Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance
Abstract Background Fatty acid desaturases (FADs) are involved in regulating plant fatty acid composition by adding double bonds to growing hydrocarbon chain. Apart from regulating fatty acid composition FADs are of great importance, and are involved in stress responsiveness, plant development, and...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04232-9 |
| _version_ | 1827948217162006528 |
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| author | Nabeel Shaheen Uzair Muhammad Khan Ayesha Farooq Ummul Buneen Zafar Sultan Habibullah Khan Shakeel Ahmad Muhammad Tehseen Azhar Rana Muhammad Atif Iqrar Ahmad Rana Hyojin Seo |
| author_facet | Nabeel Shaheen Uzair Muhammad Khan Ayesha Farooq Ummul Buneen Zafar Sultan Habibullah Khan Shakeel Ahmad Muhammad Tehseen Azhar Rana Muhammad Atif Iqrar Ahmad Rana Hyojin Seo |
| author_sort | Nabeel Shaheen |
| collection | DOAJ |
| description | Abstract Background Fatty acid desaturases (FADs) are involved in regulating plant fatty acid composition by adding double bonds to growing hydrocarbon chain. Apart from regulating fatty acid composition FADs are of great importance, and are involved in stress responsiveness, plant development, and defense mechanisms. FADs have been extensively studied in crop plants, and are broadly classed into soluble and non-soluble fatty acids. However, FADs have not yet been characterized in Brassica carinata and its progenitors. Results Here we have performed comparative genome-wide identification of FADs and have identified 131 soluble and 28 non-soluble FADs in allotetraploid B. carinata and its diploid parents. Most soluble FAD proteins are predicted to be resided in endomembrane system, whereas FAB proteins were found to be localized in chloroplast. Phylogenetic analysis classed the soluble and non-soluble FAD proteins into seven and four clusters, respectively. Positive type of selection seemed to be dominant in both FADs suggesting the impact of evolution on these gene families. Upstream regions of both FADs were enriched in stress related cis-regulatory elements and among them ABRE type of elements were in abundance. Comparative transcriptomic data analysis output highlighted that FADs expression reduced gradually in mature seed and embryonic tissues. Moreover, under heat stress during seed and embryo development seven genes remained up-regulated regardless of external stress. Three FADs were only induced under elevated temperature whereas five genes were upregulated under Xanthomonas campestris stress suggesting their involvement in abiotic and biotic stress response. Conclusions The current study provides insights into the evolution of FADs and their role in B. carinata under stress conditions. Moreover, the functional characterization of stress-related genes would exploit their utilization in future breeding programs of B. carinata and its progenitors. |
| first_indexed | 2024-04-09T12:50:50Z |
| format | Article |
| id | doaj.art-b7103ffde39141f8af26e475c9801e4d |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-09T12:50:50Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-b7103ffde39141f8af26e475c9801e4d2023-05-14T11:12:51ZengBMCBMC Plant Biology1471-22292023-05-0123111310.1186/s12870-023-04232-9Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress toleranceNabeel Shaheen0Uzair Muhammad Khan1Ayesha Farooq2Ummul Buneen Zafar3Sultan Habibullah Khan4Shakeel Ahmad5Muhammad Tehseen Azhar6Rana Muhammad Atif7Iqrar Ahmad Rana8Hyojin Seo9Department of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureCenter for Advanced Studies in Agriculture and Food security, University of AgricultureCenter for Advanced Studies in Agriculture and Food security, University of AgricultureSeed Center and Plant Genetic Resources Bank, Ministry of Environment, Water & AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureCenter for Advanced Studies in Agriculture and Food security, University of AgricultureKorea Soybean Research InstituteAbstract Background Fatty acid desaturases (FADs) are involved in regulating plant fatty acid composition by adding double bonds to growing hydrocarbon chain. Apart from regulating fatty acid composition FADs are of great importance, and are involved in stress responsiveness, plant development, and defense mechanisms. FADs have been extensively studied in crop plants, and are broadly classed into soluble and non-soluble fatty acids. However, FADs have not yet been characterized in Brassica carinata and its progenitors. Results Here we have performed comparative genome-wide identification of FADs and have identified 131 soluble and 28 non-soluble FADs in allotetraploid B. carinata and its diploid parents. Most soluble FAD proteins are predicted to be resided in endomembrane system, whereas FAB proteins were found to be localized in chloroplast. Phylogenetic analysis classed the soluble and non-soluble FAD proteins into seven and four clusters, respectively. Positive type of selection seemed to be dominant in both FADs suggesting the impact of evolution on these gene families. Upstream regions of both FADs were enriched in stress related cis-regulatory elements and among them ABRE type of elements were in abundance. Comparative transcriptomic data analysis output highlighted that FADs expression reduced gradually in mature seed and embryonic tissues. Moreover, under heat stress during seed and embryo development seven genes remained up-regulated regardless of external stress. Three FADs were only induced under elevated temperature whereas five genes were upregulated under Xanthomonas campestris stress suggesting their involvement in abiotic and biotic stress response. Conclusions The current study provides insights into the evolution of FADs and their role in B. carinata under stress conditions. Moreover, the functional characterization of stress-related genes would exploit their utilization in future breeding programs of B. carinata and its progenitors.https://doi.org/10.1186/s12870-023-04232-9Fatty acid desaturaseB. carinataGenome wide identificationPhylogeneticTranscriptomic data analysis |
| spellingShingle | Nabeel Shaheen Uzair Muhammad Khan Ayesha Farooq Ummul Buneen Zafar Sultan Habibullah Khan Shakeel Ahmad Muhammad Tehseen Azhar Rana Muhammad Atif Iqrar Ahmad Rana Hyojin Seo Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance BMC Plant Biology Fatty acid desaturase B. carinata Genome wide identification Phylogenetic Transcriptomic data analysis |
| title | Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance |
| title_full | Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance |
| title_fullStr | Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance |
| title_full_unstemmed | Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance |
| title_short | Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance |
| title_sort | comparative transcriptomic and evolutionary analysis of fad like genes of brassica species revealed their role in fatty acid biosynthesis and stress tolerance |
| topic | Fatty acid desaturase B. carinata Genome wide identification Phylogenetic Transcriptomic data analysis |
| url | https://doi.org/10.1186/s12870-023-04232-9 |
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