<em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seeds
The availability of D. strictus seeds is affected by the length and erratic flowering cycle of the species. These seeds also retain their germination only for short periods of time. The tissue culture offers a fast and reliable way for the propagation using in vitro seed germinated as initial plant...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2007-01-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/355 |
| _version_ | 1828400426131652608 |
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| author | Yudith García-Ramírez Marisol Freire-Seijo Marisol Tejeda Maritza Reyes |
| author_facet | Yudith García-Ramírez Marisol Freire-Seijo Marisol Tejeda Maritza Reyes |
| author_sort | Yudith García-Ramírez |
| collection | DOAJ |
| description | The availability of D. strictus seeds is affected by the length and erratic flowering cycle of the species. These seeds also retain their germination only for short periods of time. The tissue culture offers a fast and reliable way for the propagation using in vitro seed germinated as initial plant material. This research was conducted with the objective of to germinate in vitro D. strictus seeds. The effect of different concentrations of sodium hypochlorite (NaClO) (3.0, 5.0 and 7.0%) and time for seed disinfection (20 and 30 min) were determined. It was evaluated the number of germinated seeds and free of microbial contaminants after 10 days of culture. The results showed that the method of disinfection which sodium hypochlorite 3.0% for 30 minutes was the best for in vitro germination of Dendrocalamus strictus (Rosb.) Nees seeds.
Key words: bamboos, disinfection, sodium hypochlorite, tissue culture |
| first_indexed | 2024-12-10T09:31:20Z |
| format | Article |
| id | doaj.art-b724e097c86d4fc2b24e3477d30bf738 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-10T09:31:20Z |
| publishDate | 2007-01-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-b724e097c86d4fc2b24e3477d30bf7382022-12-22T01:54:20ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472007-01-0171328<em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seedsYudith García-RamírezMarisol Freire-SeijoMarisol TejedaMaritza ReyesThe availability of D. strictus seeds is affected by the length and erratic flowering cycle of the species. These seeds also retain their germination only for short periods of time. The tissue culture offers a fast and reliable way for the propagation using in vitro seed germinated as initial plant material. This research was conducted with the objective of to germinate in vitro D. strictus seeds. The effect of different concentrations of sodium hypochlorite (NaClO) (3.0, 5.0 and 7.0%) and time for seed disinfection (20 and 30 min) were determined. It was evaluated the number of germinated seeds and free of microbial contaminants after 10 days of culture. The results showed that the method of disinfection which sodium hypochlorite 3.0% for 30 minutes was the best for in vitro germination of Dendrocalamus strictus (Rosb.) Nees seeds. Key words: bamboos, disinfection, sodium hypochlorite, tissue culturehttps://revista.ibp.co.cu/index.php/BV/article/view/355 |
| spellingShingle | Yudith García-Ramírez Marisol Freire-Seijo Marisol Tejeda Maritza Reyes <em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seeds Biotecnología Vegetal |
| title | <em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seeds |
| title_full | <em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seeds |
| title_fullStr | <em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seeds |
| title_full_unstemmed | <em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seeds |
| title_short | <em>In vitro </em>germination of <em>Dendrocalamus strictus </em>(Rosb.) Nees seeds |
| title_sort | em in vitro em germination of em dendrocalamus strictus em rosb nees seeds |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/355 |
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