Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D

Through the formation of fluorescent self-interference (SELFI), quantitative intensity and phase imaging enables the 3D localization of single fluorescent molecules inside a fixed tissue with an accuracy well-beyond the diffraction limit. Here we demonstrate that this concept can be extended to 3D s...

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Main Authors: Jeanne Linarès-Loyez, Joana S. Ferreira, Olivier Rossier, Brahim Lounis, Gregory Giannone, Laurent Groc, Laurent Cognet, Pierre Bon
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-05-01
Series:Frontiers in Physics
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fphy.2019.00068/full
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author Jeanne Linarès-Loyez
Jeanne Linarès-Loyez
Joana S. Ferreira
Joana S. Ferreira
Olivier Rossier
Olivier Rossier
Brahim Lounis
Brahim Lounis
Gregory Giannone
Gregory Giannone
Laurent Groc
Laurent Groc
Laurent Cognet
Laurent Cognet
Pierre Bon
Pierre Bon
author_facet Jeanne Linarès-Loyez
Jeanne Linarès-Loyez
Joana S. Ferreira
Joana S. Ferreira
Olivier Rossier
Olivier Rossier
Brahim Lounis
Brahim Lounis
Gregory Giannone
Gregory Giannone
Laurent Groc
Laurent Groc
Laurent Cognet
Laurent Cognet
Pierre Bon
Pierre Bon
author_sort Jeanne Linarès-Loyez
collection DOAJ
description Through the formation of fluorescent self-interference (SELFI), quantitative intensity and phase imaging enables the 3D localization of single fluorescent molecules inside a fixed tissue with an accuracy well-beyond the diffraction limit. Here we demonstrate that this concept can be extended to 3D super-resolution microscopy and 3D single particle tracking in various living samples ranging from adherent cells to organotypic brain slices, using diverse fluorescent emitters (fluorescent proteins, organic dyes or quantum dots). This basically covers the most popular single molecule imaging techniques used for live sample studies. We also show that SELFI can be used in combination with different illumination schemes including highly inclined illumination and total internal reflection.
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spelling doaj.art-b7205b0f9bea4a7a802ab84258568da42022-12-22T00:34:03ZengFrontiers Media S.A.Frontiers in Physics2296-424X2019-05-01710.3389/fphy.2019.00068450794Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3DJeanne Linarès-Loyez0Jeanne Linarès-Loyez1Joana S. Ferreira2Joana S. Ferreira3Olivier Rossier4Olivier Rossier5Brahim Lounis6Brahim Lounis7Gregory Giannone8Gregory Giannone9Laurent Groc10Laurent Groc11Laurent Cognet12Laurent Cognet13Pierre Bon14Pierre Bon15Laboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceLaboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceLaboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceLaboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceThrough the formation of fluorescent self-interference (SELFI), quantitative intensity and phase imaging enables the 3D localization of single fluorescent molecules inside a fixed tissue with an accuracy well-beyond the diffraction limit. Here we demonstrate that this concept can be extended to 3D super-resolution microscopy and 3D single particle tracking in various living samples ranging from adherent cells to organotypic brain slices, using diverse fluorescent emitters (fluorescent proteins, organic dyes or quantum dots). This basically covers the most popular single molecule imaging techniques used for live sample studies. We also show that SELFI can be used in combination with different illumination schemes including highly inclined illumination and total internal reflection.https://www.frontiersin.org/article/10.3389/fphy.2019.00068/fullsuper-resolution (SR) imagingsingle molecule localization microscopy (SMLM)phase imaginginterferometryfluorescencesingle particle tracking (SPT)
spellingShingle Jeanne Linarès-Loyez
Jeanne Linarès-Loyez
Joana S. Ferreira
Joana S. Ferreira
Olivier Rossier
Olivier Rossier
Brahim Lounis
Brahim Lounis
Gregory Giannone
Gregory Giannone
Laurent Groc
Laurent Groc
Laurent Cognet
Laurent Cognet
Pierre Bon
Pierre Bon
Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
Frontiers in Physics
super-resolution (SR) imaging
single molecule localization microscopy (SMLM)
phase imaging
interferometry
fluorescence
single particle tracking (SPT)
title Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
title_full Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
title_fullStr Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
title_full_unstemmed Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
title_short Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
title_sort self interference selfi microscopy for live super resolution imaging and single particle tracking in 3d
topic super-resolution (SR) imaging
single molecule localization microscopy (SMLM)
phase imaging
interferometry
fluorescence
single particle tracking (SPT)
url https://www.frontiersin.org/article/10.3389/fphy.2019.00068/full
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