The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride
Zhenbao pill (ZBP) is a type of traditional Mongolian medicine that contains 241 ingredients such as flavonoids, anthraquinones, triterpenoids, sterols, and Calculus bovis, which are mainly used for treating neurological diseases in clinical practice. In this study, we established an Alzheimer’s dis...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223001877 |
| _version_ | 1797894756145037312 |
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| author | Hongsong Chen Huilei Li Xiaoyu Yin Yuanyuan Liu Tengdan Zhang Hui Wu Guiying Kang Yongli Yu Meirong Bai Liming Bao Jingfeng Yang Wu Dong |
| author_facet | Hongsong Chen Huilei Li Xiaoyu Yin Yuanyuan Liu Tengdan Zhang Hui Wu Guiying Kang Yongli Yu Meirong Bai Liming Bao Jingfeng Yang Wu Dong |
| author_sort | Hongsong Chen |
| collection | DOAJ |
| description | Zhenbao pill (ZBP) is a type of traditional Mongolian medicine that contains 241 ingredients such as flavonoids, anthraquinones, triterpenoids, sterols, and Calculus bovis, which are mainly used for treating neurological diseases in clinical practice. In this study, we established an Alzheimer’s disease (AD)-mimetic symptomatic zebrafish model by AlCl3 treatment and investigated the therapeutic effect of ZBP on the AD-mimetic zebrafish model. The results revealed that AlCl3 treatment led to behavioral impairment, induced apoptotic cell (body) in the brain, increased acetylcholinesterase (AChE) activity, and decreased the ACh content in zebrafish. After treatment with ZBP, 16 μg/mL ZBP could alleviate the behavioral disorder of zebrafish larvae. This effect was found superior to the effect of the cholinesterase inhibitor donepezil hydrochloride. The analysis of transcriptomics data revealed that ZBP mainly acted on apoptosis, the p53 signaling pathway, and the enrichment of the AChE-associated pathway. Furthermore, the overexpression of AChE RNA produced similar AD-mimetic disorders, whereas AChE-gene knockdown (AChE-MO) decreased the AChE activity and alleviated the AD-mimetic symptoms. These results suggested that ZBP is a potential cholinergic-enhancing drug, which can be used for the treatment of AD in the future. |
| first_indexed | 2024-04-10T07:14:23Z |
| format | Article |
| id | doaj.art-b713639ea97342a3a78d1c835cd79317 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-10T07:14:23Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-b713639ea97342a3a78d1c835cd793172023-02-26T04:26:44ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-04-01160114399The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chlorideHongsong Chen0Huilei Li1Xiaoyu Yin2Yuanyuan Liu3Tengdan Zhang4Hui Wu5Guiying Kang6Yongli Yu7Meirong Bai8Liming Bao9Jingfeng Yang10Wu Dong11Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, ChinaAffiliated Hospital of Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China; Corresponding authors.Graduate School of Engineering, Muroran Institute of Technology, Muroran 050-8585, JapanInner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, ChinaInner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, ChinaInner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, ChinaInner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, ChinaCollege of Life Science and Food Science, Inner Mongolia Minzu University, Tongliao 028000, ChinaKey Laboratory of Mongolian Medicine Research and Development Engineering, Ministry of Education, The Medical College of Inner Mongolia Minzu University, 028000 Tongliao, Inner Mongolia, ChinaKey Laboratory of Mongolian Medicine Research and Development Engineering, Ministry of Education, The Medical College of Inner Mongolia Minzu University, 028000 Tongliao, Inner Mongolia, ChinaInner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, ChinaInner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China; Corresponding authors.Zhenbao pill (ZBP) is a type of traditional Mongolian medicine that contains 241 ingredients such as flavonoids, anthraquinones, triterpenoids, sterols, and Calculus bovis, which are mainly used for treating neurological diseases in clinical practice. In this study, we established an Alzheimer’s disease (AD)-mimetic symptomatic zebrafish model by AlCl3 treatment and investigated the therapeutic effect of ZBP on the AD-mimetic zebrafish model. The results revealed that AlCl3 treatment led to behavioral impairment, induced apoptotic cell (body) in the brain, increased acetylcholinesterase (AChE) activity, and decreased the ACh content in zebrafish. After treatment with ZBP, 16 μg/mL ZBP could alleviate the behavioral disorder of zebrafish larvae. This effect was found superior to the effect of the cholinesterase inhibitor donepezil hydrochloride. The analysis of transcriptomics data revealed that ZBP mainly acted on apoptosis, the p53 signaling pathway, and the enrichment of the AChE-associated pathway. Furthermore, the overexpression of AChE RNA produced similar AD-mimetic disorders, whereas AChE-gene knockdown (AChE-MO) decreased the AChE activity and alleviated the AD-mimetic symptoms. These results suggested that ZBP is a potential cholinergic-enhancing drug, which can be used for the treatment of AD in the future.http://www.sciencedirect.com/science/article/pii/S0753332223001877Alzheimer's diseaseZhenbao pillZebrafishApoptosisAcetylcholinesterase |
| spellingShingle | Hongsong Chen Huilei Li Xiaoyu Yin Yuanyuan Liu Tengdan Zhang Hui Wu Guiying Kang Yongli Yu Meirong Bai Liming Bao Jingfeng Yang Wu Dong The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride Biomedicine & Pharmacotherapy Alzheimer's disease Zhenbao pill Zebrafish Apoptosis Acetylcholinesterase |
| title | The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride |
| title_full | The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride |
| title_fullStr | The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride |
| title_full_unstemmed | The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride |
| title_short | The therapeutic effect of Zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride |
| title_sort | therapeutic effect of zhenbao pills on behavioral changes in zebrafish caused by aluminum chloride |
| topic | Alzheimer's disease Zhenbao pill Zebrafish Apoptosis Acetylcholinesterase |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223001877 |
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