Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit

Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawbe...

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Main Authors: Wenjing Lu, Xiaopeng Wei, Xueyuan Han, Renchi Chen, Chaogeng Xiao, Xiaojie Zheng, Linchun Mao
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/12/9/1802
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author Wenjing Lu
Xiaopeng Wei
Xueyuan Han
Renchi Chen
Chaogeng Xiao
Xiaojie Zheng
Linchun Mao
author_facet Wenjing Lu
Xiaopeng Wei
Xueyuan Han
Renchi Chen
Chaogeng Xiao
Xiaojie Zheng
Linchun Mao
author_sort Wenjing Lu
collection DOAJ
description Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco rattle virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related <i>PAL6</i>, <i>C4H</i>, <i>4CL</i>, <i>DFR</i>, and <i>UFGT</i>, softening-related <i>PL</i> and <i>XTH</i>, and aroma-related <i>QR</i> and <i>AAT2.</i> In addition, the interaction between <i>FaMADS1</i> and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the <i>FaMADS1</i> promoter could interact with FaABI5-5, FaTRAB1, and FaABI5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the <i>FaMADS1</i> promoter, resulting in the decreased expression of <i>FaMADS1</i>. In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting <i>FaMADS1</i> expression by the active binding of transcript FaTRAB1 with the <i>FaMADS1</i> promoter.
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spelling doaj.art-b7279c8116024299988a6ef1f39870212023-11-17T22:55:04ZengMDPI AGFoods2304-81582023-04-01129180210.3390/foods12091802Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry FruitWenjing Lu0Xiaopeng Wei1Xueyuan Han2Renchi Chen3Chaogeng Xiao4Xiaojie Zheng5Linchun Mao6Institute of Food Science, Zhejiang Academy of Agricultural Sciences, 298 Desheng Road, Hangzhou 310021, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, 298 Desheng Road, Hangzhou 310021, ChinaDepartment of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, ChinaZhejiang Key Laboratory of AgroFood Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaAbscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteric strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of <i>FaMADS1</i>. The transient silence of <i>FaMADS1</i> in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco rattle virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related <i>PAL6</i>, <i>C4H</i>, <i>4CL</i>, <i>DFR</i>, and <i>UFGT</i>, softening-related <i>PL</i> and <i>XTH</i>, and aroma-related <i>QR</i> and <i>AAT2.</i> In addition, the interaction between <i>FaMADS1</i> and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the <i>FaMADS1</i> promoter could interact with FaABI5-5, FaTRAB1, and FaABI5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the <i>FaMADS1</i> promoter, resulting in the decreased expression of <i>FaMADS1</i>. In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting <i>FaMADS1</i> expression by the active binding of transcript FaTRAB1 with the <i>FaMADS1</i> promoter.https://www.mdpi.com/2304-8158/12/9/1802phytohormone<i>Fragaria × ananassa</i>fruit ripeningMADSTRAB
spellingShingle Wenjing Lu
Xiaopeng Wei
Xueyuan Han
Renchi Chen
Chaogeng Xiao
Xiaojie Zheng
Linchun Mao
Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit
Foods
phytohormone
<i>Fragaria × ananassa</i>
fruit ripening
MADS
TRAB
title Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit
title_full Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit
title_fullStr Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit
title_full_unstemmed Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit
title_short Participation of FaTRAB1 Transcription Factor in the Regulation of <i>FaMADS1</i> Involved in ABA-Dependent Ripening of Strawberry Fruit
title_sort participation of fatrab1 transcription factor in the regulation of i famads1 i involved in aba dependent ripening of strawberry fruit
topic phytohormone
<i>Fragaria × ananassa</i>
fruit ripening
MADS
TRAB
url https://www.mdpi.com/2304-8158/12/9/1802
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