Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection
Abstract Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for tran...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-01587-7 |
| _version_ | 1827603635674021888 |
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| author | Arnab Purohit Sanatan Ghosh Shreeparna Ganguly Madan Singh Negi Shashi Bhushan Tripathi Rituparna Kundu Chaudhuri Dipankar Chakraborti |
| author_facet | Arnab Purohit Sanatan Ghosh Shreeparna Ganguly Madan Singh Negi Shashi Bhushan Tripathi Rituparna Kundu Chaudhuri Dipankar Chakraborti |
| author_sort | Arnab Purohit |
| collection | DOAJ |
| description | Abstract Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for transcriptomic profiling to understand defense signaling reactions that interplay at early stage of this plant–pathogen encounter. Differential transcriptomic profiles were generated through cDNA-AFLP from M1 inoculated resistant and susceptible pigeonpea root tissues. Twenty five percent of transcript derived fragments (TDFs) were found to be pathogen induced. Among them 73 TDFs were re-amplified and sequenced. Homology search of the TDFs in available databases and thorough study of scientific literature identified several pathways, which could play crucial role in defense responses of the F. udum inoculated resistant plants. Some of the defense responsive pathways identified to be active during this interaction are, jasmonic acid and salicylic acid mediated defense responses, cell wall remodeling, vascular development and pattering, abscisic acid mediated responses, effector triggered immunity, and reactive oxygen species mediated signaling. This study identified important wilt responsive regulatory pathways in pigeonpea which will be helpful for further exploration of these resistant components for pigeonpea improvement. |
| first_indexed | 2024-03-09T05:43:29Z |
| format | Article |
| id | doaj.art-b71818bd94b044c1a8ffbc063ee04fdc |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T05:43:29Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b71818bd94b044c1a8ffbc063ee04fdc2023-12-03T12:23:42ZengNature PortfolioScientific Reports2045-23222021-11-0111111710.1038/s41598-021-01587-7Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infectionArnab Purohit0Sanatan Ghosh1Shreeparna Ganguly2Madan Singh Negi3Shashi Bhushan Tripathi4Rituparna Kundu Chaudhuri5Dipankar Chakraborti6Department of Biotechnology, St. Xavier’s College (Autonomous)Department of Genetics, University of CalcuttaDepartment of Biotechnology, St. Xavier’s College (Autonomous)Sustainable Agriculture Division, TERITERI-School of Advanced StudiesDepartment of Botany, Krishnagar Govt. CollegeDepartment of Genetics, University of CalcuttaAbstract Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for transcriptomic profiling to understand defense signaling reactions that interplay at early stage of this plant–pathogen encounter. Differential transcriptomic profiles were generated through cDNA-AFLP from M1 inoculated resistant and susceptible pigeonpea root tissues. Twenty five percent of transcript derived fragments (TDFs) were found to be pathogen induced. Among them 73 TDFs were re-amplified and sequenced. Homology search of the TDFs in available databases and thorough study of scientific literature identified several pathways, which could play crucial role in defense responses of the F. udum inoculated resistant plants. Some of the defense responsive pathways identified to be active during this interaction are, jasmonic acid and salicylic acid mediated defense responses, cell wall remodeling, vascular development and pattering, abscisic acid mediated responses, effector triggered immunity, and reactive oxygen species mediated signaling. This study identified important wilt responsive regulatory pathways in pigeonpea which will be helpful for further exploration of these resistant components for pigeonpea improvement.https://doi.org/10.1038/s41598-021-01587-7 |
| spellingShingle | Arnab Purohit Sanatan Ghosh Shreeparna Ganguly Madan Singh Negi Shashi Bhushan Tripathi Rituparna Kundu Chaudhuri Dipankar Chakraborti Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection Scientific Reports |
| title | Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection |
| title_full | Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection |
| title_fullStr | Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection |
| title_full_unstemmed | Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection |
| title_short | Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection |
| title_sort | comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during fusarium udum infection |
| url | https://doi.org/10.1038/s41598-021-01587-7 |
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