Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
Through the formation of fluorescent self-interference (SELFI), quantitative intensity and phase imaging enables the 3D localization of single fluorescent molecules inside a fixed tissue with an accuracy well-beyond the diffraction limit. Here we demonstrate that this concept can be extended to 3D s...
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Frontiers Media S.A.
2019-05-01
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Series: | Frontiers in Physics |
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Online Access: | https://www.frontiersin.org/article/10.3389/fphy.2019.00068/full |
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author | Jeanne Linarès-Loyez Jeanne Linarès-Loyez Joana S. Ferreira Joana S. Ferreira Olivier Rossier Olivier Rossier Brahim Lounis Brahim Lounis Gregory Giannone Gregory Giannone Laurent Groc Laurent Groc Laurent Cognet Laurent Cognet Pierre Bon Pierre Bon |
author_facet | Jeanne Linarès-Loyez Jeanne Linarès-Loyez Joana S. Ferreira Joana S. Ferreira Olivier Rossier Olivier Rossier Brahim Lounis Brahim Lounis Gregory Giannone Gregory Giannone Laurent Groc Laurent Groc Laurent Cognet Laurent Cognet Pierre Bon Pierre Bon |
author_sort | Jeanne Linarès-Loyez |
collection | DOAJ |
description | Through the formation of fluorescent self-interference (SELFI), quantitative intensity and phase imaging enables the 3D localization of single fluorescent molecules inside a fixed tissue with an accuracy well-beyond the diffraction limit. Here we demonstrate that this concept can be extended to 3D super-resolution microscopy and 3D single particle tracking in various living samples ranging from adherent cells to organotypic brain slices, using diverse fluorescent emitters (fluorescent proteins, organic dyes or quantum dots). This basically covers the most popular single molecule imaging techniques used for live sample studies. We also show that SELFI can be used in combination with different illumination schemes including highly inclined illumination and total internal reflection. |
first_indexed | 2024-12-12T06:51:28Z |
format | Article |
id | doaj.art-b7205b0f9bea4a7a802ab84258568da4 |
institution | Directory Open Access Journal |
issn | 2296-424X |
language | English |
last_indexed | 2024-12-12T06:51:28Z |
publishDate | 2019-05-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Physics |
spelling | doaj.art-b7205b0f9bea4a7a802ab84258568da42022-12-22T00:34:03ZengFrontiers Media S.A.Frontiers in Physics2296-424X2019-05-01710.3389/fphy.2019.00068450794Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3DJeanne Linarès-Loyez0Jeanne Linarès-Loyez1Joana S. Ferreira2Joana S. Ferreira3Olivier Rossier4Olivier Rossier5Brahim Lounis6Brahim Lounis7Gregory Giannone8Gregory Giannone9Laurent Groc10Laurent Groc11Laurent Cognet12Laurent Cognet13Pierre Bon14Pierre Bon15Laboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceLaboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceInterdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, FranceCNRS, IINS UMR 5297, Bordeaux, FranceLaboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceLaboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, FranceInstitut d'Optique & CNRS, LP2N UMR 5298, Talence, FranceThrough the formation of fluorescent self-interference (SELFI), quantitative intensity and phase imaging enables the 3D localization of single fluorescent molecules inside a fixed tissue with an accuracy well-beyond the diffraction limit. Here we demonstrate that this concept can be extended to 3D super-resolution microscopy and 3D single particle tracking in various living samples ranging from adherent cells to organotypic brain slices, using diverse fluorescent emitters (fluorescent proteins, organic dyes or quantum dots). This basically covers the most popular single molecule imaging techniques used for live sample studies. We also show that SELFI can be used in combination with different illumination schemes including highly inclined illumination and total internal reflection.https://www.frontiersin.org/article/10.3389/fphy.2019.00068/fullsuper-resolution (SR) imagingsingle molecule localization microscopy (SMLM)phase imaginginterferometryfluorescencesingle particle tracking (SPT) |
spellingShingle | Jeanne Linarès-Loyez Jeanne Linarès-Loyez Joana S. Ferreira Joana S. Ferreira Olivier Rossier Olivier Rossier Brahim Lounis Brahim Lounis Gregory Giannone Gregory Giannone Laurent Groc Laurent Groc Laurent Cognet Laurent Cognet Pierre Bon Pierre Bon Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D Frontiers in Physics super-resolution (SR) imaging single molecule localization microscopy (SMLM) phase imaging interferometry fluorescence single particle tracking (SPT) |
title | Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D |
title_full | Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D |
title_fullStr | Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D |
title_full_unstemmed | Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D |
title_short | Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D |
title_sort | self interference selfi microscopy for live super resolution imaging and single particle tracking in 3d |
topic | super-resolution (SR) imaging single molecule localization microscopy (SMLM) phase imaging interferometry fluorescence single particle tracking (SPT) |
url | https://www.frontiersin.org/article/10.3389/fphy.2019.00068/full |
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