Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes
Sarcopenia is a progressive muscle disease characterized by the loss of skeletal muscle mass, strength, function, and physical performance. Since the disease code was assigned, attention has been focused on natural products that can protect against muscle atrophy. <i>Cibotium barometz</i>...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/19/14798 |
| _version_ | 1797447262299750400 |
|---|---|
| author | Na-Hyung Kim Joo-Yeon Lee Choon Young Kim |
| author_facet | Na-Hyung Kim Joo-Yeon Lee Choon Young Kim |
| author_sort | Na-Hyung Kim |
| collection | DOAJ |
| description | Sarcopenia is a progressive muscle disease characterized by the loss of skeletal muscle mass, strength, function, and physical performance. Since the disease code was assigned, attention has been focused on natural products that can protect against muscle atrophy. <i>Cibotium barometz</i> (Cibotium Rhizome) has been used as an herbal medicine for the treatment of bone or joint diseases in Asian countries. However, no studies have identified the mechanism of action of Cibotium Rhizome on muscle atrophy related to sarcopenia at the site of myotubes. The aim of this study was to investigate the improvement effect of the ethanol extract of Cibotium Rhizome (ECR) on dexamethasone-induced muscle atrophy in an in vitro cell model, i.e., the C2C12 myotubes. High-performance liquid chromatography was performed to examine the phytochemicals in ECR. Seven peaks in the ECR were identified, corresponding to the following compounds: protocatechuic acid, (+)-catechin hydrate, <i>p</i>-coumaric acid, ellagic acid, chlorogenic acid, caffeic acid, and ferulic acid. In atrophy-like conditions induced by 100 μM dexamethasone for 24 h in C2C12, ECR increased the expression of the myosin heavy chain, p-Akt, the p-mammalian target of rapamycin (mTOR), p-p70S6K, and repressed the expression of regulated in development and DNA damage responses 1 (REDD1), kruppel-like factor 15 (KLF 15), muscle atrophy F-box, and muscle-specific RING finger protein-1 in C2C12. In addition, ECR alleviated dexamethasone-induced muscle atrophy by repressing REDD1 and KLF15 transcription in C2C12 myotubes, indicating the need for further studies to provide a scientific basis for the development of useful therapeutic agents using ECR to alleviate the effects of skeletal muscle atrophy or sarcopenia. |
| first_indexed | 2024-03-09T13:52:20Z |
| format | Article |
| id | doaj.art-b71ed5e9a9b048a2a75d6362e4b680ee |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:52:20Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b71ed5e9a9b048a2a75d6362e4b680ee2023-11-30T20:48:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124191479810.3390/ijms241914798Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 MyotubesNa-Hyung Kim0Joo-Yeon Lee1Choon Young Kim2Department of Food and Nutrition, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaDepartment of Food and Nutrition, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaDepartment of Food and Nutrition, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaSarcopenia is a progressive muscle disease characterized by the loss of skeletal muscle mass, strength, function, and physical performance. Since the disease code was assigned, attention has been focused on natural products that can protect against muscle atrophy. <i>Cibotium barometz</i> (Cibotium Rhizome) has been used as an herbal medicine for the treatment of bone or joint diseases in Asian countries. However, no studies have identified the mechanism of action of Cibotium Rhizome on muscle atrophy related to sarcopenia at the site of myotubes. The aim of this study was to investigate the improvement effect of the ethanol extract of Cibotium Rhizome (ECR) on dexamethasone-induced muscle atrophy in an in vitro cell model, i.e., the C2C12 myotubes. High-performance liquid chromatography was performed to examine the phytochemicals in ECR. Seven peaks in the ECR were identified, corresponding to the following compounds: protocatechuic acid, (+)-catechin hydrate, <i>p</i>-coumaric acid, ellagic acid, chlorogenic acid, caffeic acid, and ferulic acid. In atrophy-like conditions induced by 100 μM dexamethasone for 24 h in C2C12, ECR increased the expression of the myosin heavy chain, p-Akt, the p-mammalian target of rapamycin (mTOR), p-p70S6K, and repressed the expression of regulated in development and DNA damage responses 1 (REDD1), kruppel-like factor 15 (KLF 15), muscle atrophy F-box, and muscle-specific RING finger protein-1 in C2C12. In addition, ECR alleviated dexamethasone-induced muscle atrophy by repressing REDD1 and KLF15 transcription in C2C12 myotubes, indicating the need for further studies to provide a scientific basis for the development of useful therapeutic agents using ECR to alleviate the effects of skeletal muscle atrophy or sarcopenia.https://www.mdpi.com/1422-0067/24/19/14798<i>Cibotium barometz</i> (Cibotium Rhizome)sarcopeniamuscle atrophyregulated in development and DNA damage responses 1kruppel-like factor 15 |
| spellingShingle | Na-Hyung Kim Joo-Yeon Lee Choon Young Kim Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes International Journal of Molecular Sciences <i>Cibotium barometz</i> (Cibotium Rhizome) sarcopenia muscle atrophy regulated in development and DNA damage responses 1 kruppel-like factor 15 |
| title | Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes |
| title_full | Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes |
| title_fullStr | Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes |
| title_full_unstemmed | Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes |
| title_short | Protective Role of Ethanol Extract of <i>Cibotium barometz</i> (Cibotium Rhizome) against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes |
| title_sort | protective role of ethanol extract of i cibotium barometz i cibotium rhizome against dexamethasone induced muscle atrophy in c2c12 myotubes |
| topic | <i>Cibotium barometz</i> (Cibotium Rhizome) sarcopenia muscle atrophy regulated in development and DNA damage responses 1 kruppel-like factor 15 |
| url | https://www.mdpi.com/1422-0067/24/19/14798 |
| work_keys_str_mv | AT nahyungkim protectiveroleofethanolextractoficibotiumbarometzicibotiumrhizomeagainstdexamethasoneinducedmuscleatrophyinc2c12myotubes AT jooyeonlee protectiveroleofethanolextractoficibotiumbarometzicibotiumrhizomeagainstdexamethasoneinducedmuscleatrophyinc2c12myotubes AT choonyoungkim protectiveroleofethanolextractoficibotiumbarometzicibotiumrhizomeagainstdexamethasoneinducedmuscleatrophyinc2c12myotubes |