Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit

Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawbe...

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Bibliographic Details
Main Authors: Wenjing Lu, Xiaopeng Wei, Xueyuan Han, Renchi Chen, Chaogeng Xiao, Xiaojie Zheng, Linchun Mao
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/12/9/1802
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Summary:Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco rattle virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related <i>PAL6</i>, <i>C4H</i>, <i>4CL</i>, <i>DFR</i>, and <i>UFGT</i>, softening-related <i>PL</i> and <i>XTH</i>, and aroma-related <i>QR</i> and <i>AAT2.</i> In addition, the interaction between <i>FaMADS1</i> and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the <i>FaMADS1</i> promoter could interact with FaABI5-5, FaTRAB1, and FaABI5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the <i>FaMADS1</i> promoter, resulting in the decreased expression of <i>FaMADS1</i>. In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting <i>FaMADS1</i> expression by the active binding of transcript FaTRAB1 with the <i>FaMADS1</i> promoter.
ISSN:2304-8158