Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules
Exopolysaccharide-producing cyanobacterial strains in biological soil crusts are described, in addition to their chemical properties and antioxidant and flocculation activities. The EPSs from Pudukkottai blackish biological soil crusts (PBBSCs) showed significant amounts of total soluble proteins (0...
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MDPI AG
2023-07-01
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| author | Mani Vinoth Sivaprakasam Sivasankari Abdul Kareem Khaleel Ahamed Khawla Ibrahim Alsamhary Nouf Mohammed Al-enazi Neveen Abdel-Raouf Reem Mohammed Alharbi Rasiravathanahalli Kaveriyappan Govindarajan Gangalla Ravi Khaloud Mohammed Alarjani Essam N. Sholkamy |
| author_facet | Mani Vinoth Sivaprakasam Sivasankari Abdul Kareem Khaleel Ahamed Khawla Ibrahim Alsamhary Nouf Mohammed Al-enazi Neveen Abdel-Raouf Reem Mohammed Alharbi Rasiravathanahalli Kaveriyappan Govindarajan Gangalla Ravi Khaloud Mohammed Alarjani Essam N. Sholkamy |
| author_sort | Mani Vinoth |
| collection | DOAJ |
| description | Exopolysaccharide-producing cyanobacterial strains in biological soil crusts are described, in addition to their chemical properties and antioxidant and flocculation activities. The EPSs from Pudukkottai blackish biological soil crusts (PBBSCs) showed significant amounts of total soluble proteins (0.1687 mg/mL) and carbohydrates (0.8056 mg/mL) compared with the Ariyalur blackish biological soil crusts (ABBSCs). LC-MS analysis of the cyanobacterial polysaccharides revealed the presence of natural sugars such as ribose and glucose/mannose, and uronic acids. The FTIR spectrum showed specific peak for OH and –NH stretching, C–H stretching, and carboxylic acids as the dominant groups in EPS. The in vitro DPPH assay of EPSs from PBBSCs showed 74.3% scavenging activity. Furthermore, the reducing power was determined to be 0.59 ata 500 mg/mL concentration, respectively. The extracted EPSs from the biological soil crust flocculated Kaolin clay suspension maximum at 500 mg/mL. Consequently, the cyanobacterial strain and exopolysaccharide characterization from the sacred forest’s biological soil crust were analyzed for their bioactive potential, bio-crust diversity, and distribution. |
| first_indexed | 2024-03-09T10:56:34Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T10:56:34Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
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| spelling | doaj.art-b710c00675a34805b6ab29f99902f9542023-12-01T01:38:10ZengMDPI AGBiology2079-77372023-07-01128106510.3390/biology12081065Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal BiomoleculesMani Vinoth0Sivaprakasam Sivasankari1Abdul Kareem Khaleel Ahamed2Khawla Ibrahim Alsamhary3Nouf Mohammed Al-enazi4Neveen Abdel-Raouf5Reem Mohammed Alharbi6Rasiravathanahalli Kaveriyappan Govindarajan7Gangalla Ravi8Khaloud Mohammed Alarjani9Essam N. Sholkamy10PG and Research Department of Botany, Jamal Mohamed College, Tiruchirappalli 620020, Tamil Nadu, IndiaDepartment of Biology, Periyar Government Arts College, Cuddalore 607001, Tamil Nadu, IndiaPG and Research Department of Botany, Jamal Mohamed College, Tiruchirappalli 620020, Tamil Nadu, IndiaDepartmen of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartmen of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartmen of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaBiology Department, Science College, University of Hafr Al Batin, Hafr Al Batin 39524, Saudi ArabiaDepartment of Biotechnology, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaDepartment of Microbiology, Kakatiya University, Warangal 506009, Telangana, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaExopolysaccharide-producing cyanobacterial strains in biological soil crusts are described, in addition to their chemical properties and antioxidant and flocculation activities. The EPSs from Pudukkottai blackish biological soil crusts (PBBSCs) showed significant amounts of total soluble proteins (0.1687 mg/mL) and carbohydrates (0.8056 mg/mL) compared with the Ariyalur blackish biological soil crusts (ABBSCs). LC-MS analysis of the cyanobacterial polysaccharides revealed the presence of natural sugars such as ribose and glucose/mannose, and uronic acids. The FTIR spectrum showed specific peak for OH and –NH stretching, C–H stretching, and carboxylic acids as the dominant groups in EPS. The in vitro DPPH assay of EPSs from PBBSCs showed 74.3% scavenging activity. Furthermore, the reducing power was determined to be 0.59 ata 500 mg/mL concentration, respectively. The extracted EPSs from the biological soil crust flocculated Kaolin clay suspension maximum at 500 mg/mL. Consequently, the cyanobacterial strain and exopolysaccharide characterization from the sacred forest’s biological soil crust were analyzed for their bioactive potential, bio-crust diversity, and distribution.https://www.mdpi.com/2079-7737/12/8/1065biological soil crustcyanobacteriaantioxidantexopolysaccharideflocculation |
| spellingShingle | Mani Vinoth Sivaprakasam Sivasankari Abdul Kareem Khaleel Ahamed Khawla Ibrahim Alsamhary Nouf Mohammed Al-enazi Neveen Abdel-Raouf Reem Mohammed Alharbi Rasiravathanahalli Kaveriyappan Govindarajan Gangalla Ravi Khaloud Mohammed Alarjani Essam N. Sholkamy Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules Biology biological soil crust cyanobacteria antioxidant exopolysaccharide flocculation |
| title | Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules |
| title_full | Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules |
| title_fullStr | Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules |
| title_full_unstemmed | Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules |
| title_short | Bio-Characterization and Liquid Chromatography–Mass Spectrometry Analysis of Exopolysaccharides in Biofilm-Producing Cyanobacteria Isolated from Soil Crust: Exploring the Potential of Microalgal Biomolecules |
| title_sort | bio characterization and liquid chromatography mass spectrometry analysis of exopolysaccharides in biofilm producing cyanobacteria isolated from soil crust exploring the potential of microalgal biomolecules |
| topic | biological soil crust cyanobacteria antioxidant exopolysaccharide flocculation |
| url | https://www.mdpi.com/2079-7737/12/8/1065 |
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