Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients
A major challenge in hepatitis C research is the detection of early potential for progressive liver disease. MicroRNAs (miRNAs) are small RNAs that regulate gene expression and can be biomarkers of pathological processes. In this study, we compared circulating miRNAs identified in hepatitis C virus...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580820301242 |
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| author | B.C.A. Cabral L. Hoffmann T. Bottaro P.F. Costa A.L.A. Ramos H.S.M. Coelho C.A. Villela-Nogueira T.P. Ürményi D.S. Faffe R. Silva |
| author_facet | B.C.A. Cabral L. Hoffmann T. Bottaro P.F. Costa A.L.A. Ramos H.S.M. Coelho C.A. Villela-Nogueira T.P. Ürményi D.S. Faffe R. Silva |
| author_sort | B.C.A. Cabral |
| collection | DOAJ |
| description | A major challenge in hepatitis C research is the detection of early potential for progressive liver disease. MicroRNAs (miRNAs) are small RNAs that regulate gene expression and can be biomarkers of pathological processes. In this study, we compared circulating miRNAs identified in hepatitis C virus (HCV)-infected patients presenting two extremes of liver disease: mild/moderate fibrosis and cirrhosis. The patients in the cirrhosis group subsequently developed hepatocellular carcinoma (HCC). We identified 163 mature miRNAs in the mild/moderate fibrosis group and 171 in the cirrhosis group, with 144 in common to both groups. Differential expression analysis revealed 5 upregulated miRNAs and 2 downregulated miRNAs in the cirrhosis group relative to the mild/moderate fibrosis group. Functional analyses of regulatory networks (target gene and miRNA) identified gene categories involved in cell cycle biological processes and metabolic pathways related to cell cycle, cancer, and apoptosis. These results suggest that the differentially expressed circulating miRNAs observed in this work (miR-215-5p, miR-483-5p, miR-193b-3p, miR-34a-5p, miR-885-5p, miR-26b-5p and miR -197-3p) may be candidates for biomarkers in the prognosis of liver disease. |
| first_indexed | 2024-12-17T00:07:18Z |
| format | Article |
| id | doaj.art-b7282b31e5824fd7975402b5a7cb4741 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-17T00:07:18Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-b7282b31e5824fd7975402b5a7cb47412022-12-21T22:10:55ZengElsevierBiochemistry and Biophysics Reports2405-58082020-12-0124100814Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patientsB.C.A. Cabral0L. Hoffmann1T. Bottaro2P.F. Costa3A.L.A. Ramos4H.S.M. Coelho5C.A. Villela-Nogueira6T.P. Ürményi7D.S. Faffe8R. Silva9Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Departamento de Biotecnologia, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Clínica Médica, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Clínica Médica, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Clínica Médica, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Corresponding author.A major challenge in hepatitis C research is the detection of early potential for progressive liver disease. MicroRNAs (miRNAs) are small RNAs that regulate gene expression and can be biomarkers of pathological processes. In this study, we compared circulating miRNAs identified in hepatitis C virus (HCV)-infected patients presenting two extremes of liver disease: mild/moderate fibrosis and cirrhosis. The patients in the cirrhosis group subsequently developed hepatocellular carcinoma (HCC). We identified 163 mature miRNAs in the mild/moderate fibrosis group and 171 in the cirrhosis group, with 144 in common to both groups. Differential expression analysis revealed 5 upregulated miRNAs and 2 downregulated miRNAs in the cirrhosis group relative to the mild/moderate fibrosis group. Functional analyses of regulatory networks (target gene and miRNA) identified gene categories involved in cell cycle biological processes and metabolic pathways related to cell cycle, cancer, and apoptosis. These results suggest that the differentially expressed circulating miRNAs observed in this work (miR-215-5p, miR-483-5p, miR-193b-3p, miR-34a-5p, miR-885-5p, miR-26b-5p and miR -197-3p) may be candidates for biomarkers in the prognosis of liver disease.http://www.sciencedirect.com/science/article/pii/S2405580820301242mirRNAomeCirrhosisBiomarkersHCVMassively parallel sequencing |
| spellingShingle | B.C.A. Cabral L. Hoffmann T. Bottaro P.F. Costa A.L.A. Ramos H.S.M. Coelho C.A. Villela-Nogueira T.P. Ürményi D.S. Faffe R. Silva Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients Biochemistry and Biophysics Reports mirRNAome Cirrhosis Biomarkers HCV Massively parallel sequencing |
| title | Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients |
| title_full | Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients |
| title_fullStr | Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients |
| title_full_unstemmed | Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients |
| title_short | Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients |
| title_sort | circulating micrornas associated with liver fibrosis in chronic hepatitis c patients |
| topic | mirRNAome Cirrhosis Biomarkers HCV Massively parallel sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2405580820301242 |
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