Ameliorative effects of Lactobacillus against Aflatoxin B1
Abstract Lactobacilli are probiotics with Aflatoxin (AF) detoxification ability, found in fermented products, GIT of animals and environment. Purpose of this study was to investigate the ability of broiler isolates of Lactobacillus against Aflatoxin B1 (AFB1). For this purpose, 5 isolates of Lactoba...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-12-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100143&tlng=en |
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| author | R. Yasmeen B. Zahid S. Alyas R. Akhtar N. Zahra S. Kouser A. S. Hashmi M. Athar M. Tayyab A. A. Anjum |
| author_facet | R. Yasmeen B. Zahid S. Alyas R. Akhtar N. Zahra S. Kouser A. S. Hashmi M. Athar M. Tayyab A. A. Anjum |
| author_sort | R. Yasmeen |
| collection | DOAJ |
| description | Abstract Lactobacilli are probiotics with Aflatoxin (AF) detoxification ability, found in fermented products, GIT of animals and environment. Purpose of this study was to investigate the ability of broiler isolates of Lactobacillus against Aflatoxin B1 (AFB1). For this purpose, 5 isolates of Lactobacillus from broiler gut were incubated with 100 ppb AFB1 in aqueous environment and effect of different parameters (cell fractions, time, temperature, pH) on detoxification was determined by HPLC. The ameliorative effect of Lactobacillus salivarius (LS) against AFB1 was studied in broiler. The results revealed that LS (CR. 4) showed the best results (in vitro) as compared to other isolates (L. salivarius (CR. 3, CR, 4), L. agilis (CE. 2.1, CE. 3.1) and L. crispatus (CE. 28). Cell debris of CR. 4 showed significantly higher detoxification (P<0.05). Maximum amount of AFB1 was detoxified at 30°C (97%), pH 4.0 (99%) and 6 h (99.97%). In vivo study showed that AFB1 decreased weight gain (1,269 ± 0.04 gm/ bird), feed consumed (2,161 ± 0.08 gm/ bird), serum total protein (2.42 ± 0.34 gm/ dl), serum albumin (0.5 ± 0.2 2 gm/dl) and antibody titer (4.2 ± 0.83). Liver function enzymes were found (alanine transaminase (ALT): 32 ± 10.7 U/L) and aspartate transaminase (AST): 314.8 ± 27 U/L) elevated in AFB1 fed broilers. Treatment with 1% LS not only decreased the toxic effects of AFB1 (group D) but also improved the overall health of broilers due to its probiotic effects (p<0.05) as compared to control negative (group A). The detoxification ability of LS was better than commercial binder (CB) (0.2% Protmyc). It was concluded that detoxification of AFB1 by Lactobacillus was strain, temperature, pH and time dependent. LS has detoxification ability against AFB1 in vivo. |
| first_indexed | 2024-12-20T09:42:41Z |
| format | Article |
| id | doaj.art-b72661bda3184ec1afff6491b7e3d5ab |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-20T09:42:41Z |
| publishDate | 2021-12-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-b72661bda3184ec1afff6491b7e3d5ab2022-12-21T19:44:51ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-12-018410.1590/1519-6984.250517Ameliorative effects of Lactobacillus against Aflatoxin B1R. Yasmeenhttps://orcid.org/0000-0003-3911-3558B. Zahidhttps://orcid.org/0000-0003-2050-9173S. Alyashttps://orcid.org/0000-0002-9971-8121R. Akhtarhttps://orcid.org/0000-0003-0501-3989N. Zahrahttps://orcid.org/0000-0001-8902-8509S. Kouserhttps://orcid.org/0000-0002-8674-1234A. S. Hashmihttps://orcid.org/0000-0001-5368-888XM. Atharhttps://orcid.org/0000-0002-8693-1409M. Tayyabhttps://orcid.org/0000-0001-8192-9169A. A. Anjumhttps://orcid.org/0000-0001-8262-8720Abstract Lactobacilli are probiotics with Aflatoxin (AF) detoxification ability, found in fermented products, GIT of animals and environment. Purpose of this study was to investigate the ability of broiler isolates of Lactobacillus against Aflatoxin B1 (AFB1). For this purpose, 5 isolates of Lactobacillus from broiler gut were incubated with 100 ppb AFB1 in aqueous environment and effect of different parameters (cell fractions, time, temperature, pH) on detoxification was determined by HPLC. The ameliorative effect of Lactobacillus salivarius (LS) against AFB1 was studied in broiler. The results revealed that LS (CR. 4) showed the best results (in vitro) as compared to other isolates (L. salivarius (CR. 3, CR, 4), L. agilis (CE. 2.1, CE. 3.1) and L. crispatus (CE. 28). Cell debris of CR. 4 showed significantly higher detoxification (P<0.05). Maximum amount of AFB1 was detoxified at 30°C (97%), pH 4.0 (99%) and 6 h (99.97%). In vivo study showed that AFB1 decreased weight gain (1,269 ± 0.04 gm/ bird), feed consumed (2,161 ± 0.08 gm/ bird), serum total protein (2.42 ± 0.34 gm/ dl), serum albumin (0.5 ± 0.2 2 gm/dl) and antibody titer (4.2 ± 0.83). Liver function enzymes were found (alanine transaminase (ALT): 32 ± 10.7 U/L) and aspartate transaminase (AST): 314.8 ± 27 U/L) elevated in AFB1 fed broilers. Treatment with 1% LS not only decreased the toxic effects of AFB1 (group D) but also improved the overall health of broilers due to its probiotic effects (p<0.05) as compared to control negative (group A). The detoxification ability of LS was better than commercial binder (CB) (0.2% Protmyc). It was concluded that detoxification of AFB1 by Lactobacillus was strain, temperature, pH and time dependent. LS has detoxification ability against AFB1 in vivo.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100143&tlng=enL. salivariusAflatoxin B1detoxificationHPLC |
| spellingShingle | R. Yasmeen B. Zahid S. Alyas R. Akhtar N. Zahra S. Kouser A. S. Hashmi M. Athar M. Tayyab A. A. Anjum Ameliorative effects of Lactobacillus against Aflatoxin B1 Brazilian Journal of Biology L. salivarius Aflatoxin B1 detoxification HPLC |
| title | Ameliorative effects of Lactobacillus against Aflatoxin B1 |
| title_full | Ameliorative effects of Lactobacillus against Aflatoxin B1 |
| title_fullStr | Ameliorative effects of Lactobacillus against Aflatoxin B1 |
| title_full_unstemmed | Ameliorative effects of Lactobacillus against Aflatoxin B1 |
| title_short | Ameliorative effects of Lactobacillus against Aflatoxin B1 |
| title_sort | ameliorative effects of lactobacillus against aflatoxin b1 |
| topic | L. salivarius Aflatoxin B1 detoxification HPLC |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100143&tlng=en |
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