Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols
Tracheal replacement with a bioengineered tracheal substitute has been developed for long-segment tracheal diseases. The decellularized tracheal scaffold is an alternative for cell seeding. It is not defined if the storage scaffold produces changes in the scaffold’s biomechanical properties. We test...
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MDPI AG
2023-05-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/5/584 |
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| author | Alberto Bruning Guimaraes Aristides Tadeu Correia Ronaldo Soares da Silva Elizabete Silva dos Santos Natalia de Souza Xavier Costa Marisa Dolhnikoff Marina Maizato Idagene Aparecida Cestari Paulo Manuel Pego-Fernandes Paulo Francisco Guerreiro Cardoso |
| author_facet | Alberto Bruning Guimaraes Aristides Tadeu Correia Ronaldo Soares da Silva Elizabete Silva dos Santos Natalia de Souza Xavier Costa Marisa Dolhnikoff Marina Maizato Idagene Aparecida Cestari Paulo Manuel Pego-Fernandes Paulo Francisco Guerreiro Cardoso |
| author_sort | Alberto Bruning Guimaraes |
| collection | DOAJ |
| description | Tracheal replacement with a bioengineered tracheal substitute has been developed for long-segment tracheal diseases. The decellularized tracheal scaffold is an alternative for cell seeding. It is not defined if the storage scaffold produces changes in the scaffold’s biomechanical properties. We tested three protocols for porcine tracheal scaffold preservation immersed in PBS and alcohol 70%, in the fridge and under cryopreservation. Ninety-six porcine tracheas (12 in natura, 84 decellularized) were divided into three groups (PBS, alcohol, and cryopreservation). Twelve tracheas were analyzed after three and six months. The assessment included residual DNA, cytotoxicity, collagen contents, and mechanical properties. Decellularization increased the maximum load and stress in the longitudinal axis and decreased the maximum load in the transverse axis. The decellularization of the porcine trachea produced structurally viable scaffolds, with a preserved collagen matrix suitable for further bioengineering. Despite the cyclic washings, the scaffolds remained cytotoxic. The comparison of the storage protocols (PBS at 4 °C, alcohol at 4 °C, and slow cooling cryopreservation with cryoprotectants) showed no significant differences in the amount of collagen and in the biomechanical properties of the scaffolds. Storage in PBS solution at 4 °C for six months did not change the scaffold mechanics. |
| first_indexed | 2024-03-11T03:56:02Z |
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| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T03:56:02Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-b7159f9b6cca42b1b8ddbc22afa76fc92023-11-18T00:31:39ZengMDPI AGBioengineering2306-53542023-05-0110558410.3390/bioengineering10050584Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different ProtocolsAlberto Bruning Guimaraes0Aristides Tadeu Correia1Ronaldo Soares da Silva2Elizabete Silva dos Santos3Natalia de Souza Xavier Costa4Marisa Dolhnikoff5Marina Maizato6Idagene Aparecida Cestari7Paulo Manuel Pego-Fernandes8Paulo Francisco Guerreiro Cardoso9Organ and Tissue Laboratory, LIM 61, Division of Thoracic Surgery, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilOrgan and Tissue Laboratory, LIM 61, Division of Thoracic Surgery, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilOrgan and Tissue Laboratory, LIM 61, Division of Thoracic Surgery, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilOrgan and Tissue Laboratory, LIM 61, Division of Thoracic Surgery, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilLaboratorio de Poluicao Atmosferica Experimental (LIM05), Departamento de Patologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo 01246-000, BrazilLaboratorio de Poluicao Atmosferica Experimental (LIM05), Departamento de Patologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo 01246-000, BrazilBioengenharia, Instituto do Coração do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilBioengenharia, Instituto do Coração do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilOrgan and Tissue Laboratory, LIM 61, Division of Thoracic Surgery, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilOrgan and Tissue Laboratory, LIM 61, Division of Thoracic Surgery, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 05403-904, BrazilTracheal replacement with a bioengineered tracheal substitute has been developed for long-segment tracheal diseases. The decellularized tracheal scaffold is an alternative for cell seeding. It is not defined if the storage scaffold produces changes in the scaffold’s biomechanical properties. We tested three protocols for porcine tracheal scaffold preservation immersed in PBS and alcohol 70%, in the fridge and under cryopreservation. Ninety-six porcine tracheas (12 in natura, 84 decellularized) were divided into three groups (PBS, alcohol, and cryopreservation). Twelve tracheas were analyzed after three and six months. The assessment included residual DNA, cytotoxicity, collagen contents, and mechanical properties. Decellularization increased the maximum load and stress in the longitudinal axis and decreased the maximum load in the transverse axis. The decellularization of the porcine trachea produced structurally viable scaffolds, with a preserved collagen matrix suitable for further bioengineering. Despite the cyclic washings, the scaffolds remained cytotoxic. The comparison of the storage protocols (PBS at 4 °C, alcohol at 4 °C, and slow cooling cryopreservation with cryoprotectants) showed no significant differences in the amount of collagen and in the biomechanical properties of the scaffolds. Storage in PBS solution at 4 °C for six months did not change the scaffold mechanics.https://www.mdpi.com/2306-5354/10/5/584transplantationtracheabioengineeringtracheal stenosisregenerative medicinebiomechanical phenomena |
| spellingShingle | Alberto Bruning Guimaraes Aristides Tadeu Correia Ronaldo Soares da Silva Elizabete Silva dos Santos Natalia de Souza Xavier Costa Marisa Dolhnikoff Marina Maizato Idagene Aparecida Cestari Paulo Manuel Pego-Fernandes Paulo Francisco Guerreiro Cardoso Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols Bioengineering transplantation trachea bioengineering tracheal stenosis regenerative medicine biomechanical phenomena |
| title | Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols |
| title_full | Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols |
| title_fullStr | Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols |
| title_full_unstemmed | Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols |
| title_short | Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols |
| title_sort | evaluation of structural viability of porcine tracheal scaffolds after 3 and 6 months of storage under three different protocols |
| topic | transplantation trachea bioengineering tracheal stenosis regenerative medicine biomechanical phenomena |
| url | https://www.mdpi.com/2306-5354/10/5/584 |
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