Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins
Crickets are known to be a promising alternative protein source. However, a negative consumer bias and an off-flavor have become obstacles to the use of these insects in the food industry. In this study, we extracted the protein from commercial cricket powder by employing alkaline extraction–acid pr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/21/4032 |
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| author | Edward Thanakorn Wongprasert Thasorn Bunyakanchana Panattida Siripitakpong Kotchakorn Supabowornsathit Tirayut Vilaivan Inthawoot Suppavorasatit |
| author_facet | Edward Thanakorn Wongprasert Thasorn Bunyakanchana Panattida Siripitakpong Kotchakorn Supabowornsathit Tirayut Vilaivan Inthawoot Suppavorasatit |
| author_sort | Edward |
| collection | DOAJ |
| description | Crickets are known to be a promising alternative protein source. However, a negative consumer bias and an off-flavor have become obstacles to the use of these insects in the food industry. In this study, we extracted the protein from commercial cricket powder by employing alkaline extraction–acid precipitation and including ammonium sulfate. The physicochemical and functional properties of the proteins were determined. It was found that, upon including 60% ammonium sulfate, the cricket protein isolate (CPI) had the highest protein content (~94%, <i>w</i>/<i>w</i>). The circular dichroism results indicated that a higher amount of ammonium sulfate drastically changed the secondary structure of the CPI by decreasing its α-helix content and enhancing its surface hydrophobicity. The lowest solubility of CPI was observed at pH 5. The CPI also showed better foaming properties and oil-holding capacity (OHC) compared with the cricket powder. In conclusion, adding ammonium sulfate affected the physicochemical and functional properties of the CPI, allowing it to be used as an alternative protein in protein-enriched foods and beverages. |
| first_indexed | 2024-03-11T11:29:59Z |
| format | Article |
| id | doaj.art-b7108fce106042feb785356e0b47d1f5 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T11:29:59Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-b7108fce106042feb785356e0b47d1f52023-11-10T15:03:08ZengMDPI AGFoods2304-81582023-11-011221403210.3390/foods12214032Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of ProteinsEdward0Thanakorn Wongprasert1Thasorn Bunyakanchana2Panattida Siripitakpong3Kotchakorn Supabowornsathit4Tirayut Vilaivan5Inthawoot Suppavorasatit6Department of Food Technology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Wangmai, Pathumwan, Bangkok 10330, ThailandDepartment of Food Technology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Wangmai, Pathumwan, Bangkok 10330, ThailandDepartment of Food Technology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Wangmai, Pathumwan, Bangkok 10330, ThailandDepartment of Food Technology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Wangmai, Pathumwan, Bangkok 10330, ThailandDepartment of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Wangmai, Pathumwan, Bangkok 10330, ThailandDepartment of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Wangmai, Pathumwan, Bangkok 10330, ThailandDepartment of Food Technology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Wangmai, Pathumwan, Bangkok 10330, ThailandCrickets are known to be a promising alternative protein source. However, a negative consumer bias and an off-flavor have become obstacles to the use of these insects in the food industry. In this study, we extracted the protein from commercial cricket powder by employing alkaline extraction–acid precipitation and including ammonium sulfate. The physicochemical and functional properties of the proteins were determined. It was found that, upon including 60% ammonium sulfate, the cricket protein isolate (CPI) had the highest protein content (~94%, <i>w</i>/<i>w</i>). The circular dichroism results indicated that a higher amount of ammonium sulfate drastically changed the secondary structure of the CPI by decreasing its α-helix content and enhancing its surface hydrophobicity. The lowest solubility of CPI was observed at pH 5. The CPI also showed better foaming properties and oil-holding capacity (OHC) compared with the cricket powder. In conclusion, adding ammonium sulfate affected the physicochemical and functional properties of the CPI, allowing it to be used as an alternative protein in protein-enriched foods and beverages.https://www.mdpi.com/2304-8158/12/21/4032cricketinsectproteinalkaline extractionacid precipitationammonium sulfate |
| spellingShingle | Edward Thanakorn Wongprasert Thasorn Bunyakanchana Panattida Siripitakpong Kotchakorn Supabowornsathit Tirayut Vilaivan Inthawoot Suppavorasatit Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins Foods cricket insect protein alkaline extraction acid precipitation ammonium sulfate |
| title | Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins |
| title_full | Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins |
| title_fullStr | Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins |
| title_full_unstemmed | Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins |
| title_short | Cricket Protein Isolate Extraction: Effect of Ammonium Sulfate on Physicochemical and Functional Properties of Proteins |
| title_sort | cricket protein isolate extraction effect of ammonium sulfate on physicochemical and functional properties of proteins |
| topic | cricket insect protein alkaline extraction acid precipitation ammonium sulfate |
| url | https://www.mdpi.com/2304-8158/12/21/4032 |
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