Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis.
Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain re...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3440434?pdf=render |
| _version_ | 1819103974173704192 |
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| author | Claude Nogues Hervé Leh Joseph Lautru Olivier Delelis Malcolm Buckle |
| author_facet | Claude Nogues Hervé Leh Joseph Lautru Olivier Delelis Malcolm Buckle |
| author_sort | Claude Nogues |
| collection | DOAJ |
| description | Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated. |
| first_indexed | 2024-12-22T01:58:59Z |
| format | Article |
| id | doaj.art-b72d5d13c22c435aae6484bc149c8035 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T01:58:59Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b72d5d13c22c435aae6484bc149c80352022-12-21T18:42:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4428710.1371/journal.pone.0044287Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis.Claude NoguesHervé LehJoseph LautruOlivier DelelisMalcolm BuckleNon-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.http://europepmc.org/articles/PMC3440434?pdf=render |
| spellingShingle | Claude Nogues Hervé Leh Joseph Lautru Olivier Delelis Malcolm Buckle Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis. PLoS ONE |
| title | Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis. |
| title_full | Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis. |
| title_fullStr | Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis. |
| title_full_unstemmed | Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis. |
| title_short | Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis. |
| title_sort | efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis |
| url | http://europepmc.org/articles/PMC3440434?pdf=render |
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