T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis
The aim of the present study was to evaluate the impact of SEMA4A genetic variants on expression of sema4A protein and its relation to autoimmunity development in Systemic Lupus Erythematosus and Rheumatoid Arthritis patients. A total of 541 SLE patients, 390 RA patients and 607 healthy individuals...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-02-01
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| Series: | Autoimmunity |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08916934.2019.1704273 |
| _version_ | 1827817450948788224 |
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| author | Catarina Addobbati Jordão Cavalcanti Vanessa Germoglio Jaqueline de Azevêdo Silva Nadine Glesse Priscila Vianna Giovana Cechim Odirlei Andre Monticielo Ricardo Machado Xavier João Carlos Tavares Brenol Claiton Viegas Brenol Thiago Sotero Fragoso Alexandre Domingues Barbosa Ângela Luiza Branco Pinto Duarte Renê Donizeti Ribeiro Oliveira Paulo Louzada-Júnior Eduardo Antônio Donadi José Artur Bogo Chies Sergio Crovella Paula Sandrin-Garcia |
| author_facet | Catarina Addobbati Jordão Cavalcanti Vanessa Germoglio Jaqueline de Azevêdo Silva Nadine Glesse Priscila Vianna Giovana Cechim Odirlei Andre Monticielo Ricardo Machado Xavier João Carlos Tavares Brenol Claiton Viegas Brenol Thiago Sotero Fragoso Alexandre Domingues Barbosa Ângela Luiza Branco Pinto Duarte Renê Donizeti Ribeiro Oliveira Paulo Louzada-Júnior Eduardo Antônio Donadi José Artur Bogo Chies Sergio Crovella Paula Sandrin-Garcia |
| author_sort | Catarina Addobbati Jordão Cavalcanti |
| collection | DOAJ |
| description | The aim of the present study was to evaluate the impact of SEMA4A genetic variants on expression of sema4A protein and its relation to autoimmunity development in Systemic Lupus Erythematosus and Rheumatoid Arthritis patients. A total of 541 SLE patients, 390 RA patients and 607 healthy individuals were genotyped. We also assessed SEMA4A mRNA expression from whole blood cells and the in vitro protein production from resting and activated T lymphocytes as well as mature dendritic cells from healthy individuals stratified according to their genotypes for SLE/RA associated SEMA4A variants. Our results showed that T/T genotype for rs3738581 SNP is associated with both RA and SLE development (p = .000053, OR = 2.35; p = .0019, OR = 2.07, respectively; statistical power = 100%) and also to an increased in vitro sema4A production in active T lymphocytes. Our findings are indicative of a T cell-specific upregulation of sema4A in the presence of T/T genotype, being a risk factor for SLE and RA. |
| first_indexed | 2024-03-12T00:34:25Z |
| format | Article |
| id | doaj.art-b710504c7eb64163b8ae7fe6126d6183 |
| institution | Directory Open Access Journal |
| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-12T00:34:25Z |
| publishDate | 2020-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj.art-b710504c7eb64163b8ae7fe6126d61832023-09-15T10:01:08ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2020-02-01532657010.1080/08916934.2019.17042731704273T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritisCatarina Addobbati Jordão Cavalcanti0Vanessa Germoglio1Jaqueline de Azevêdo Silva2Nadine Glesse3Priscila Vianna4Giovana Cechim5Odirlei Andre Monticielo6Ricardo Machado Xavier7João Carlos Tavares Brenol8Claiton Viegas Brenol9Thiago Sotero Fragoso10Alexandre Domingues Barbosa11Ângela Luiza Branco Pinto Duarte12Renê Donizeti Ribeiro Oliveira13Paulo Louzada-Júnior14Eduardo Antônio Donadi15José Artur Bogo Chies16Sergio Crovella17Paula Sandrin-Garcia18Federal University of PernambucoFederal University of PernambucoFederal University of PernambucoFederal University of Rio Grande do SulFederal University of Rio Grande do SulFederal University of Rio Grande do SulClinical Hospital, Federal University of Rio Grande do SulClinical Hospital, Federal University of Rio Grande do SulClinical Hospital, Federal University of Rio Grande do SulClinical Hospital, Federal University of Rio Grande do SulFederal University of AlagoasFederal University of PernambucoFederal University of PernambucoMedicine Faculty of Ribeirão Preto, University of São PauloMedicine Faculty of Ribeirão Preto, University of São PauloMedicine Faculty of Ribeirão Preto, University of São PauloFederal University of Rio Grande do SulFederal University of PernambucoFederal University of PernambucoThe aim of the present study was to evaluate the impact of SEMA4A genetic variants on expression of sema4A protein and its relation to autoimmunity development in Systemic Lupus Erythematosus and Rheumatoid Arthritis patients. A total of 541 SLE patients, 390 RA patients and 607 healthy individuals were genotyped. We also assessed SEMA4A mRNA expression from whole blood cells and the in vitro protein production from resting and activated T lymphocytes as well as mature dendritic cells from healthy individuals stratified according to their genotypes for SLE/RA associated SEMA4A variants. Our results showed that T/T genotype for rs3738581 SNP is associated with both RA and SLE development (p = .000053, OR = 2.35; p = .0019, OR = 2.07, respectively; statistical power = 100%) and also to an increased in vitro sema4A production in active T lymphocytes. Our findings are indicative of a T cell-specific upregulation of sema4A in the presence of T/T genotype, being a risk factor for SLE and RA.http://dx.doi.org/10.1080/08916934.2019.1704273sema4asystemic lupus erythematosusrheumatoid arthritispolymorphismt cells |
| spellingShingle | Catarina Addobbati Jordão Cavalcanti Vanessa Germoglio Jaqueline de Azevêdo Silva Nadine Glesse Priscila Vianna Giovana Cechim Odirlei Andre Monticielo Ricardo Machado Xavier João Carlos Tavares Brenol Claiton Viegas Brenol Thiago Sotero Fragoso Alexandre Domingues Barbosa Ângela Luiza Branco Pinto Duarte Renê Donizeti Ribeiro Oliveira Paulo Louzada-Júnior Eduardo Antônio Donadi José Artur Bogo Chies Sergio Crovella Paula Sandrin-Garcia T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis Autoimmunity sema4a systemic lupus erythematosus rheumatoid arthritis polymorphism t cells |
| title | T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis |
| title_full | T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis |
| title_fullStr | T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis |
| title_full_unstemmed | T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis |
| title_short | T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis |
| title_sort | t cell specific upregulation of sema4a as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis |
| topic | sema4a systemic lupus erythematosus rheumatoid arthritis polymorphism t cells |
| url | http://dx.doi.org/10.1080/08916934.2019.1704273 |
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