 0.035) compared to controls. Bacteroidetes, Actinobacteria and Bifidobacterium were significantly related with dry eye signs (p<0.05). After adjustment of age, gender and group classification, multivariate linear regression analysis revealed tear secretion was strongly affected by Prevotella (p = 0.025). With additional adjustment of hydroxychloroquine use, TBUT was markedly affected by Prevotella (p = 0.037) and Actinobacteria (p = 0.001). CONCLUSIONS:Sjögren's syndrome showed significant gut dysbiosis compared to controls and environmental dry eye syndrome, while dry eye patients showed compositional changes of gut microbiome somewhere in between Sjögren's syndrome and controls. Dysbiosis of the gut microbiota was partly correlated to dry eye severity. |
| first_indexed | 2024-12-14T08:50:07Z |
| format | Article |
| id | doaj.art-b70eaf70a714434c85662aaea4b0e1c2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:50:07Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-b70eaf70a714434c85662aaea4b0e1c22022-12-21T23:09:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01152e022902910.1371/journal.pone.0229029Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity.Jayoon MoonSe Hyun ChoiChang Ho YoonMee Kum KimOBJECTIVE:To investigate gut dysbiosis in patients with Sjögren's syndrome (SS) or dry eye syndrome (DES) compared to normal subjects and to evaluate the association of dysbiosis with dry eye severity. METHODS:10 subjects with SS, 14 subjects with DES and 12 controls were enrolled. Corneal staining, tear break up time (TBUT) and tear secretion were evaluated. Bacterial genomic 16s rRNA from stool samples were analyzed. Main outcomes were microbiome compositional differences among groups and their correlation to dry eye signs. RESULTS:Gut microbiome analysis revealed significant compositional differences in SS compared to controls and DES. In phylum, Bacteriodetes increased, while Firmicutes/Bacteroidetes ratio and Actinobacteria decreased (p<0.05). In genus, Bifidobacterium was reduced (vs controls; p = 0.025, vs DES; p = 0.026). Beta diversity of SS also showed significant distances from controls and DES (p = 0.007 and 0.019, respectively). SS showed decreased genus of Blautia (p = 0.041), Dorea (p = 0.025) and Agathobacter (p = 0.035) compared to controls and increased genus of Prevotella (p = 0.026), Odoribacter (p = 0.028) and Alistipes (p = 0.46) compared to DES. On the other hand, DES only had increased genus Veillonella (p = 0.045) and reduced Subdoligranulum (p = 0.035) compared to controls. Bacteroidetes, Actinobacteria and Bifidobacterium were significantly related with dry eye signs (p<0.05). After adjustment of age, gender and group classification, multivariate linear regression analysis revealed tear secretion was strongly affected by Prevotella (p = 0.025). With additional adjustment of hydroxychloroquine use, TBUT was markedly affected by Prevotella (p = 0.037) and Actinobacteria (p = 0.001). CONCLUSIONS:Sjögren's syndrome showed significant gut dysbiosis compared to controls and environmental dry eye syndrome, while dry eye patients showed compositional changes of gut microbiome somewhere in between Sjögren's syndrome and controls. Dysbiosis of the gut microbiota was partly correlated to dry eye severity.https://doi.org/10.1371/journal.pone.0229029 |
| spellingShingle | Jayoon Moon Se Hyun Choi Chang Ho Yoon Mee Kum Kim Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity. PLoS ONE |
| title | Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity. |
| title_full | Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity. |
| title_fullStr | Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity. |
| title_full_unstemmed | Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity. |
| title_short | Gut dysbiosis is prevailing in Sjögren's syndrome and is related to dry eye severity. |
| title_sort | gut dysbiosis is prevailing in sjogren s syndrome and is related to dry eye severity |
| url | https://doi.org/10.1371/journal.pone.0229029 |
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