Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliae
Summary Verticillium dahliae, a notorious phytopathogenic fungus, causes vascular wilt diseases in many plant species. The melanized microsclerotia enable V. dahliae to survive for years in soil and are crucial for its disease cycle. In a previous study, we characterized the secretory protein VdASP...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-07-01
|
| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14066 |
| _version_ | 1811226749581656064 |
|---|---|
| author | Cuimei Guo Xing Yang Hongli Shi Chi Chen Zhijuan Hu Xinyao Zheng Xingyong Yang Chengjian Xie |
| author_facet | Cuimei Guo Xing Yang Hongli Shi Chi Chen Zhijuan Hu Xinyao Zheng Xingyong Yang Chengjian Xie |
| author_sort | Cuimei Guo |
| collection | DOAJ |
| description | Summary Verticillium dahliae, a notorious phytopathogenic fungus, causes vascular wilt diseases in many plant species. The melanized microsclerotia enable V. dahliae to survive for years in soil and are crucial for its disease cycle. In a previous study, we characterized the secretory protein VdASP F2 from V. dahliae and found that VdASP F2 deletion significantly affected the formation of microsclerotia under adverse environmental conditions. In this study, we clarified that VdASP F2 is localized to the cell wall. However, the underlying mechanism of VdASP F2 in microsclerotial formation remains unclear. Transmembrane ion channel protein VdTRP was identified as a candidate protein that interacts with VdASP F2 using pull‐down assays followed by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis, and interaction of VdASP F2 and VdTRP was confirmed by bimolecular fluorescence complementary and coimmunoprecipitation assays. The deletion mutant was analysed to reveal that VdTRP is required for microsclerotial production, but it is not essential for stress resistance, carbon utilization and pathogenicity of V. dahliae. RNA‐seq revealed some differentially expressed genes related to melanin synthesis and microsclerotial formation were significantly downregulated in the VdTRP deletion mutants. Taken together, these results indicate that VdASP F2 regulates the formation of melanized microsclerotia by interacting with VdTRP. |
| first_indexed | 2024-04-12T09:30:35Z |
| format | Article |
| id | doaj.art-b72dbafbae2d4dc6a0481d26b088b5a5 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-12T09:30:35Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-b72dbafbae2d4dc6a0481d26b088b5a52022-12-22T03:38:22ZengWileyMicrobial Biotechnology1751-79152022-07-011572040205410.1111/1751-7915.14066Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliaeCuimei Guo0Xing Yang1Hongli Shi2Chi Chen3Zhijuan Hu4Xinyao Zheng5Xingyong Yang6Chengjian Xie7The Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations Chongqing Normal University Chongqing 401331 ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations Chongqing Normal University Chongqing 401331 ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations Chongqing Normal University Chongqing 401331 ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations Chongqing Normal University Chongqing 401331 ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations Chongqing Normal University Chongqing 401331 ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations Chongqing Normal University Chongqing 401331 ChinaCollege of Pharmacy Chengdu University Chengdu 610106 ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations Chongqing Normal University Chongqing 401331 ChinaSummary Verticillium dahliae, a notorious phytopathogenic fungus, causes vascular wilt diseases in many plant species. The melanized microsclerotia enable V. dahliae to survive for years in soil and are crucial for its disease cycle. In a previous study, we characterized the secretory protein VdASP F2 from V. dahliae and found that VdASP F2 deletion significantly affected the formation of microsclerotia under adverse environmental conditions. In this study, we clarified that VdASP F2 is localized to the cell wall. However, the underlying mechanism of VdASP F2 in microsclerotial formation remains unclear. Transmembrane ion channel protein VdTRP was identified as a candidate protein that interacts with VdASP F2 using pull‐down assays followed by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis, and interaction of VdASP F2 and VdTRP was confirmed by bimolecular fluorescence complementary and coimmunoprecipitation assays. The deletion mutant was analysed to reveal that VdTRP is required for microsclerotial production, but it is not essential for stress resistance, carbon utilization and pathogenicity of V. dahliae. RNA‐seq revealed some differentially expressed genes related to melanin synthesis and microsclerotial formation were significantly downregulated in the VdTRP deletion mutants. Taken together, these results indicate that VdASP F2 regulates the formation of melanized microsclerotia by interacting with VdTRP.https://doi.org/10.1111/1751-7915.14066 |
| spellingShingle | Cuimei Guo Xing Yang Hongli Shi Chi Chen Zhijuan Hu Xinyao Zheng Xingyong Yang Chengjian Xie Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliae Microbial Biotechnology |
| title | Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliae |
| title_full | Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliae |
| title_fullStr | Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliae |
| title_full_unstemmed | Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliae |
| title_short | Identification of VdASP F2‐interacting protein as a regulator of microsclerotial formation in Verticillium dahliae |
| title_sort | identification of vdasp f2 interacting protein as a regulator of microsclerotial formation in verticillium dahliae |
| url | https://doi.org/10.1111/1751-7915.14066 |
| work_keys_str_mv | AT cuimeiguo identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae AT xingyang identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae AT honglishi identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae AT chichen identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae AT zhijuanhu identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae AT xinyaozheng identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae AT xingyongyang identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae AT chengjianxie identificationofvdaspf2interactingproteinasaregulatorofmicrosclerotialformationinverticilliumdahliae |