Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls
The aim of the study was to characterize nucleotide sequence polymorphisms in the bovine PTPRQ gene, to search for their possible effect on the expression of myogenic factor 6 (MYF6; MRF4) and myogenic factor 5 (MYF5) genes in skeletal muscle and on meat production traits. Three novel SNPs were foun...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2016-05-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201605-0005_effects-of-polymorphism-in-the-bovine-ptprq-gene-on-the-expression-of-myf6-and-myf5-genes-in-skeletal-muscle-an.php |
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| author | D. Robakowska-Hyżorek R.R. Starzyński B. Żelazowska J. Oprządek T. Sadkowski L. Zwierzchowski |
| author_facet | D. Robakowska-Hyżorek R.R. Starzyński B. Żelazowska J. Oprządek T. Sadkowski L. Zwierzchowski |
| author_sort | D. Robakowska-Hyżorek |
| collection | DOAJ |
| description | The aim of the study was to characterize nucleotide sequence polymorphisms in the bovine PTPRQ gene, to search for their possible effect on the expression of myogenic factor 6 (MYF6; MRF4) and myogenic factor 5 (MYF5) genes in skeletal muscle and on meat production traits. Three novel SNPs were found in intron 35 of the bovine PTPRQ gene: g.200,451A>G, g.200,467T>C, g.200,480C>T (GenBank Acc. No. NW_001494990.2; counted from translation initiation site). These SNPs are placed very closely to each other (within 29 base pairs). The results showed that genotype influenced the expression of MYF6 and MYF5 genes in longissimus dorsi muscle of Limousine bulls both at the transcript and protein levels. Moreover, an association was found between the PTPRQ genotype and carcass traits in Limousine bulls. These findings suggest that bovine PTPRQ gene may contain regulatory sequences for MRF genes located 24 kb downstream. The results also showed that nucleotide sequence polymorphisms in the PTPRQ gene may influence meat production traits in beef cattle, possibly through the regulation of the MRF genes expression. |
| first_indexed | 2024-04-10T08:25:22Z |
| format | Article |
| id | doaj.art-b71c5e7510f743b4afb8dadc5251c222 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:22Z |
| publishDate | 2016-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-b71c5e7510f743b4afb8dadc5251c2222023-02-23T03:33:24ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092016-05-0161523224210.17221/118/2014-CJAScjs-201605-0005Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bullsD. Robakowska-Hyżorek0R.R. Starzyński1B. Żelazowska2J. Oprządek3T. Sadkowski4L. Zwierzchowski5Department of Neuroendocrinology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna, PolandDepartment of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, PolandDepartment of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, PolandDepartment of Animal Improvement, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, PolandDepartment of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, PolandDepartment of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, PolandThe aim of the study was to characterize nucleotide sequence polymorphisms in the bovine PTPRQ gene, to search for their possible effect on the expression of myogenic factor 6 (MYF6; MRF4) and myogenic factor 5 (MYF5) genes in skeletal muscle and on meat production traits. Three novel SNPs were found in intron 35 of the bovine PTPRQ gene: g.200,451A>G, g.200,467T>C, g.200,480C>T (GenBank Acc. No. NW_001494990.2; counted from translation initiation site). These SNPs are placed very closely to each other (within 29 base pairs). The results showed that genotype influenced the expression of MYF6 and MYF5 genes in longissimus dorsi muscle of Limousine bulls both at the transcript and protein levels. Moreover, an association was found between the PTPRQ genotype and carcass traits in Limousine bulls. These findings suggest that bovine PTPRQ gene may contain regulatory sequences for MRF genes located 24 kb downstream. The results also showed that nucleotide sequence polymorphisms in the PTPRQ gene may influence meat production traits in beef cattle, possibly through the regulation of the MRF genes expression.https://cjas.agriculturejournals.cz/artkey/cjs-201605-0005_effects-of-polymorphism-in-the-bovine-ptprq-gene-on-the-expression-of-myf6-and-myf5-genes-in-skeletal-muscle-an.phpreceptor-type iii ptp with phosphatidylinositol phosphatase activitygenotypemuscle regulatory factorsbovinemeat traits |
| spellingShingle | D. Robakowska-Hyżorek R.R. Starzyński B. Żelazowska J. Oprządek T. Sadkowski L. Zwierzchowski Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls Czech Journal of Animal Science receptor-type iii ptp with phosphatidylinositol phosphatase activity genotype muscle regulatory factors bovine meat traits |
| title | Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls |
| title_full | Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls |
| title_fullStr | Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls |
| title_full_unstemmed | Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls |
| title_short | Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls |
| title_sort | effects of polymorphism in the bovine ptprq gene on the expression of myf6 and myf5 genes in skeletal muscle and on meat production traits in beef bulls |
| topic | receptor-type iii ptp with phosphatidylinositol phosphatase activity genotype muscle regulatory factors bovine meat traits |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201605-0005_effects-of-polymorphism-in-the-bovine-ptprq-gene-on-the-expression-of-myf6-and-myf5-genes-in-skeletal-muscle-an.php |
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