Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety
Occurrence of multi-class antibiotics in edible fish species from the Saudi market was investigated. A fast and sensitive UPLC-MS/MS method for simultaneous determination of selected fluoroquinolones, sulfonamides and macrolides in fish muscle was developed and validated. Sample clean up was perform...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Toxicology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022002232 |
| _version_ | 1797798895010447360 |
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| author | Heba Shaaban Ahmed Mostafa |
| author_facet | Heba Shaaban Ahmed Mostafa |
| author_sort | Heba Shaaban |
| collection | DOAJ |
| description | Occurrence of multi-class antibiotics in edible fish species from the Saudi market was investigated. A fast and sensitive UPLC-MS/MS method for simultaneous determination of selected fluoroquinolones, sulfonamides and macrolides in fish muscle was developed and validated. Sample clean up was performed using solid-phase extraction on Oasis HLB cartridges. The greenness profile of the developed method was evaluated using three assessment tools: analytical Eco-scale assessment method, green analytical procedure index and analytical greenness metric. Detection limits ranged from 0.008 to 0.35 μg/kg. The recovery ranged from 80.1 % to 98.6 % with RSDs ≤ 12.1 %. The mean and maximum concentrations of the detected antibiotics in fish samples ranged between 0.28 and 19.15 and 3.50–112.00 µg/kg, respectively. Human antibiotics (clarithromycin and roxithromycin) were detected in 50 % and 27.5 % of the samples, respectively. The estimated daily intake for the detected antibiotics ranged from 0.10 to 6.61 ng/kg/ BW/day for Saudi adults. The health risk associated with fish consumption was evaluated. The results suggested that fish consumption may not pose a serious risk to consumers in Saudi Arabia. The developed method was fast, sensitive, cost-effective and environmentally friendly. |
| first_indexed | 2024-03-13T04:11:01Z |
| format | Article |
| id | doaj.art-b71f20c74f994e55a92a244cf2dc1039 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-03-13T04:11:01Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-b71f20c74f994e55a92a244cf2dc10392023-06-21T06:54:58ZengElsevierToxicology Reports2214-75002023-01-0110110Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safetyHeba Shaaban0Ahmed Mostafa1Correspondence to: Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, King Faisal Road, P.O. Box 1982, Dammam, Eastern Province 31441, Saudi Arabia.; Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, King Faisal Road, P.O. Box 1982, Dammam 31441, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, King Faisal Road, P.O. Box 1982, Dammam 31441, Saudi ArabiaOccurrence of multi-class antibiotics in edible fish species from the Saudi market was investigated. A fast and sensitive UPLC-MS/MS method for simultaneous determination of selected fluoroquinolones, sulfonamides and macrolides in fish muscle was developed and validated. Sample clean up was performed using solid-phase extraction on Oasis HLB cartridges. The greenness profile of the developed method was evaluated using three assessment tools: analytical Eco-scale assessment method, green analytical procedure index and analytical greenness metric. Detection limits ranged from 0.008 to 0.35 μg/kg. The recovery ranged from 80.1 % to 98.6 % with RSDs ≤ 12.1 %. The mean and maximum concentrations of the detected antibiotics in fish samples ranged between 0.28 and 19.15 and 3.50–112.00 µg/kg, respectively. Human antibiotics (clarithromycin and roxithromycin) were detected in 50 % and 27.5 % of the samples, respectively. The estimated daily intake for the detected antibiotics ranged from 0.10 to 6.61 ng/kg/ BW/day for Saudi adults. The health risk associated with fish consumption was evaluated. The results suggested that fish consumption may not pose a serious risk to consumers in Saudi Arabia. The developed method was fast, sensitive, cost-effective and environmentally friendly.http://www.sciencedirect.com/science/article/pii/S2214750022002232UPLC-MS/MSAquacultureAntibioticsGreenness assessmentHuman health riskEstimated daily intake |
| spellingShingle | Heba Shaaban Ahmed Mostafa Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety Toxicology Reports UPLC-MS/MS Aquaculture Antibiotics Greenness assessment Human health risk Estimated daily intake |
| title | Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety |
| title_full | Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety |
| title_fullStr | Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety |
| title_full_unstemmed | Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety |
| title_short | Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety |
| title_sort | simultaneous determination of antibiotics residues in edible fish muscle using eco friendly spe uplc ms ms occurrence human dietary exposure and health risk assessment for consumer safety |
| topic | UPLC-MS/MS Aquaculture Antibiotics Greenness assessment Human health risk Estimated daily intake |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022002232 |
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