Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit
Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawbe...
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MDPI AG
2023-04-01
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author | Wenjing Lu Xiaopeng Wei Xueyuan Han Renchi Chen Chaogeng Xiao Xiaojie Zheng Linchun Mao |
author_facet | Wenjing Lu Xiaopeng Wei Xueyuan Han Renchi Chen Chaogeng Xiao Xiaojie Zheng Linchun Mao |
author_sort | Wenjing Lu |
collection | DOAJ |
description | Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco rattle virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related <i>PAL6</i>, <i>C4H</i>, <i>4CL</i>, <i>DFR</i>, and <i>UFGT</i>, softening-related <i>PL</i> and <i>XTH</i>, and aroma-related <i>QR</i> and <i>AAT2.</i> In addition, the interaction between <i>FaMADS1</i> and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the <i>FaMADS1</i> promoter could interact with FaABI5-5, FaTRAB1, and FaABI5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the <i>FaMADS1</i> promoter, resulting in the decreased expression of <i>FaMADS1</i>. In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting <i>FaMADS1</i> expression by the active binding of transcript FaTRAB1 with the <i>FaMADS1</i> promoter. |
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spelling | doaj.art-b7279c8116024299988a6ef1f39870212023-11-17T22:55:04ZengMDPI AGFoods2304-81582023-04-01129180210.3390/foods12091802Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry FruitWenjing Lu0Xiaopeng Wei1Xueyuan Han2Renchi Chen3Chaogeng Xiao4Xiaojie Zheng5Linchun Mao6Institute of Food Science, Zhejiang Academy of Agricultural Sciences, 298 Desheng Road, Hangzhou 310021, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, 298 Desheng Road, Hangzhou 310021, ChinaDepartment of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaAbscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco rattle virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related <i>PAL6</i>, <i>C4H</i>, <i>4CL</i>, <i>DFR</i>, and <i>UFGT</i>, softening-related <i>PL</i> and <i>XTH</i>, and aroma-related <i>QR</i> and <i>AAT2.</i> In addition, the interaction between <i>FaMADS1</i> and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the <i>FaMADS1</i> promoter could interact with FaABI5-5, FaTRAB1, and FaABI5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the <i>FaMADS1</i> promoter, resulting in the decreased expression of <i>FaMADS1</i>. In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting <i>FaMADS1</i> expression by the active binding of transcript FaTRAB1 with the <i>FaMADS1</i> promoter.https://www.mdpi.com/2304-8158/12/9/1802phytohormone<i>Fragaria × ananassa</i>fruit ripeningMADSTRAB |
spellingShingle | Wenjing Lu Xiaopeng Wei Xueyuan Han Renchi Chen Chaogeng Xiao Xiaojie Zheng Linchun Mao Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit Foods phytohormone <i>Fragaria × ananassa</i> fruit ripening MADS TRAB |
title | Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit |
title_full | Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit |
title_fullStr | Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit |
title_full_unstemmed | Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit |
title_short | Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit |
title_sort | participation of fatrab1 transcription factor in the regulation of i famads1 i involved in aba dependent ripening of strawberry fruit |
topic | phytohormone <i>Fragaria × ananassa</i> fruit ripening MADS TRAB |
url | https://www.mdpi.com/2304-8158/12/9/1802 |
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