Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndrome
Objective To explore the relationship between MSX1 gene detection and tooth loss in a Van der Woude syndrome (VWS) family. Methods DNA was extracted from the venous blood of 2 patients with dental hy⁃ podontia in the 9th family of Van der Woude syndrome (VWS) families and 62 controls with complete...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2020-01-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2020.01.008 |
| _version_ | 1819145884823191552 |
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| author | DU Xinya LI Xiaoyu XIE Chun WU Bin SONG Guangbao DU Ye |
| author_facet | DU Xinya LI Xiaoyu XIE Chun WU Bin SONG Guangbao DU Ye |
| author_sort | DU Xinya |
| collection | DOAJ |
| description | Objective To explore the relationship between MSX1 gene detection and tooth loss in a Van der
Woude syndrome (VWS) family. Methods DNA was extracted from the venous blood of 2 patients with dental hy⁃
podontia in the 9th family of Van der Woude syndrome (VWS) families and 62 controls with complete dentition. Primers
were designed for the MSXl gene. The coding regions of exons 1 and 2 of the MSX1 gene were amplified by PCR. The
purified products of exons 1 and 2 of the MSX1 gene were sequenced and analyzed by sequence alignment. Results
The ivs2+68 C>T polymorphism in the MSX1 gene was found in the VWS9 members with tooth loss, and the VWS pa⁃
tients with IRF6 gene mutations had increased tooth loss. Conclusion Congenital tooth loss in the patients with con⁃
genital missing teeth in VWS family 9 may be related to the ivs2 + 68 C> T polymorphism of the MSX1 gene. |
| first_indexed | 2024-12-22T13:05:08Z |
| format | Article |
| id | doaj.art-b72d40c74f84424dafa873385dc02d5e |
| institution | Directory Open Access Journal |
| issn | 2096-1456 2096-1456 |
| language | zho |
| last_indexed | 2024-12-22T13:05:08Z |
| publishDate | 2020-01-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-b72d40c74f84424dafa873385dc02d5e2022-12-21T18:24:54ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562096-14562020-01-01281475110.12016/j.issn.2096⁃1456.2020.01.008Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndromeDU Xinya 0 LI Xiaoyu1 XIE Chun2WU Bin3 SONG Guangbao 4 DU Ye5 Department of Stomatology, the People′s Hos⁃ pital of Longhua Department of Stomatology, the People′s Hos⁃ pital of Longhua Department of Stomatology, the People′s Hos⁃ pital of Longhua Department of Stomatology, the People′s Hos⁃ pital of Longhua Department for VIP, Stomatological Hospital, Southern Medical Univer⁃ sity Department of Stomatology, the People′s Hos⁃ pital of LonghuaObjective To explore the relationship between MSX1 gene detection and tooth loss in a Van der Woude syndrome (VWS) family. Methods DNA was extracted from the venous blood of 2 patients with dental hy⁃ podontia in the 9th family of Van der Woude syndrome (VWS) families and 62 controls with complete dentition. Primers were designed for the MSXl gene. The coding regions of exons 1 and 2 of the MSX1 gene were amplified by PCR. The purified products of exons 1 and 2 of the MSX1 gene were sequenced and analyzed by sequence alignment. Results The ivs2+68 C>T polymorphism in the MSX1 gene was found in the VWS9 members with tooth loss, and the VWS pa⁃ tients with IRF6 gene mutations had increased tooth loss. Conclusion Congenital tooth loss in the patients with con⁃ genital missing teeth in VWS family 9 may be related to the ivs2 + 68 C> T polymorphism of the MSX1 gene.http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2020.01.008van der woude syndrome，congenital missing teeth，lower lip fistula，cleft lip，cleft palate，msx1，pathogenic gene，gene polymorphism，genetic mutations， |
| spellingShingle | DU Xinya LI Xiaoyu XIE Chun WU Bin SONG Guangbao DU Ye Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndrome 口腔疾病防治 van der woude syndrome， congenital missing teeth， lower lip fistula， cleft lip， cleft palate， msx1， pathogenic gene， gene polymorphism， genetic mutations， |
| title | Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndrome |
| title_full | Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndrome |
| title_fullStr | Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndrome |
| title_full_unstemmed | Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndrome |
| title_short | Detection of MSX1 gene mutations in patients with congenital tooth loss in Van der Woude syndrome |
| title_sort | detection of msx1 gene mutations in patients with congenital tooth loss in van der woude syndrome |
| topic | van der woude syndrome， congenital missing teeth， lower lip fistula， cleft lip， cleft palate， msx1， pathogenic gene， gene polymorphism， genetic mutations， |
| url | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2020.01.008 |
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