Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer
Abstract Background With the increasing incidence of colorectal cancer (CRC), its accurate diagnosis is critical and in high demand. However, conventional methods are not ideal due to invasiveness and low accuracy. Herein, we aimed to identify efficient CRC mRNA markers in a non-invasive manner usin...
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13036-020-0225-9 |
| _version_ | 1818971177894281216 |
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| author | Byung Seok Cha Ki Soo Park Jun Seok Park |
| author_facet | Byung Seok Cha Ki Soo Park Jun Seok Park |
| author_sort | Byung Seok Cha |
| collection | DOAJ |
| description | Abstract Background With the increasing incidence of colorectal cancer (CRC), its accurate diagnosis is critical and in high demand. However, conventional methods are not ideal due to invasiveness and low accuracy. Herein, we aimed to identify efficient CRC mRNA markers in a non-invasive manner using CRC-derived extracellular vesicles (EVs). The expression levels of EV mRNAs from cancer cell lines were compared with those of a normal cell line using quantitative polymerase chain reaction. Eight markers were evaluated in plasma EVs from CRC patients and healthy controls. The diagnostic value of each marker, individually or in combination, was then determined using recessive operating characteristics analyses and the Mann-Whitney U test. Results Eight mRNA markers (MYC, VEGF, CDX2, CD133, CEA, CK19, EpCAM, and CD24) were found to be more abundant in EVs derived from cancer cell lines compared to control cell lines. A combination of VEGF and CD133 showed the highest sensitivity (100%), specificity (80%), and accuracy (93%) and an area under the curve of 0.96; hence, these markers were deemed to be the CRC signature. Moreover, this signature was found to be highly expressed in CRC-derived EVs compared to healthy controls. Conclusions VEGF and CD133 mRNAs comprise a unique CRC signature in EVs that has the potential to act as a novel, non-invasive, and accurate biomarker that would improve the current diagnostic platform for CRC, while also serving to strengthen the value of EV mRNA as diagnostic markers for myriad of diseases. |
| first_indexed | 2024-12-20T14:48:14Z |
| format | Article |
| id | doaj.art-b711ea63619947bf826881cdef79a01f |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-12-20T14:48:14Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-b711ea63619947bf826881cdef79a01f2022-12-21T19:37:04ZengBMCJournal of Biological Engineering1754-16112020-02-011411910.1186/s13036-020-0225-9Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancerByung Seok Cha0Ki Soo Park1Jun Seok Park2Department of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Biological Engineering, College of Engineering, Konkuk UniversitySchool of Medicine, Kyungpook National UniversityAbstract Background With the increasing incidence of colorectal cancer (CRC), its accurate diagnosis is critical and in high demand. However, conventional methods are not ideal due to invasiveness and low accuracy. Herein, we aimed to identify efficient CRC mRNA markers in a non-invasive manner using CRC-derived extracellular vesicles (EVs). The expression levels of EV mRNAs from cancer cell lines were compared with those of a normal cell line using quantitative polymerase chain reaction. Eight markers were evaluated in plasma EVs from CRC patients and healthy controls. The diagnostic value of each marker, individually or in combination, was then determined using recessive operating characteristics analyses and the Mann-Whitney U test. Results Eight mRNA markers (MYC, VEGF, CDX2, CD133, CEA, CK19, EpCAM, and CD24) were found to be more abundant in EVs derived from cancer cell lines compared to control cell lines. A combination of VEGF and CD133 showed the highest sensitivity (100%), specificity (80%), and accuracy (93%) and an area under the curve of 0.96; hence, these markers were deemed to be the CRC signature. Moreover, this signature was found to be highly expressed in CRC-derived EVs compared to healthy controls. Conclusions VEGF and CD133 mRNAs comprise a unique CRC signature in EVs that has the potential to act as a novel, non-invasive, and accurate biomarker that would improve the current diagnostic platform for CRC, while also serving to strengthen the value of EV mRNA as diagnostic markers for myriad of diseases.https://doi.org/10.1186/s13036-020-0225-9Colorectal cancerExtracellular vesiclemRNAVEGFCD133Non-invasive biomarker |
| spellingShingle | Byung Seok Cha Ki Soo Park Jun Seok Park Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer Journal of Biological Engineering Colorectal cancer Extracellular vesicle mRNA VEGF CD133 Non-invasive biomarker |
| title | Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer |
| title_full | Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer |
| title_fullStr | Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer |
| title_full_unstemmed | Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer |
| title_short | Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer |
| title_sort | signature mrna markers in extracellular vesicles for the accurate diagnosis of colorectal cancer |
| topic | Colorectal cancer Extracellular vesicle mRNA VEGF CD133 Non-invasive biomarker |
| url | https://doi.org/10.1186/s13036-020-0225-9 |
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